Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Dönay Parlak; Hüseyin Tanış; Ashabil Aygan

doi:10.18016/ksutarimdoga.vi.678756

EN TR

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Öz

Keratinaz aktivitesi gösteren Trichophyton sp. Tr-9 suşu keratin tozu- feather meal içeren minimal besiyerinde büyütürek enzim üretimi gerçekleştirilmiştir. Süpernatanttan Amonyum sülfat çöktürme sonrası, Sephadeks G-100 ve DEAE Sepharose kolon kromatografi uygulamaları ile enzim saflaştırılması yapılmıştır. Maksimum keratinolitik aktivite pH 7.5 ve 37°C’de gözlenmiştir. Enzim pH 5.5-8.0 ve 20°C-40°C sıcaklık aralıklarında stabilite gösterirken, enziminin moleküler ağırlığı SDS page ve zimogram analizlerinde yaklaşık 34 kDa olarak hesaplandı. CaCI2 (5mM) keratinaz aktivitesini (%148) stimüle edici etki gösterdi. Diğer taraftan EDTA (5mM) ve SDS (%1) sırasıyla %49, % 49 etki göstererek kısmen inhibe etti, PMSF (1mM ve 5mM) ile tam inaktivasyon elde edildi. Sonuç olarak, enzimin fiziko-kimyasal özellikleri birçok endüstriyel ve biyoteknolojik uygulamalarda kullanışlı olabileceğini göstermiştir.

Anahtar Kelimeler

Trichophyton sp.,Keratinaz,Saflaştırma,Karakterizasyon

Supporting Institution

K.S.Ü. BAP

Project Number

2009/6-4M

Thanks

Bu çalışma KSU Bilimsel araştırmalar birimi tarafından 2009/4-6M Nolu proje ile desteklenmiştir.

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Dönay Parlak
0000-0002-8940-5842
Türkiye

Hüseyin Tanış ^*
0000-0002-2012-7864
Türkiye

Ashabil Aygan
0000-0003-4936-9872
Türkiye

Publication Date

October 31, 2020

Submission Date

January 22, 2020

Acceptance Date

March 13, 2020

Published in Issue

Year 2020 Volume: 23 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.678756

IZ

https://izlik.org/JA35NA35ZX

Cite

RIS / Bibtex

APA

Parlak, D., Tanış, H., & Aygan, A. (2020). Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(5), 1135-1143. https://doi.org/10.18016/ksutarimdoga.vi.678756

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Abstract

Keywords

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Öz

Anahtar Kelimeler

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite