Assessment of Genetic Diversity in Cucumber (Cucumis sativus L.) Genotypes Using Morphological Characters and AFLP Analysis

Yaşar Karakurt; Damla Güvercin; Sercan Önder; Özgür İşler

doi:10.18016/ksutarimdoga.vi.583844

TR EN

Hıyar (Cucumis sativus L.) Genotiplerinin Morfolojik Karakterler ve AFLP Analizi ile Genetik Çeşitliliğinin Değerlendirilmesi

Öz

Bu çalışmanın amacı hıyar genotiplerinin morfolojik ve moleküler çeşitliliğini değerlendirmektir. Bu amaçla, altı adet morfolojik özellik (bitki morfolojisi, bitki boyu, yaprak ayası büyüklüğü, meyve uzunluğu, meyve çapı ve meyve sapı uzunluğu) ve sekiz AFLP markeri kullanılarak 18 hıyar hattı genetik çeşitlilik açısından değerlendirilmiştir. Kullanılan AFLP primer kombinasyonları polimorfizm göstermiştir. Çalışma sonucunda 1975 AFLP fragmanı elde edilmiş ve 1468 fragmanın polimorfik olduğu görülmüştür (%75.34). Dendrogramlar, aritmetik ortalamalar kullanılarak UPGMA (Unweighted Pair Group Method) yöntemiyle çizilmiş ve UPGMA dendrogramına göre hıyar genotipleri iki ana gruba ayrılmıştır. Dendrogramın genetik mesafeleri 0.92 ile 0.96 arasında değişmiştir. Morfolojik verilere dayanan küme analizinde ise UPGMA dendrogramı tüm hatları üç ana kümeye ayırmıştır. Çalışmada kullanılan hıyar hatlarının benzerlik katsayısının 0.888 ile 0.982 arasında değişen düşük bir genetik varyasyon seviyesine sahip olduğu belirlenmiştir. 8 çift AFLP primeri ile oluşturulan filogenetik dendogramlarla, morfolojik taksonomi ile yapılan UPGMA kümeleme analizleriyle oluşturulan dendogramlar örtüşmektedir.

Anahtar Kelimeler

AFLP,Hıyar,Genetik uzaklık,Akrabalık

Assessment of Genetic Diversity in Cucumber (Cucumis sativus L.) Genotypes Using Morphological Characters and AFLP Analysis

Abstract

For this purpose, 18 cucumber lines were evaluated for their genetic diversity using six morphological characterizations (plant morphology, plant length, length of leaf blade, fruit length, fruit diameter and fruit stem length) and eight AFLP markers. These AFLP primer combinations amplified well and also showed polymorphism. Thus, 1975 AFLP fragments were obtained and 1468 fragments were polymorphic (75.34%). Dendrograms were drawn using UPGMA (Unweighted Pair Group Method) arithmetical averages and according to the UPGMA dendrogram, the cucumber accessions clustered into two main groups. The genetic distances of the dendrogram varied between 0.92 and 0.96. Cluster analysis based on morphological data discriminated all lines into three major clusters in UPGMA dendrogram. The similarity coefficient ranged between 0.888 and 0.982 indicating that the cucumber lines used in the study have a low level of genetic variation. Results obtained from the phylogenetic dendrogram by 8 pairs of AFLP primers were consistent with those from the UPGMA clustering analysis, which were in according with the morphological taxonomy on cucumber.

Keywords

AFLP,Cucumis sativus,Genetic diversity,Relationships

Supporting Institution

Suleyman Demirel University.

Thanks

Funding for this work was provided by a grant from Suleyman Demirel University.

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Yaşar Karakurt
0000-0003-3914-0652
Türkiye

Damla Güvercin ^*
0000-0002-6639-3818

Sercan Önder
0000-0002-8065-288X

Özgür İşler
0000-0001-8835-2526

Publication Date

June 30, 2020

Submission Date

June 28, 2019

Acceptance Date

January 9, 2020

Published in Issue

Year 2020 Volume: 23 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.583844

IZ

https://izlik.org/JA46ML68PN

Cite

RIS / Bibtex

APA

Karakurt, Y., Güvercin, D., Önder, S., & İşler, Ö. (2020). Assessment of Genetic Diversity in Cucumber (Cucumis sativus L.) Genotypes Using Morphological Characters and AFLP Analysis. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(3), 577-585. https://doi.org/10.18016/ksutarimdoga.vi.583844

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