TR
EN
In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)
Abstract
To investigate the regeneration potential of Lotus maritimus L., the stem, hypocotyl, cotyledon, root, and epicotyl were cultured in MS media containing different combinations of α-naphthalene acetic acid (NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0 (control), 1 mg L-1 of 6-Benzilaminopurin (BAP), and 0.5 mg L-1 of kinetin]. The highest callus induction was achieved with the stem and epicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mg L-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shoots per callus) were determined with the epicotyl and hypocotyl cultured in MS media containing 1 mg L-1 of NAA. The obtained shoots were easily rooted in half-strength MS media containing 1 mg L-1 NAA and substantially adapted to the external environment.
Keywords
Teşekkür
This article is based on Pelin GÖKÇE’s master thesis entitled with "Determination of in vitro regeneration ability of Lotus maritimus L."
Kaynakça
- Akashi R, Hoffmann-Tsay SS, Hoffmann F 1998. Selection of a Super-Growing Legume Root Culture that Permits Controlled Switching between Root Cloning and Direct Embryogenesis. Theoretical and Applied Genetics, 96 (6-7): 758–764. https://doi.org/10.1007/s001220050799.
- Arambarri AM, Sebastian C, Stenglein A, Colares MN, Novoa MC 2005. Taxonomy of the New World Species of Lotus (Leguminosae: Loteae). Australian Journal of Botany, 53: 797–812. DOI: 10.1071/BT04101.
- Díaz P, Borsani O, Monza J 2005. Lotus japonicus Related Species and Their Agronomic Importance. (Lotus japonicus Handbook, Springer, Netherlands: Ed. Marquez AJ, 25-27.
- Escaray FJ, Menendez AB, Gárriz A, Pieckenstain FL, Estrella MJ, Castagno LN, Carrasco P, Sanjuán J, Ruiz OA 2012. Ecological and Agronomic Importance of the Plant Genus Lotus. Its Application in Grassland Sustainability and the Amelioration of Constrained and Contaminated Soils. Plant Science, 182: 121-133. https://doi.org/10.1016/j.plantsci.2011.03.016.
- Espasandin FD, Collavino MM, Luna CV, Paz RC, Tarrago JR, Ruiz OA, Mroginski LA, Sansberro PA 2010. Agrobacterium tumefaciens-Mediated Transformation of Lotus tenuis and Regeneration of Transgenic Lines. Plant Cell Tissue Organ Culture, 102: 181–189. DOI: 10.1007/s11240-010-9720-x.
- George RAT 2012. Agricultural Seed Production. Cabi Publishing, Oxfordshire, UK. pp. 216.
- Handberg K, Stougaard J 1992. Lotus japonicus, an Autogamous, Diploid Legume Species for Classical and Molecular Genetics. The Plant Journal, 2 (4): 487-496. https://doi.org/10.1111/j.1365-313X.1992.00487.x.
- Heyn CC 1970. Tetragonolobus Scop. (Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh: Ed. Davis PH) Volume 3: 531.
Ayrıntılar
Birincil Dil
İngilizce
Konular
Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm
Araştırma Makalesi
Yayımlanma Tarihi
31 Ağustos 2020
Gönderilme Tarihi
10 Aralık 2019
Kabul Tarihi
17 Mart 2020
Yayımlandığı Sayı
Yıl 2020 Cilt: 23 Sayı: 4
