Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows

Selçuk Özdemir

doi:10.18016/ksutarimdoga.vi.661708

TR EN

Siyah Alaca ve Doğu Anadolu Kırmızısı Irkına Ait Sığırların Mycoplasma bovis ile Enfekte Sütlerinden Köken Alan Eksozomlardaki Yangı Ile Ilişkili miRNA’ların Ekpresyon Profili

Öz

Mikoplazma bovis, sığırlarda mastitis, artrit ve pnömoni gibi çeşitli klinik hastalıklarla ilişkili önemli bir ajandır. Bu çalışmada, Türkiye'de yetiştirilen Siyah Alaca (SA) ve Doğu Anadolu Kırmızısı (DAK) sığırlarına ait Mycoplasma bovis ile enfekte subklinik mastitli ve normal sütlerde inflamasyon ile ilişkili miRNA adaylarının belirlenmesi amaçlandı. Mastitli ve normal sığırlardan elde edilen sütteki miRNA'ların ekspresyon seviyeleri qRT-PCR ile analiz edildi. MiR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, miR-182'nin ekspresyon düzeylerinin her iki sığıra ait mastitli sütte arttığı gözlendi. Bununla birlikte, miR-21 ve miR-222'nin Holştayn sığırının mastitli sütünde önemli ölçüde arttığı, miR-146a ve miR-383'ün ise DAK sığırının mastitli sütünde önemli ölçüde arttığı belirlendi. Sonuç olarak, subklinik mastitli sütte ekspresyon düzeyi artan miRNA adayları Holştayn ve DAK sığırlarında belirlendi. Araştırmadan elde edilen bulgular, subklinik mastitis sütünde ekspresyon düzeyi artan miRNA'ların Mycoplasma bovis'in neden olduğu subklinik masitit tanısında biyobelirteç olarak kullanılabileceğini göstermiştir.

Anahtar Kelimeler

Mikrorna,Biomarkör,Mikoplazma Bovis,Subklinik mastitis,Süt

Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows

Abstract

Mycoplasma bovis is an important pathogen associated with several clinical diseases in cattle, such as mastitis, arthritis, and pneumonia. TableIn this study, we aimed to identify miRNA candidate biomarkers associated with inflammation in Mycoplasma bovis -infected milk samples and normal milk samples of Holstein-Friesian (HF) and Doğu Anadolu Kırmızısı (DAK) cows in Turkey. The expression levels of miRNAs in milk from mastitis-infected cows and uninfected cows were analyzed using a qRT-PCR. The results revealed that miR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, and miR-182 were upregulated in mastitis milk. Among the miRNA candidate biomarkers, miR-21 and miR-222 were significantly upregulated only in mastitis milk samples from HF cows, and miR-146a and miR-383 were significantly upregulated only in mastitis milk samples from DAK cows. These results shed light on miRNA candidate biomarkers in milk from HF and DAK cows with subclinical mastitis. The upregulated miRNAs detected in the present study could be used as biomarkers in the diagnosis of subclinical mastitis caused by Mycoplasma bovis.

Keywords

Microrna,Biomarker,Mycoplasma Bovis,Subclinic mastitis,Milk

Destekleyen Kurum

This study was supported by the Scientific Research Projects Coordination Unit of Atatürk University

Proje Numarası

(Project Code: TCD-2017-6381, Project ID: 6381).

Teşekkür

This study was supported by the Scientific Research Projects Coordination Unit of Atatürk University (Project Code: TCD-2017-6381, Project ID: 6381).

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Selçuk Özdemir ^*
0000-0001-7539-0523
Türkiye

Yayımlanma Tarihi

30 Haziran 2020

Gönderilme Tarihi

19 Aralık 2019

Kabul Tarihi

6 Şubat 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 23 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.661708

IZ

https://izlik.org/JA92SX76HT

Kaynak Göster

RIS / Bibtex

APA

Özdemir, S. (2020). Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(3), 762-771. https://doi.org/10.18016/ksutarimdoga.vi.661708