Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows

Selçuk Özdemir

doi:10.18016/ksutarimdoga.vi.661708

TR EN

Siyah Alaca ve Doğu Anadolu Kırmızısı Irkına Ait Sığırların Mycoplasma bovis ile Enfekte Sütlerinden Köken Alan Eksozomlardaki Yangı Ile Ilişkili miRNA’ların Ekpresyon Profili

Öz

Mikoplazma bovis, sığırlarda mastitis, artrit ve pnömoni gibi çeşitli klinik hastalıklarla ilişkili önemli bir ajandır. Bu çalışmada, Türkiye'de yetiştirilen Siyah Alaca (SA) ve Doğu Anadolu Kırmızısı (DAK) sığırlarına ait Mycoplasma bovis ile enfekte subklinik mastitli ve normal sütlerde inflamasyon ile ilişkili miRNA adaylarının belirlenmesi amaçlandı. Mastitli ve normal sığırlardan elde edilen sütteki miRNA'ların ekspresyon seviyeleri qRT-PCR ile analiz edildi. MiR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, miR-182'nin ekspresyon düzeylerinin her iki sığıra ait mastitli sütte arttığı gözlendi. Bununla birlikte, miR-21 ve miR-222'nin Holştayn sığırının mastitli sütünde önemli ölçüde arttığı, miR-146a ve miR-383'ün ise DAK sığırının mastitli sütünde önemli ölçüde arttığı belirlendi. Sonuç olarak, subklinik mastitli sütte ekspresyon düzeyi artan miRNA adayları Holştayn ve DAK sığırlarında belirlendi. Araştırmadan elde edilen bulgular, subklinik mastitis sütünde ekspresyon düzeyi artan miRNA'ların Mycoplasma bovis'in neden olduğu subklinik masitit tanısında biyobelirteç olarak kullanılabileceğini göstermiştir.

Anahtar Kelimeler

Mikrorna,Biomarkör,Mikoplazma Bovis,Subklinik mastitis,Süt

Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows

Abstract

Mycoplasma bovis is an important pathogen associated with several clinical diseases in cattle, such as mastitis, arthritis, and pneumonia. TableIn this study, we aimed to identify miRNA candidate biomarkers associated with inflammation in Mycoplasma bovis -infected milk samples and normal milk samples of Holstein-Friesian (HF) and Doğu Anadolu Kırmızısı (DAK) cows in Turkey. The expression levels of miRNAs in milk from mastitis-infected cows and uninfected cows were analyzed using a qRT-PCR. The results revealed that miR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, and miR-182 were upregulated in mastitis milk. Among the miRNA candidate biomarkers, miR-21 and miR-222 were significantly upregulated only in mastitis milk samples from HF cows, and miR-146a and miR-383 were significantly upregulated only in mastitis milk samples from DAK cows. These results shed light on miRNA candidate biomarkers in milk from HF and DAK cows with subclinical mastitis. The upregulated miRNAs detected in the present study could be used as biomarkers in the diagnosis of subclinical mastitis caused by Mycoplasma bovis.

Keywords

Microrna,Biomarker,Mycoplasma Bovis,Subclinic mastitis,Milk

Supporting Institution

This study was supported by the Scientific Research Projects Coordination Unit of Atatürk University

Project Number

(Project Code: TCD-2017-6381, Project ID: 6381).

Thanks

This study was supported by the Scientific Research Projects Coordination Unit of Atatürk University (Project Code: TCD-2017-6381, Project ID: 6381).

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Selçuk Özdemir ^*
0000-0001-7539-0523
Türkiye

Publication Date

June 30, 2020

Submission Date

December 19, 2019

Acceptance Date

February 6, 2020

Published in Issue

Year 2020 Volume: 23 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.661708

IZ

https://izlik.org/JA92SX76HT

Cite

RIS / Bibtex

APA

Özdemir, S. (2020). Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(3), 762-771. https://doi.org/10.18016/ksutarimdoga.vi.661708

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BMC Veterinary Research

https://doi.org/10.1186/s12917-024-04002-1