Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye

Bilal Yakut; Ramazan Çetintaş; Tolga Gürkan; Betül Gürkan

doi:10.18016/ksutarimdoga.vi.1910433

TR EN

Diyarbakır İli Hevsel Bahçeleri Sebze Alanlarında Kök-ur Nematodlarının (Meloidogyne spp.) Tespiti ve Yayılımının Belirlenmesi

Öz

Hevsel Bahçeleri (Diyarbakır, Türkiye) sebze üretim alanlarında kök-ur nematodlarının varlığı ve yayılışı, 2019-2020 yıllarında yürütülen sürvey çalışmasıyla belirlenmiştir. Çalışmada 50 sebze üretim parseli incelenmiş, her parselden 5-10 bitkiye ait kök örneği alınmış ve toplam 455 bitki örneği değerlendirilmiştir. Urlu kökler kök-ur skalasına göre değerlendirilmiştir. Tür teşhisi, dişi bireylerden hazırlanan perineal pattern (anal kesit) preparatlarının ışık mikroskobu altında incelenmesi ve tanı anahtarlarıyla karşılaştırılması esas alınarak yapılmıştır. Morfolojik teşhisi desteklemek amacıyla esteraz izozim fenotiplemesi (EST-PAGE) uygulanmış ve bant paternleri referans fenotiplerle karşılaştırılmıştır. Her iki yöntem sonucunda kök-ur nematodlarının Meloidogyne javanica, M. incognita ve M. arenaria türlerine ait olduğu belirlenmiştir. EST-PAGE ile doğrulanan 22 popülasyon esas alındığında, türlerin yaygınlık oranları sırasıyla %63,6, %22,7 ve %13,6 olarak hesaplanmıştır. İncelenen 50 parselin 22’sinin (%44,0) kök-ur nematodları ile bulaşık olduğu saptanmıştır. Bulaşık parsellerden alınan 219 bitki örneğinin 203’ünde kök-ur nematodu tespit edilmiş ve bu parsellerde bitki örneği düzeyindeki bulaşıklık oranı %92,7 olarak hesaplanmıştır.

Anahtar Kelimeler

Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye

Öz

A survey conducted in vegetable production areas of the Hevsel Gardens (Diyarbakır, Türkiye) during 2019-2020 determined the occurrence and distribution of root-knot nematodes (Meloidogyne spp.). Fifty vegetable production plots were surveyed, 5-10 plants root samples were collected from each plot, and a total of 455 plant samples were evaluated. Galled roots were assessed using a root-galling scale. Species identification was based on the examination of perineal pattern preparations obtained from females under a light microscope and comparison with diagnostic keys. To support morphological identification, esterase isozyme phenotype (EST-PAGE) was performed, and banding patterns were compared with reference phenotypes. Both methods identified the detected root-knot nematodes as Meloidogyne javanica, M. incognita, and M. arenaria. Based on the 22 PAGE-confirmed populations, the prevalence rates of these species were 63.6%, 22.7%, and 13.6%, respectively. Of the 50 plots examined, 22 (44.0%) were infested with root-knot nematodes. Root-knot nematodes were detected in 203 of the 219 plant samples collected from the infested plots, representing a plant-sample infestation rate of 92.7% within infested plots.

Anahtar Kelimeler

Kaynakça

Abd-Elgawad, M. M. M. (2024). Upgrading strategies for managing nematode pests on profitable crops. Plants, 13(11), 1558. https://doi.org/10.3390/plants13111558.
Adam, M. A. M., Phillips, M. S., & Blok, V. C. (2007). Molecular diagnostic key for identification of single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathology, 56(1), 190-197. https://doi.org/10.1111/j.1365-3059.2006.01455.x
Aslan, A. & Elekçioğlu, İ. H. (2022). Biochemical and molecular identification of root-knot nematodes in greenhouse vegetable areas of Eastern Mediterranean Region (Turkey). Turkish Journal of Entomology, 46(1), 115-127. https://doi.org/10.16970/entoted.1055181
Aydınlı, G. & Mennan, S. (2016). Identification of root-knot nematodes (Meloidogyne spp.) from greenhouses in the Middle Black Sea Region of Turkey. Turkish Journal of Zoology, 40(5), 675-685. https://doi.org/10.3906/zoo-1508-19.
Bridge, J. & Page, S. L. J. (1980). Estimation of root-knot nematode infestation levels on roots using a rating chart. Tropical Pest Management, 26(3), 296–298. https://doi.org/10.1080/09670878009414416
Carneiro, R. M. D. G., Almeida, M. R. A. & Carneiro, R. G. (1996a). Enzyme phenotypes of Brazilian populations of Meloidogyne spp. Fundamental and Applied Nematology, 19(6), 555-560.
Carneiro, R. M. D. G., Carneiro, R. G., Abrantes, I. M. O., Santos, M. S. N. A. & Almeida, M. R. A. (1996b). Meloidogyne paranaensis n. sp. (Nemata: Meloidogynidae), a root-knot nematode parasitizing coffee in Brazil. Journal of Nematology, 28(2), 177-189.
Carneiro, R. M. D. G., Correa, V. R., Almeida, M. R. A., Gomes, A. C. M. M., Deimi, A. M., Castagnone-Sereno, P. & Karssen, G. (2014). Meloidogyne luci n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing different crops in Brazil, Chile and Iran. Nematology, 16, 289-301.

Cuvaca, J., Abrantes, I., Maleita, C. & Esteves, I. (2026). Occurrence and management of plant-parasitic nematodes in Mozambique: A review. Crops, 6(1), 6. https://doi.org/10.3390/crops6010006.
Eisenback, J. D., Hirschmann, H., Sasser, J. N. & Triantaphyllou, A. C. (1981). A guide to the four most common species of root-knot nematodes (Meloidogyne spp.) with a pictorial key. North Carolina State University, Raleigh, 48.
El Aimani, A., Houari, A., Laasli, S. E., Mentag, R., Iraqi, D., Diria, G., Khayi, S., Lahlali, R., Dababat, A. A. & Mokrini, F. (2022). Antagonistic potential of Moroccan entomopathogenic nematodes against root-knot nematodes, Meloidogyne javanica on tomato under greenhouse conditions. Scientific Reports, 12, 2915. https://doi.org/10.1038/s41598-022-07039-0.
Elling, A. A. (2013). Major emerging problems with minor Meloidogyne species. Phytopathology, 103(11), 1092-1102. https://doi.org/10.1094/PHYTO-01-13-0019-RVW.
Esbenshade, P. R. & Triantaphyllou, A. C. (1985). Use of enzyme phenotypes for identification of Meloidogyne species. Journal of Nematology, 17(1), 6-20.
FAO, 2025. FAOSTAT: Production: Crops and livestock products. Food and Agriculture Organization of the United Nations, Rome, Italy. Available at: https://www.fao.org/faostat/en/#data/QCL. Accessed 6 May 2026.
Fullana, A. M., Expósito, A., Escudero, N., Cunquero, M., Loza-Alvarez, P., Giné, A. & Sorribas, F. J. (2023). Crop rotation with Meloidogyne-resistant germplasm is useful to manage and revert the (a)virulent populations of Mi1.2 gene and reduce yield losses. Frontiers in Plant Science, 14, 1133095. https://doi.org/10.3389/fpls.2023.1133095
Gamalero, E. & Glick, B. R. (2020). The use of plant growth-promoting bacteria to prevent nematode damage to plants. Biology, 9(11), 381. https://doi.org/10.3390/biology9110381.
Gürkan, B., Çetintaş, R. & Gürkan, T. (2019). Gaziantep ve Osmaniye sebze alanlarında bulunan kök-ur nematodu türleri (Meloidogyne spp.)’nin teşhisi ile bazı nematod popülasyon ırklarının belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22(Ek Sayı 1), 113-124.
Hamad, H., Aydınlı, G. & Mennan, S. (2022). Distribution and prevalence of root-knot nematode species in greenhouse vegetables in northern Iraq. Turkish Journal of Entomology, 46(3), 359-369. https://doi.org/10.16970/entoted.1141029
Hartman, K. M. & Sasser, J. N. (1985). Identification of Meloidogyne species on the basis of differential host test and perineal-pattern morphology. In: K. R. Barker, C. C. Carter, & J. N. Sasser, (Eds.). An advanced treatise on Meloidogyne. Volume II: Methodology (pp. 69-77). North Carolina State University Graphics.
ICOMOS. (2015). Diyarbakır Fortress and Hevsel Gardens Cultural Landscape (Turkey) No. 1488. ICOMOS Advisory Body Evaluation. Retrieved May 8, 2026, from https://whc.unesco.org/document/152786.
İmren, M. & Elekçioğlu, İ. H. (2008). Diyarbakır ili buğday, sebze ve bağ alanlarında önemli bitki paraziti nematod türlerinin belirlenmesi. Çukurova Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 17(2), 116-121.
Janssen, T., Karssen, G., Verhaeven, M., Coyne, D. & Bert, W. (2016). Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype-based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports, 6, 22591. https://doi.org/10.1038/srep22591
Jepson, S. B. (1985). Meloidogyne chitwoodi. In: CIH descriptions of plant-parasitic nematodes (Set 8, No. 106). Commonwealth Agricultural Bureaux.
Kantor, M., Handoo, Z., Kantor, C. & Carta, L. (2022). Top ten most important U.S.-regulated and emerging plant-parasitic nematodes. Horticulturae, 8(3), 208. https://doi.org/10.3390/horticulturae8030208.
Karssen, G. (1996). Description of Meloidogyne fallax n. sp. (Nematoda: Heteroderidae), a root-knot nematode from The Netherlands. Fundamental and Applied Nematology, 19(6), 593-599.
Kaşkavalcı, G. & Öncüer, C. (1999). Investigations on distribution and economic importance of Meloidogyne goeldi, 1887 (Tylenchida: Meloidogynidae) species found in the major areas of hot climate vegetables in Aydın province. Turkish Journal of Entomology, 23(2), 149-160.
Mahmoud, A. M., El-Eslamboly, A. A., Adam, M., & Maraey, M. A. (2025). Biocontrol of Meloidogyne incognita and vegetative growth stimulation in tomato ‘Moneymaker’ plants by Egyptian soil bacteria. Egyptian Journal of Biological Pest Control, 35(1), 24. https://doi.org/10.1186/s41938-025-00860-5.
Meresa, B. K., Matthys, J. & Kyndt, T. (2024). Biochemical defense of plants against parasitic nematodes. Plants, 13(19), 2813. https://doi.org/10.3390/plants13192813
Mulk, M. M. (1976). Meloidogyne graminicola. In: CIH descriptions of plant-parasitic nematodes (Set 6, No. 87). Commonwealth Agricultural Bureaux.
Nagachandrabose, S., Shanthi, M. S. M., Shanmugam, S. P., Elaiyabharathi, T., Sharmila, R., Devrajan, K., Manickam, R. & Srinivasan, R. (2025). Integrating grafting and bio-inputs for sustainable management of root knot nematode, Meloidogyne incognita, in tomato cultivation. Frontiers in Plant Science, 16, 1623444. https://doi.org/10.3389/fpls.2025.1623444.
Orton Williams, K. J. (1972). Meloidogyne javanica. In: CIH descriptions of plant-parasitic nematodes (Set 1, No. 3). Commonwealth Agricultural Bureaux.
Orton Williams, K. J. (1973). Meloidogyne incognita. In: CIH descriptions of plant-parasitic nematodes (Set 2, No. 18). Commonwealth Agricultural Bureaux.
Orton Williams, K. J. (1974). Meloidogyne hapla. In: CIH descriptions of plant-parasitic nematodes (Set 3, No. 31). Commonwealth Agricultural Bureaux.
Orton Williams, K. J. (1975). Meloidogyne arenaria. In: CIH descriptions of plant-parasitic nematodes (Set 5, No. 62). Commonwealth Agricultural Bureaux.
Özarslandan, A. & Elekçioglu, I. H. (2010). Identification of the root-knot nematode species (Meloidogyne spp.) (Nemata: Meloidogynidae) collected from different parts of Turkey by moleculer and morphological methods. Turkish Journal of Entomology, 34(3), 323–335.
Page, S. L. J. (1985). Meloidogyne acronea. In: CIH descriptions of plant-parasitic nematodes (Set 8, No. 114). Commonwealth Agricultural Bureaux,
Phani, V., Khan, M. R. & Dutta, T. K. (2021). Plant-parasitic nematodes as a potential threat to protected agriculture: Current status and management options. Crop Protection, 144, 105573. https://doi.org/ 10.1016/j.cropro.2021.105573
Pires, D., Vicente, C. S. L., Menéndez, E., Faria, J. M. S., Rusinque, L., Camacho, M. J. & Inácio, M. L. (2022). The fight against plant-parasitic nematodes: Current status of bacterial and fungal biocontrol agents. Pathogens, 11(10), 1178. https://doi.org/10.3390/pathogens11101178.
Pulavarty, A., Egan, A., Karpinska, A., Horgan, K. & Kakouli-Duarte, T. (2021). Plant parasitic nematodes: A review on their behaviour, host interaction, management approaches and their occurrence in two sites in the republic of Ireland. Plants, 10(11), 2352. https://doi.org/10.3390/plants10112352
Rammah, A. & Hirschmann, H. (1988). Meloidogyne mayaguensis n. sp. (Meloidogynidae), a root-knot nematode from Puerto Rico. Journal of Nematology, 20, 58-69.
Resquín-Romero, G., Mattos, V. S., Monteiro, J. M. S., Lopez-Nicora, H. D., Amarilla, S. P., Chamorro-Diaz, S., Moral, J. & Carneiro, R. M. D. G. (2023). Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay. Agronomy, 13(3), 670. https://doi.org/10.3390/agronomy13030670.
Shahid, M. & Shafi, Z. (2025). Multi-omics and molecular engineering insights into Pseudomonas spp. mediated biocontrol of root-knot nematodes: An updated perspective. Physiological and Molecular Plant Pathology, 140, 102972. https://doi.org/10.1016/j.pmpp.2025.102972
Taylor, D. P. & Netscher, C. (1974). An improved technique for preparing perineal patterns of Meloidogyne spp. Nematologica, 20(2), 268-269.
UNESCO World Heritage Centre. (2015). Diyarbakır Fortress and Hevsel Gardens Cultural Landscape. Retrieved May 8, 2026, from https://whc.unesco.org/en/list/1488/.
Türkiye İstatistik Kurumu (TÜİK). (2024). Bitkisel üretim istatistikleri, 2024. TÜİK Veri Portalı.
Vasantha-Srinivasan, P., Park, K. B., Kim, K. Y., Jung, W. J. & Han, Y. S. (2025). The role of Bacillus species in the management of plant-parasitic nematodes. Frontiers in Microbiology, 15, 1510036. https://doi.org/10.3389/fmicb.2024.1510036.
Whitehead, A. G. (1968). Taxonomy of Meloidogyne (Nematodea: Heteroderidae) with descriptions of four new species. Transactions of the Zoological Society of London, 31, 263-401. https://doi.org/10.1111/j.1096-3642.1968.tb00368.x.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Nematoloji

Bölüm

Araştırma Makalesi

Yazarlar

Bilal Yakut
0000-0002-6414-1457
Türkiye

Ramazan Çetintaş
0000-0002-5738-6915
Türkiye

Tolga Gürkan ^*
0000-0003-0839-6559
Türkiye

Betül Gürkan
0000-0003-0195-4562
Türkiye

Erken Görünüm Tarihi

7 Kasım 2026

Yayımlanma Tarihi

-

Gönderilme Tarihi

16 Mart 2026

Kabul Tarihi

2 Haziran 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1910433

IZ

https://izlik.org/JA49RS43ZR

Kaynak Göster

RIS / Bibtex

APA

Yakut, B., Çetintaş, R., Gürkan, T., & Gürkan, B. (2026). Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1910433

AMA

1.Yakut B, Çetintaş R, Gürkan T, Gürkan B. Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1910433

Chicago

Yakut, Bilal, Ramazan Çetintaş, Tolga Gürkan, ve Betül Gürkan. 2026. “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1910433.

EndNote

Yakut B, Çetintaş R, Gürkan T, Gürkan B (07 Kasım 2026) Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]B. Yakut, R. Çetintaş, T. Gürkan, ve B. Gürkan, “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”, Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, Kas. 2026, doi: 10.18016/ksutarimdoga.vi.1910433.

ISNAD

Yakut, Bilal - Çetintaş, Ramazan - Gürkan, Tolga - Gürkan, Betül. “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (07 Kasım 2026). https://doi.org/10.18016/ksutarimdoga.vi.1910433.

JAMA

1.Yakut B, Çetintaş R, Gürkan T, Gürkan B. Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026. doi:10.18016/ksutarimdoga.vi.1910433.

MLA

Yakut, Bilal, vd. “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, Kasım 2026, doi:10.18016/ksutarimdoga.vi.1910433.

Vancouver

1.Bilal Yakut, Ramazan Çetintaş, Tolga Gürkan, Betül Gürkan. Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 07 Kasım 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1910433

Diyarbakır İli Hevsel Bahçeleri Sebze Alanlarında Kök-ur Nematodlarının (Meloidogyne spp.) Tespiti ve Yayılımının Belirlenmesi

Öz

Anahtar Kelimeler

Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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