Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Dönay Parlak; Hüseyin Tanış; Ashabil Aygan

doi:10.18016/ksutarimdoga.vi.678756

EN TR

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Abstract

Keratinase enzyme production was accomplished from Trichophyton sp. Tr-9 in minmal medium containing feather meal. Enzyme was purified with Ammonium sulfate, Sephadeks G-100 and DEAE Sepharose column. Maxiumum keratinolitik activity was obtained at pH 7.5 and 37°C. Enzyme was higly stabil between pH 5.5-7.5 and 20-40oC. With the SDS-Page analysis of the enzyme, molecular weight of the enzyme was calculated as 34 kDa. CaCI2 (5mM) had a stimultory effect (148%) on enzyme. On the other hand, EDTA (5mM) and SDS (%1) inhibited enzyme acitivity up to 49% and 49%, respectively. PMSF (1- 5mM) has strongly inhibited enzyme. As a result, physico-chemical properties of the enzymeshowed that Trichophyton sp. Tr-9 could be useful in various industrial and biotechnological applications.

Keywords

Trichophyton sp.,Keratinase,Purification,Characterization

Supporting Institution

K.S.Ü. BAP

Project Number

2009/6-4M

Thanks

Bu çalışma KSU Bilimsel araştırmalar birimi tarafından 2009/4-6M Nolu proje ile desteklenmiştir.

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Dönay Parlak
0000-0002-8940-5842
Türkiye

Hüseyin Tanış ^*
0000-0002-2012-7864
Türkiye

Ashabil Aygan
0000-0003-4936-9872
Türkiye

Publication Date

October 31, 2020

Submission Date

January 22, 2020

Acceptance Date

March 13, 2020

Published in Issue

Year 1970 Volume: 23 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.678756

IZ

https://izlik.org/JA35NA35ZX

Cite

RIS / Bibtex

APA

Parlak, D., Tanış, H., & Aygan, A. (2020). Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(5), 1135-1143. https://doi.org/10.18016/ksutarimdoga.vi.678756

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Abstract

Keywords