Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Yüksel Aliyazıcıoğlu; Selim Demir; Serap Özer Yaman; Sıla Özlem Şener; Elif Ayazoğlu Demir; Rezzan Aliyazıcıoğlu; İbrahim Turan

doi:10.18016/ksutarimdoga.vi.579938

EN TR

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Abstract

The purpose of this study was to investigate the phytochemical analysis of D. pentaphyllum and its cytotoxic activity on human cervical cancer (HeLa) cells with possible mechanisms. Total phenolic contents (TPC) and phytochemical analysis of the extract were evaluated using spectrophotometric method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism of the cytotoxic effect of the extract was also evaluated in terms of apoptosis, cell cycle, and mitochondrial membrane potential (MMP) using the fluorometric methods. The TPC of the extract was calculated as 68.9±2.3 mg gallic acid equivalent per g sample and quercetin was determined as the major phenolic in the extract. The extract showed a selective cytotoxic effect (6.5-fold) on HeLa cells compared to normal fibroblast cells. The cytotoxic effect of the extract was found to be due to the arresting of the cell cycle in the S phase and increasing apoptosis through decreased MMP. Further studies are needed for the comprehensive effects of the extract on cell signaling.

Keywords

Apoptosis,Cell cycle,Cervix cancer,Cytotoxicity,Dorycnium pentaphyllum

References

Aliyazicioglu Y, Demir S, Turan I, Cakiroglu TN, Akalin I, Deger O, Bedir A, 2011. Preventive and protective effects of Turkish propolis on H2O2-induced DNA damage in foreskin fibroblast cell lines. Acta Biol Hung, 62(4): 388-396.
Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N, Demir EA, Ozgen U, Karaoglu SA, Aliyazicioglu Y, 2017. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod, 11(3): 270-284.
Bencherchar I, Demirtas I, Altun M, Gul F, Sarri D, Benayache F, Benayache S, Mekkiou R, 2017. HPLC analysis, anti-oxidant activity of Genista ferox and its antiproliferative effect in HeLa cell line. Bangladesh J Pharmacol, 12: 260-267.
Bremner P, Rivera D, Calzado MA, Obón C, Inocencio C, Beckwith C, Fiebich BL, Muñoz E, Heinrich M, 2009. Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators. J Ethnopharmacol, 124(2): 295-305.
Chaddha V, Jain PK, Dixit ND, Rathor H, Zama R, 2018. An importance of herbal medicines in treatment of cancer disease. Int J Pharm Sci Rev Res, 51(2): 80-82.
Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O, 2016a. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer, 68(1): 165-172.
Demir S, Turan I, Aliyazicioglu Y, 2016b. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells. J Buon, 21(4): 883-888.
Demir S, Turan I, Aliyazicioglu Y, Kilinc K, Yaman SO, Demir EA, Arslan A, Mentese A, Deger O, 2017a. Morus rubra extract induces cell cycle arrest and apoptosis in human colon cancer cells through endoplasmic reticulum stress and telomerase. Nutr Cancer, 69(1): 74-83.

Demir S, Turan I, Demir F, Demir EA, Aliyazicioglu Y, 2017b. Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharm J, 21(1): 121-126.
Demir S, Turan I, Aliyazicioglu R, Yaman SO, Aliyazicioglu Y, 2018a. Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells. J Pharm Anal, 8(5): 307-311.
Demir S, Turan I, Aliyazicioglu Y, 2018b. Cytotoxic effect of Rhododendron luteum leaf extract on human cancer cell lines. KSU J Agric Nat, 21(6): 950-956.
Demir S, Turan I, Misir S, Aliyazicioglu Y, 2019a. Selective cytotoxic effect of Dorycnium pentaphyllum extract on human breast, liver, and lung cancer cells. KSU J Agric Nat, 22(3): 473-479.
Demir S, Turan I, Aliyazicioglu Y, 2019b. Antioxidant properties of Primula vulgaris flower extract and its cytotoxic effect on human cancer cell lines. KSU J Agric Nat, 22(1): 78-84.
Di Domenico F, Foppoli C, Coccia R, Perluigi M, 2012. Antioxidants in cervical cancer: chemopreventive and chemotherapeutic effects of polyphenols. Biochim Biophys Acta, 1822(5): 737-747.
Hussein SAM, El-Mesallamy AMD, Souleman AMA, Mousa MA, 2016. Cytotoxic activity of bioactive compound from Caesalpinia ferrea Martius, Fabaceae. IJPPR, 8(12): 2080-2084.
Jantova S, Nagy M, Ruzeková L, Grancai D, 2001. Cytotoxic effects of plant extracts from the families Fabaceae, Oleaceae, Philadelphaceae, Rosaceae and Staphyleaceae. Phytother Res, 15(1): 22-25.
Kim C, Kim B, 2018. Anti-cancer natural products and their bioactive compounds inducing ER stress-mediated apoptosis: A review. Nutrients, 10(8): 1021.
Kumar RS, Murugananthan G, Rajkapoor B, 2017. Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth. (Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats. Braz J Biol Sci, 4(7): 53-66.
Lacerda LP, Malaquias G, Peron AP, 2014. Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L. An Acad Brasil Cienc, 86(3): 1147-1150.
Mentese U, Dogan OV, Turan I, Usta S, Dogan E, Mentese SO, Demir S, Ozer T, Aykan AC, Alver A, 2014. Oxidant-antioxidant balance during on-pump coronary artery bypass grafting. Scientific World Journal, Article ID: 263058, doi: 10.1155/2014/263058.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA, 2000. Comparison of the free radical- scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71(1-2): 109-114.
Mosmann T, 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55-63.
Naghibi F, Irani M, Hassanpour A, Pirani A, Hamzeloo-Moghadam M, 2014. Cytotoxic effects of selective species of Caryophyllaceae in Iran. RJP, 1(2): 29-32.
Noufou O, Sawadogo WR, Tibiri A, Lompo M, Dijoux MG, Diederich M, Guissou IP, 2016. Phenolic contents and in vitro pharmacological activities of methanolic extract of Pterocarpus erinaceus Poir. stem bark (Fabaceae). BJPR, 10(2): 1-7.
Oyenihi AB, Smith C, 2019. Are polyphenol antioxidants at the root of medicinal plant anti-cancer success? J Ethnopharmacol, 229: 54-72.
Ozkan MT, Aliyazicioglu R, Demir S, Misir S, Turan I, Yildirmis S, Aliyazicioglu Y, 2017. Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells. Jundishapur J Nat Pharm Prod, 12(1): e40073.
Rathi SG, Suthar M, Patel P, Bhaskar VH, Rajgor NB, 2009. In-vitro cytotoxic screening of Glycyrrhiza glabra L. (Fabaceae): a natural anticancer drug. J Young Pharm, 1(3): 239-243.
Saha S, Shilpi JA, Mondal H, Hossain F, Anisuzzman M, Hasan MM, Cordell GA, 2013. Ethnomedicinal, phytochemical, and pharmacological profile of the genus Dalbergia L. (Fabaceae). Phytopharmacology, 4(2): 291-346.
Slinkard K, Singleton VL, 1977. Total phenol analyses: automation and comparison with manual methods. Am J Enol Viticult, 28: 49-55.
Sobeh M, El-Hawary E, Peixoto H, Labib RM, Handoussa H, Swilam N, El-Khatib AH, Sharapov F, Mohamed T, Krstin S, Linscheid MW, Singab AN, Wink M, Ayoub N, 2016. Identification of phenolic secondary metabolites from Schotia brachypetala Sond. (Fabaceae) and demonstration of their antioxidant activities in Caenorhabditis elegans. Peer J, 4: e2404.
Soni S, Vaidya S, Jain AK, Sharma A, 2013. A comparative study on anticancer potential of different extracts of Mucuna pruriens Linn. seeds against sertoli (GC) and ZR-75 cell lines. IJPCBS, 3(2): 305-314.
Stefanović OD, Tešić JD, Čomić LR, 2015. Melilotus albus and Dorycnium herbaceum extracts as source of phenolic compounds and their antimicrobial, antibiofilm, and antioxidant potentials. J Food Drug Anal, 23(3): 417-424.
Turan I, Deger O, Aliyazicioglu Y, Demir S, Kilinc K, Sumer A, 2015. Effects of Turkish propolis on expression of hOGG-1 and NEIL-1. Turk J Med Sci, 45(4): 804-811.
Turan I, Demir S, Kilinc K, Burnaz NA, Yaman SO, Akbulut K, Mentese A, Aliyazicioglu Y, Deger O, 2017a. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells. Saudi Pharm J, 25(2): 241-248.
Turan I, Demir S, Kilinc K, Aliyazicioglu Y, Alver A, Misir S, Yaman SO, Akbulut K, Mentese A, Deger O, 2017b. Morus rubra extract induces G1 cell cycle arrest and apoptosis in human lung and prostate cancer cells. IJPER, 51(1): 51-58.
Turan I, Demir S, Kilinc K, Yaman SO, Misir S, Kara H, Genc B, Mentese A, Aliyazicioglu Y, Deger O, 2018. Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression. J Pharm Anal, 8(6): 394-399.
Yalcin CO, Aliyazicioglu Y, Demir S, Turan I, Bahat Z, Misir S, Deger O, 2016. Evaluation of radioprotective effect of Turkish propolis on foreskin fibroblast cells. J Cancer Res Ther, 12(2): 990-994.
Zhang J, Nishimoto Y, Tokuda H, Suzuki N, Yasukawa K, Kitdamrongtham W, Akazawa H, Manosroi A, Manosroi J, Akihisa T, 2013. Cancer chemopreventive effect of bergenin from Peltophorum pterocarpum wood. Chem Biodivers, 10(10): 1866-1875.
Zingue S, Tchoumtchoua J, Ntsa DM, Sandjo LP, Cisilotto J, Nde CBM, Winter E, Awounfack CF, Ndinteh DT, Clyne C, Njamen D, Halabalaki M, Creczynski-Pasa TB, 2016. Estrogenic and cytotoxic potentials of compounds isolated from Millettia macrophylla Benth (Fabaceae): towards a better understanding of its underlying mechanisms. BMC Complement Altern Med, 16(1): 421

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Serap Özer Yaman
0000-0002-5089-0836
Türkiye

Sıla Özlem Şener
0000-0003-2811-4707
Türkiye

Elif Ayazoğlu Demir
0000-0001-9027-7633
Türkiye

Rezzan Aliyazıcıoğlu
0000-0003-2913-2528
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Publication Date

December 31, 2019

Submission Date

June 19, 2019

Acceptance Date

August 1, 2019

Published in Issue

Year 2019 Volume: 22

DOI

https://doi.org/10.18016/ksutarimdoga.vi.579938

IZ

https://izlik.org/JA88ZC24NX

Cite

RIS / Bibtex

APA

Aliyazıcıoğlu, Y., Demir, S., Özer Yaman, S., Şener, S. Ö., Ayazoğlu Demir, E., Aliyazıcıoğlu, R., & Turan, İ. (2019). Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22, 365-373. https://doi.org/10.18016/ksutarimdoga.vi.579938

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Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Abstract

Keywords

Dorycnium pentaphyllum'un Fitokimyasal Analizi ve Serviks Kanseri Hücreleri Üzerindeki Antiproliferatif Etkisi

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

An Investigation of the Antiproliferative Effect of Rhododendron luteum Extract on Cervical Cancer (HeLa) Cells via Nrf2 Signaling Pathway

The therapeutic effect of silibinin against 5‐fluorouracil‐induced ovarian toxicity in rats

Therapeutic Effect of Sinapic Acid against 5-Fluorouracil-Induced Oxidative Stress and Inflammation in Rat Ovarium: An Experimental Approach

N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats

Suppression of cisplatin-induced ovarian injury in rats by chrysin: an experimental study

Therapeutic Effect of Astaxanthin on 5-Fluorouracil-Induced Ovarian Damage in Rats

Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması