Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.

Çiğdem Alev Özel; Siti Maesaroh

doi:10.18016/ksudobil.386167

TR EN

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Öz

Indigofera zollingeriana, Endonezya'da yaygın olarak yetişen proteince zengin yem ve boya bitkisidir. Bitkiyi tohumdan çoğaltmak yüksek dormansi sebebiyle zor olmaktadır. Bu nedenle, I. zollingeriana'nın in vitro ve ex vitro koşulllarda çoğaltma tekniklerinin geliştirilmesi gerekmektedir. Bu çalışmada, farklı kombinasyonlarda BAP +0.01 mg/L NAA içeren MS ortamı kullanılarak in vitro koşullar altında bitkinin hızlı çoğaltım için bir protokol geliştirmesi amaçlanmıştır. En fazla sürgün rejenerasyonu epigeal ve kotiledon nodlarının sırasıyla 1.5 mg / L BAP + 0.01 mg / L NAA ve 1.0 mg/L BAP + 0.01 mg/L NAA içeren MS ortamında elde edilmiştir. BAP + NAA'nın tüm kombinasyonlarında rejenerasyonu yapılan sürgünler 0.5 mg/L IBA kullanılarak başarılı şekilde köklendirilmiştir. Köklü bitkiler, bir iklim dolabında dış koşullara alıştırılmıştır. Bu çalışma I. zollingeriana bitkisinin in vitro koşullar altında yetiştirilebileceğini göstermiş olması ve elde edilen sonuçların ilerideki çalışmalarda aktif bileşiklerin biyosentezi ve ekstraksiyonu ile bitki yetiştirilmesi ve çoğaltılmasına yardımcı olacağını düşünülmektedir.

Anahtar Kelimeler

oksin,kotiledon nod,sitokinin epigeal nod,in vitro

Kaynakça

Aasim M, Khawar KM, Ozcan S 2009. In vitro micro propagation from plumular apices of Turkish cowpea (Vigna unguiculata L.) cultivar Akkiz. Scientia Horticulturae, 122: 468-471.
Abdullah L 2010. Herbage production and quality of Indigofera treated by different concentration of foliar fertilizer. Media Peternakan, 33(3): 169-175.
Abdullah L, Apriastuti D, Apdini TAP 2012. Use of Indigofera zollingeriana as a forage protein source in dairy goat rations. The 1st Asia Dairy Goat Conference. 9-12 April 2012, Kuala Lumpur, Malaysia. Available at:www.fao.org/docrep/017/i2891e/i2891e02.pdf (Accesed 13 January 2017)
Abdullah L 2014. Prospektifagronomi dan ekofisiologi Indigofera zollingeriana sebagai tanaman penghasil hijauan pakan berkualitas tinggi. Pastura 3(2): 79-83.
Apdini TAP 2011. Utilization of Indigofera sp. Pellet for Etawah Crossbred and Saanen does in Bangun Karso farm. Bogor Agricultural University, Bachelor thesis, 45 p.
Baihaqi M, Widaningsih E, Fuah AM 2014. Influence of diets on milk production and compotition of etawah grade does reared in mined land reclamation. The 2nd Asian-Australasian Dairy Goat Conference. 25-27 April 2014, Bogor, Indonesia. Available at: http://repository.ipb.ac.id/ handle/ 123456789/76861
Bharal S, Rashid A 1978. Regeneration of plants from tissue culture of the legume Indigofera enneaphylla Linn. Zeitschrift für Pflanzenphysiologie, 92: 443-447.
Faradillah F, Mutia R, Abdullah L 2015. Subtitution of soybean meal with Indigofera zollingeriana top leaf meal on egg quality of Cortunix cortunix japonica. Media Peternakan 38(3): 192-197.

Flick CE, Evans DA, Sharp WR 1983. Organogenesis. (Handbook of plant cell culture, MacMillan Publish. Co., New York: Eds. Evans DA, Sharp WR, Ammirato PV, Yamada Y) 13-81.
Howell EC, Stewart CE, Evans PK 1986. Tissue culture and plant regeneration of Indigofera potaninii Craib. Journal of Plant Physiology, 128: 259-269.
Marina D 2012. Sperm quality of New Zealand white crossbred rabbit fed with complete ration containing Indigofera zollingeriana and Leucaena leucocephala leaves. http://repository.ipb.ac.id/ handle/123456789/57992. Pdf. (Date of access: 23.01.2017)..
Murashige T, Skoog F 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3): 473-497.
Nair DS, Reghunath BR 2009. Cryoconservation and regeneration of axillary shoot meristems of Indigofera tinctoria (L.) by encapsulation–dehydration technique. In vitro Cellular and Developmental Biology-Plant, 45: 565-573.
Nair DS, Reghunath BR, Soni KB, Alex S 2016. In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction. International Journal of Environment, Agriculture and Biotechnology, 1(4): 873-884.
Nofesa D 2012. Performance and viscera persentage of New Zealand white crossbred Rabbit Fed with complete feed containing Indigofera zollingeriana and Leucaena leucochepala leaves. http://repository.ipb.ac.id/handle/123456789/57837.pdf. (Date of access: 23.01.2017).
Ozel CA, Khawar KM, Karaman S, Ates MA, Arslan O 2008. Efficient in vitro multiplication in Ornithogalum ulophyllum Hand.-Mazz. from twin scale explants Scientia Horticulturae, 116(1): 109-112.
Ozel CA, Khawar KM, Unal F 2015. Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale. Saudi Journal of Biological Sciences, 22(2): 132-138.
Palupi R, Abdullah L, Astuti DA 2014. Potential and utilization of Indigofera sp. shoot leaf meal as soybean meal substitution in laying hen diets. Jurnal Ilmu Ternak dan Veteriner, 19 (3): 210-219.
Pardede RP 2012. Blood metabolic of UP3-Jonggol and Garut Rams fed ration containing Indigofera zollingeriana and waste bean sprouts. http://repository.ipb.ac.id/handle/123456789/60274. Pdf. (Date of access: 23.01.2017).
Phillips GC, Collins GB 1984. Red clover and other forage legumes. (Handbook of Plant Cell Culture, Vol 2. Macmillan, New York, NY, Eds. Sharp WR, Evans DA, Ammirato PV, Yamada Y) 169–210.
Rajabudeen E, Ganthi AS, Sivasubramanian S, Subramanian MPS 2014. In vitro regeneration of Indigofera viscosa Lam. Journal of Bio-Science, 22: 53-58.
Retnani Y, Permana IG, Komalasari NR, Roslina R, Ikhawanti A 2013. Biscuit bio-supplement for increasing milk production and quality in dairy goat farm. Asian Journal of Animal Sciences, 8(1): 15-23.
Retnani Y, Permana IG, Purba LC 2014. Physical characteristic and palatability of biscuit bio-supplement for dairy goat. Pakistan Journal of Biological Sciences, 17(5): 725-729.
Sambas ID 2012. The protein utilization efficiency of complete feed with Indigofera zollingeriana and sprout bean waste in fattening of local ram. http://repository.ipb.ac.id/handle/123456789/57991. (Date of access: 23.01.2017).
Santi MA, Sumiati Abdullah L. 2015. Cholesterol and malondialdehyde contents of broiler-chicken meat supplemented with Indigofera zolingeriana top leaf meal. Media Peternakan, 38(3): 163-168.
Senthilkumar M, Vinothkumar D, Anupama M, Anandakumar D 2015. In vitro propagation of Indigofera trita L. F-Highly reputed medicinal plants. International Journal of Pure and Applied Bioscience, 3(4): 276-279.
Snedecor GW, Cochran WG 1976. ‘Statistical methods 6th edition’. Oxford and IBH Publishing: New Delhi, 503 p. Tejavathi DH, Devaraj VR, Murthy SM, Anitha P, Nijagunaiah R 2010. Regeneration of multiple shoots from the callus cultures of Macrotyloma uniflorum (Lam) Verdc. Indian Journal of Biotechnology, 9: 101-10.
Varisai Mohamed S, Jawahar M, Jayabalan N 1998. Effect of AdS. BAP and IBA on plant regeneration from Macrotyloma uniflorum (Lam.) Verdc. Phytomorphology, 48: 61–65.
Varisai Mohamed S, Jawahar M, Thiruvengadam M, Jeyakumar M, Jayabalan N 1999. Effect of cytokinins on proliferation of multiple shoots in horsegram [Macrotyloma uniflorum (Lam.) Verdc.]. Journal Plant Biotechnology, 1 (2): 79–83.
Wiriadinata H 2012. Indigofera L. (Papilionaceae) di Indonesia. (Indigofera sebagai pakan ternak, IAARD Press, Jakarta Indonesia: Eds. SP Ginting, BR Prawiradipura, ND Purwantari) 9-24

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Çiğdem Alev Özel ^*

Siti Maesaroh

Yayımlanma Tarihi

31 Ekim 2018

Gönderilme Tarihi

30 Ocak 2018

Kabul Tarihi

25 Mayıs 2018

Yayımlandığı Sayı

Yıl 1970 Cilt: 21 Sayı: 5

DOI

https://doi.org/10.18016/ksudobil.386167

IZ

https://izlik.org/JA97GL37PP

Kaynak Göster

RIS / Bibtex

APA

Özel, Ç. A., & Maesaroh, S. (2018). Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(5), 672-677. https://doi.org/10.18016/ksudobil.386167