Patlıcan Tohumlarında Bitki Büyüme Düzenleyici Rizobakteri Uygulamalarının Kurşuni Küf (Botrytis cinerea Pers.: Fr.) Hastalığına Etkileri

Hacer Handan Altınok; Gülperi Çifçi

doi:10.18016/ksutarimdoga.vi.492824

EN TR

Effects of Plant Growth Promoting Rhizobacteria Treatments of Eggplant Seeds Against Grey Mold (Botrytis cinerea Pers.: Fr.) Disease

Abstract

Grey mold disease of eggplant (Botrytis cinerea Pers.) causes serious yield losses on eggplant (Solanum melongena L.) production in greenhouses in Turkey. In this study, effects of some plant growth regulating rhizobacteria against grey mold disease were investigated.Bacillus spp. (B379c, B10a and 76A-1) and Pseudomonas spp. (P07-1, P07-4 and 85A-2) isolates inhibited mycelial development of B. cinerea (Bc-TR07) under in vitro conditions at varying rates (21-33%). Among the PGPR isolates, P. aeruginosa (P07-1) was found as the most successful isolate in pot experiments with an efficiency rate of 58.1%, compared to positive control containing only pathogen inoculum. Similarly, this isolate significantly increased some plant growth parameters. The P07-1 isolate resulted a 27.0% increase in total proline content of eggplants 72 h after pathogen inoculation, and in the same period, defense enzymes catalase (CAT) and peroxidase (POX) were also increased 22.8% and 27.7% respectively, compared to positive control. This isolate was followed by P. putida (P11-4) and then B. amyloliquefaciens (76A-1) by means of CAT and POX enzymes activity. The major disease suppression mechanisms of the tested PGPR isolates were identified as their plant growth regulation and resistance induction abilities.

Keywords

Grey mold,PGPR,Induced resistance,Eggplant

Patlıcan Tohumlarında Bitki Büyüme Düzenleyici Rizobakteri Uygulamalarının Kurşuni Küf (Botrytis cinerea Pers.: Fr.) Hastalığına Etkileri

Öz

Ülkemizde örtü altı patlıcan (Solanum melongena L.) üretiminde kurşuni küf hastalığı (Botrytis cinerea Pers.) önemli verim kayıplarına neden olmaktadır. Bu çalışmada, kurşuni küf hastalığına karşı bazı bitki gelişme düzenleyici rhizobakterilerin etkinlikleri araştırılmıştır. Bacillus spp. (B379c, B10a ve 76A-1) ve Pseudomonas spp. (P07-1, P07-4 ve 85A-2) izolatları in vitro koşullarda B. cinerea’nın (Bc-TR07) miseliyal gelişimini %21-33 oranında engellemiştir. PGPR izolatları arasında P. aeruginosa (P07-1), saksı denemelerinde sadece patojen inokulumu içeren pozitif kontrole göre %58.1 etki oranı ile en başarılı izolat olarak belirlenmiştir. Bu izolat bazı bitki gelişim parametrelerini de benzer şekilde önemli oranda arttırmıştır. P07-1 izolatı, patojen inokulasyonundan 72 saat sonra patlıcan bitkilerinde toplam prolin içeriğini pozitif kontrole göre %27.0 oranında artırırken, aynı sürede savunma enzimlerinden katalaz (CAT) ve peroksidazı (POX) sırasıyla %22.8 ve %27.7 oranında artırmıştır. Bu izolatı CAT ve POX enzim aktiviteleri açısından sırasıyla P. putida (P11-4) ve B. amyloliquefaciens (76A-1) takip etmiştir. Test edilen PGPR izolatlarının başlıca hastalık baskılama mekanizmalarının, bitki büyüme düzenleyici ve dayanıklılığı uyarıcı özellikleri olduğu belirlenmiştir

Anahtar Kelimeler

Kurşuni küf,PGPR,Uyarılmış dayanıklılık,Patlıcan

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Hacer Handan Altınok ^*
0000-0002-4267-1107
Türkiye

Gülperi Çifçi
0000-0002-2568-9647
Türkiye

Publication Date

June 30, 2019

Submission Date

December 6, 2018

Acceptance Date

March 4, 2019

Published in Issue

Year 2019 Volume: 22 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.492824

IZ

https://izlik.org/JA83YF44YF

Cite

RIS / Bibtex

APA

Altınok, H. H., & Çifçi, G. (2019). Patlıcan Tohumlarında Bitki Büyüme Düzenleyici Rizobakteri Uygulamalarının Kurşuni Küf (Botrytis cinerea Pers.: Fr.) Hastalığına Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22(3), 421-429. https://doi.org/10.18016/ksutarimdoga.vi.492824

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