Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Yüksel Aliyazıcıoğlu; Selim Demir; Serap Özer Yaman; Sıla Özlem Şener; Elif Ayazoğlu Demir; Rezzan Aliyazıcıoğlu; İbrahim Turan

doi:10.18016/ksutarimdoga.vi.579938

EN TR

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Abstract

The purpose of this study was to investigate the phytochemical analysis of D. pentaphyllum and its cytotoxic activity on human cervical cancer (HeLa) cells with possible mechanisms. Total phenolic contents (TPC) and phytochemical analysis of the extract were evaluated using spectrophotometric method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism of the cytotoxic effect of the extract was also evaluated in terms of apoptosis, cell cycle, and mitochondrial membrane potential (MMP) using the fluorometric methods. The TPC of the extract was calculated as 68.9±2.3 mg gallic acid equivalent per g sample and quercetin was determined as the major phenolic in the extract. The extract showed a selective cytotoxic effect (6.5-fold) on HeLa cells compared to normal fibroblast cells. The cytotoxic effect of the extract was found to be due to the arresting of the cell cycle in the S phase and increasing apoptosis through decreased MMP. Further studies are needed for the comprehensive effects of the extract on cell signaling.

Keywords

Apoptosis,Cell cycle,Cervix cancer,Cytotoxicity,Dorycnium pentaphyllum

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Serap Özer Yaman
0000-0002-5089-0836
Türkiye

Sıla Özlem Şener
0000-0003-2811-4707
Türkiye

Elif Ayazoğlu Demir
0000-0001-9027-7633
Türkiye

Rezzan Aliyazıcıoğlu
0000-0003-2913-2528
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Yayımlanma Tarihi

31 Aralık 2019

Gönderilme Tarihi

19 Haziran 2019

Kabul Tarihi

1 Ağustos 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 22

DOI

https://doi.org/10.18016/ksutarimdoga.vi.579938

IZ

https://izlik.org/JA88ZC24NX

Kaynak Göster

RIS / Bibtex

APA

Aliyazıcıoğlu, Y., Demir, S., Özer Yaman, S., Şener, S. Ö., Ayazoğlu Demir, E., Aliyazıcıoğlu, R., & Turan, İ. (2019). Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22, 365-373. https://doi.org/10.18016/ksutarimdoga.vi.579938

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Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Abstract

Keywords

Dorycnium pentaphyllum'un Fitokimyasal Analizi ve Serviks Kanseri Hücreleri Üzerindeki Antiproliferatif Etkisi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

An Investigation of the Antiproliferative Effect of Rhododendron luteum Extract on Cervical Cancer (HeLa) Cells via Nrf2 Signaling Pathway

The therapeutic effect of silibinin against 5‐fluorouracil‐induced ovarian toxicity in rats

Therapeutic Effect of Sinapic Acid against 5-Fluorouracil-Induced Oxidative Stress and Inflammation in Rat Ovarium: An Experimental Approach

N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats

Suppression of cisplatin-induced ovarian injury in rats by chrysin: an experimental study

Therapeutic Effect of Astaxanthin on 5-Fluorouracil-Induced Ovarian Damage in Rats

Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması

e-ISSN: 2619-9149