Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.

Çiğdem Alev Özel; Siti Maesaroh

doi:10.18016/ksudobil.386167

TR EN

Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.

Abstract

Indigofera zollingeriana is an important protein rich forage and dye plant species that grows widely in Indonesia. It is difficult to propagate the plant through seeds due to high dormancy. Therefore, there is need to develop both in vitro and ex vitro propagation techniques of I. zollingeriana. This study aimed to develop a protocol for multiple shoot regeneration of the plant under in vitro conditions using different combinations of BAP+0.01 mg/L NAA. The results showed that both epigeal and cotyledon node explants have high potential to regenerate with maximum number of regenerated shoots noted on the epigeal and the cotyledon node explants using MS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0 mg/L BAP + 0.01 mg/L of NAA in the same order. The shoots regenerated on all combinations of BAP+NAA were rooted using 0.5 mg/L IBA that promoted rooting in effective manner. The rooted plants were easily acclimatized to external conditions in an environmental chamber. The results suggest that I. zollengriana plant species is no more difficult to regenerate under in vitro conditions. This research will aid in future studies related to breeding, propagation, biosynthesis and extraction of active compounds.

Keywords

auxin,cotyledon node,cytokinin,epigeal node,in vitro

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Çiğdem Alev Özel ^*

Siti Maesaroh

Publication Date

October 31, 2018

Submission Date

January 30, 2018

Acceptance Date

May 25, 2018

Published in Issue

Year 2018 Volume: 21 Number: 5

DOI

https://doi.org/10.18016/ksudobil.386167

IZ

https://izlik.org/JA97GL37PP

Cite

RIS / Bibtex

APA

Özel, Ç. A., & Maesaroh, S. (2018). Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(5), 672-677. https://doi.org/10.18016/ksudobil.386167

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Öz

Anahtar Kelimeler

Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

In vitro callus initiation from Indigofera (Indigofera zollingerianaMiq.)

Current status of the genetic and agronomic of industrial indigo Indigofera sp.