Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi

Sabahattin Cömertpay; Gizem Ceylan

doi:10.18016/ksutarimdoga.vi.496108

TR EN

Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi

Öz

Malignant mezoteliyoma akciğer, kalp ve karın zarında gelişen bir kanser türüdür. Kapsaisin, Capsicum spp.cinsi bitkilerde üretilen ikincil bir metabolittir ve bazı kanser hücreleri üzerinde sitotoksik etkisi olduğu gösterilmiştir. Afidokolin ve nokadozol, hücreleri sırasıyla G1/S ve G2/M fazında tutar. Bu çalışmada, kanserli (H2452) ve ölümsüzleştirilmiş (AG07086) mezotelyum hücrelerinin kapsaisin sitotoksisitesi, afidokolin ve nokodazol ön muamelesi ile, ya da bu muameleler olmaksızın, MTS tekniğiyle belirlenmiş ve kaspaz-3 mRNA seviyesindeki değişiklikler qRT-PCR metoduyla incelenmiştir. Elde edilen bulgulara göre; i) normal koşullarda, kanserli ve ölümsüzleştirilmiş mezotelyum hücrelerinde kapsaisin sitotokisitesi benzerdir (p>0.05), ii) afidikolin muamelesiyle kapsaisinin etkenliği azalmış (p<0.05) ancak kanser hücrelerinin kapsaisin duyarlılığı ölümsüz hücrelere kıyasla yükselmiştir (p<0.05), iii) nokodazol ön muamelesi ile, kanser hücrelerinde kapsaisinin sitotoksik etkisi düşüş göstermiş (p<0.05), ancak ölümsüzleştirilmiş hücreler için bu değişim istatiksel olarak anlamsız bulunmuştur (p>0.05), iv) yapılan tüm uygulamalarda kaspaz-3 mRNA miktarı kontrol grubuna denk çıkmıştır (p>0.05).

Anahtar Kelimeler

Mezotelyoma,Kapsaisin,Afidikolin,Nokodazol,Sitotoksisite

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Sabahattin Cömertpay ^*
0000-0003-4850-6927
Türkiye

Gizem Ceylan
0000-0001-6281-4665
Türkiye

Publication Date

June 30, 2019

Submission Date

December 12, 2018

Acceptance Date

February 15, 2019

Published in Issue

Year 2019 Volume: 22 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.496108

IZ

https://izlik.org/JA92BN49DM

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RIS / Bibtex

APA

Cömertpay, S., & Ceylan, G. (2019). Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22(3), 456-465. https://doi.org/10.18016/ksutarimdoga.vi.496108

Cited By

Kapsaisinin H2452 Mezotelyoma Hücre Hattından Seçilen Klonlarda Sitotoksisitesi ve Kaspaz-3 Üzerine Etkilerinin Belirlenmesi

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.673591

Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi

Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi

Öz

Anahtar Kelimeler

Effect of Nocodazole and Aphidicolin Pre-treatment on Capsaicin Cytotoxicity in Malignant and Immortalized Cells of Mesothelium

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Kapsaisinin H2452 Mezotelyoma Hücre Hattından Seçilen Klonlarda Sitotoksisitesi ve Kaspaz-3 Üzerine Etkilerinin Belirlenmesi