TR
EN
In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)
Abstract
To investigate the regeneration potential of Lotus maritimus L., the stem, hypocotyl, cotyledon, root, and epicotyl were cultured in MS media containing different combinations of α-naphthalene acetic acid (NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0 (control), 1 mg L-1 of 6-Benzilaminopurin (BAP), and 0.5 mg L-1 of kinetin]. The highest callus induction was achieved with the stem and epicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mg L-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shoots per callus) were determined with the epicotyl and hypocotyl cultured in MS media containing 1 mg L-1 of NAA. The obtained shoots were easily rooted in half-strength MS media containing 1 mg L-1 NAA and substantially adapted to the external environment.
Keywords
Thanks
This article is based on Pelin GÖKÇE’s master thesis entitled with "Determination of in vitro regeneration ability of Lotus maritimus L."
References
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Details
Primary Language
English
Subjects
Agricultural, Veterinary and Food Sciences
Journal Section
Research Article
Publication Date
August 31, 2020
Submission Date
December 10, 2019
Acceptance Date
March 17, 2020
Published in Issue
Year 2020 Volume: 23 Number: 4
