Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis
Abstract
Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production
quite challenging. In this study, effects of three concentrations of
6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different
amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F.
imperialis plantlets in vitro were determined. The auxins were at
similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all
treatments. It has been found that the size and number of bulblets, as well as
root and shoot numbers in regenerated plantlets were increased significantly
(p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10
mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots
in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L)
decreased the bulblet number in all treatments, however, when supplemented with
3.5 g/L of AC, it was found to raise all the values measured (p<0.05).
Overall, the best growth of the plant parts from callus were achieved with the
highest concentration of AC and 1.5 mg/L of BAP.
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References
- Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endongered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity Conservation 14(5):1059-1069.
- Anagnostakis S 1974. Haploid plants from anthers of tobacco Enhancement with charcoal. Planta 155(3):281-283.
- Arditti J, Krikorian AD 1996. Orchid micropropagation: the path from laboratory to commercialization and an account of several unprecedented investigators. Botanical Journal of the Linnean Society 122(3):183-241.
- Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousiabtryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland Australia. Biodiversity Conservation 11(8):1469-1477.
- Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue Organ Culture 40(3):231-235.
- Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular & Developmental Biology-Plant 42(5):455-460.
- Fridborg G, Eriksson T 1975. Effects of Activated Charcoal on Growth and Morphologenesis in Cell Cultures. Physiologia Plantarum. 34(4):306-308.
- Fridborg G, Pedersen M, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum 43(2):104-106.
Details
Primary Language
English
Subjects
Agricultural, Veterinary and Food Sciences
Journal Section
Research Article
Publication Date
April 30, 2020
Submission Date
September 30, 2019
Acceptance Date
November 8, 2019
Published in Issue
Year 2020 Volume: 23 Number: 2
