Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis
Öz
Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production
quite challenging. In this study, effects of three concentrations of
6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different
amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F.
imperialis plantlets in vitro were determined. The auxins were at
similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all
treatments. It has been found that the size and number of bulblets, as well as
root and shoot numbers in regenerated plantlets were increased significantly
(p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10
mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots
in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L)
decreased the bulblet number in all treatments, however, when supplemented with
3.5 g/L of AC, it was found to raise all the values measured (p<0.05).
Overall, the best growth of the plant parts from callus were achieved with the
highest concentration of AC and 1.5 mg/L of BAP.
Anahtar Kelimeler
Destekleyen Kurum
Proje Numarası
Teşekkür
Kaynakça
- Almeida R, Goncalves S, Romano A 2005. In vitro micropropagation of endongered Rhododendron Ponticum L. subsp. Baeticum (Boissier and Reuter) Handel-Mazzetti. Biodiversity Conservation 14(5):1059-1069.
- Anagnostakis S 1974. Haploid plants from anthers of tobacco Enhancement with charcoal. Planta 155(3):281-283.
- Arditti J, Krikorian AD 1996. Orchid micropropagation: the path from laboratory to commercialization and an account of several unprecedented investigators. Botanical Journal of the Linnean Society 122(3):183-241.
- Bhatia P, Bhatia N, Ashwath N 2002. In vitro propagation of Stackhousiabtryonii Bailey (Stackhousiaceae): A rare and serpertine-endermic species of central Queensland Australia. Biodiversity Conservation 11(8):1469-1477.
- Dumas E, Monteuuis O 1995. In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants: influence of activated charcoal. Plant Cellular Tissue Organ Culture 40(3):231-235.
- Ebrahimie E, Habashy A, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B 2006. Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cellular & Developmental Biology-Plant 42(5):455-460.
- Fridborg G, Eriksson T 1975. Effects of Activated Charcoal on Growth and Morphologenesis in Cell Cultures. Physiologia Plantarum. 34(4):306-308.
- Fridborg G, Pedersen M, Eriksson T 1978. The effect of activated charcoal on tissue culture: adsorption of metabolites inhibiting morphogenesis. Physiologia Plantarum 43(2):104-106.
Ayrıntılar
Birincil Dil
İngilizce
Konular
Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm
Araştırma Makalesi
Yayımlanma Tarihi
30 Nisan 2020
Gönderilme Tarihi
30 Eylül 2019
Kabul Tarihi
8 Kasım 2019
Yayımlandığı Sayı
Yıl 2020 Cilt: 23 Sayı: 2
