The Use of Fourier-Transform Infrared Spectroscopy to Determine Potential Starch-based Prebiotics

Rafig Gurbanov; Sinem Tunçer

doi:10.18016/ksutarimdoga.vi.742250

TR EN

Nişasta Bazlı Potansiyel Prebiyotiklerin Belirlenmesi için Fourier-Transform Kızılötesi Spektroskopisinin Kullanımı

Öz

Probiyotikler, konağın homeostazını korumak için patojenlere karşı koruma, bağışıklık ve diyet bileşiklerinin metabolizması gibi birçok kritik işlevde önemli roller oynar. Dirençli nişasta (DN), vücutta sindirilemeyen ancak bağırsak mikrobiyotası tarafından sindirilebilerek, konakçıya çeşitli fizyolojik faydalar sağlayabilen bir grup metabolit üreten nişasta veya nişasta ürünüdür. Bir DN, faydalı bakterilerin büyümesini ve/veya aktivitesini seçici olarak uyarabiliyorsa prebiyotik olarak sınıflandırılabilir. Prebiyotik tapyoka nişastasındaki bakteri kaynaklı yapısal değişiklikleri araştırmak için Zayıflatılmış Toplam Yansıma-Fourier Dönüşümü Kızılötesi (ATR-FTIR) spektroskopisini kullandık. Tekil spektral bantların Temel Bileşen Analizi (PCA) ve yoğunluk analizleri, probiotik bakteri ile inkübe edilen tapyoka nişastasının polisakkarit bileşiminde kapsamlı değişiklikler meydana geldiğini göstermiştir; bakteriler ile inkübe edilen nişasta numuneleri, düzenli yapıda bir azalma ile birlikte daha amorf bir yapı kazanmıştır. Bu sonuçlar hızlı, ucuz ve zahmetsiz bir yöntem olarak, PCA ile birleştirilmiş FTIR spektroskopisinin, nişasta bazlı substratların potansiyel prebiyotik aktivitesinin analizi veya bir bakteri suşunun probiyotik potansiyelinin RS hidrolizleme kapasitesine bakılarak incelenmesi amacı ile araştırma alanında ve gıda endüstrisinde uygulama potansiyeline sahip olduğunu önermektedir.

Anahtar Kelimeler

The Use of Fourier-Transform Infrared Spectroscopy to Determine Potential Starch-based Prebiotics

Abstract

Probiotics play important roles in many crucial functions for maintaining the homeostasis of the host, such as protection against pathogens, immunity and metabolism of dietary compounds. Resistant starch (RS) is the starch or the starch products which cannot be digested through the body, but can be digested by the gut microbiota, producing a variety of metabolites which can provide a range of physiological benefits to the host. An RS can be classified as a prebiotic if it can selectively stimulate the growth and/or activity of the beneficial bacteria. To investigate the bacteria driven structural changes in the prebiotic tapioca starch, we used Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Principal Component Analysis (PCA) and intensity analyses of individual spectral bands exhibited comprehensive alterations in the polysaccharide composition of the tapioca starch incubated with the probiotic bacteria; the starch samples incubated with the bacteria gained a more amorphous structure with a decrease in the ordered structure. The results suggest that as a fast, cheap, and non-laborious method, FTIR spectroscopy coupled with PCA has a potential to be applied in the research area as well as in the food industry for the analysis of the potential prebiotic activity of starch-based substrates or the investigation of the probiotic potential of a bacterial strain through the examination of its RS hydrolyzing capacity.

Keywords

Supporting Institution

Bilecik Şeyh Edebali University, Scientific Research Fund

Project Number

2019-02.BŞEÜ.01-05

Thanks

This work was supported by Bilecik Şeyh Edebali University, Scientific Research Fund (2019-02.BŞEÜ.01-05) to Sinem Tunçer and partially supported by the Scientific and Technological Research Council Of Turkey-TUBITAK 2209A grants 1919B011903173 (supervisor Sinem Tunçer) and 1919B011800090 (supervisor Rafig Gurbanov).

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Rafig Gurbanov
0000-0002-5293-6447
Türkiye

Sinem Tunçer ^*
0000-0002-8947-8646
Türkiye

Publication Date

February 28, 2021

Submission Date

May 25, 2020

Acceptance Date

July 9, 2020

Published in Issue

Year 2021 Volume: 24 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.742250

IZ

https://izlik.org/JA69NC96MJ

Cite

RIS / Bibtex

APA

Gurbanov, R., & Tunçer, S. (2021). The Use of Fourier-Transform Infrared Spectroscopy to Determine Potential Starch-based Prebiotics. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(1), 22-30. https://doi.org/10.18016/ksutarimdoga.vi.742250

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