Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Dönay Parlak; Hüseyin Tanış; Ashabil Aygan

doi:10.18016/ksutarimdoga.vi.678756

EN TR

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Abstract

Keratinase enzyme production was accomplished from Trichophyton sp. Tr-9 in minmal medium containing feather meal. Enzyme was purified with Ammonium sulfate, Sephadeks G-100 and DEAE Sepharose column. Maxiumum keratinolitik activity was obtained at pH 7.5 and 37°C. Enzyme was higly stabil between pH 5.5-7.5 and 20-40oC. With the SDS-Page analysis of the enzyme, molecular weight of the enzyme was calculated as 34 kDa. CaCI2 (5mM) had a stimultory effect (148%) on enzyme. On the other hand, EDTA (5mM) and SDS (%1) inhibited enzyme acitivity up to 49% and 49%, respectively. PMSF (1- 5mM) has strongly inhibited enzyme. As a result, physico-chemical properties of the enzymeshowed that Trichophyton sp. Tr-9 could be useful in various industrial and biotechnological applications.

Keywords

Trichophyton sp.,Keratinase,Purification,Characterization

Destekleyen Kurum

K.S.Ü. BAP

Proje Numarası

2009/6-4M

Teşekkür

Bu çalışma KSU Bilimsel araştırmalar birimi tarafından 2009/4-6M Nolu proje ile desteklenmiştir.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Dönay Parlak
0000-0002-8940-5842
Türkiye

Hüseyin Tanış ^*
0000-0002-2012-7864
Türkiye

Ashabil Aygan
0000-0003-4936-9872
Türkiye

Yayımlanma Tarihi

31 Ekim 2020

Gönderilme Tarihi

22 Ocak 2020

Kabul Tarihi

13 Mart 2020

Yayımlandığı Sayı

Yıl 1970 Cilt: 23 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.678756

IZ

https://izlik.org/JA35NA35ZX

Kaynak Göster

RIS / Bibtex

APA

Parlak, D., Tanış, H., & Aygan, A. (2020). Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(5), 1135-1143. https://doi.org/10.18016/ksutarimdoga.vi.678756

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Abstract

Keywords