The Use of Fourier-Transform Infrared Spectroscopy to Determine Potential Starch-based Prebiotics

Rafig Gurbanov; Sinem Tunçer

doi:10.18016/ksutarimdoga.vi.742250

TR EN

Nişasta Bazlı Potansiyel Prebiyotiklerin Belirlenmesi için Fourier-Transform Kızılötesi Spektroskopisinin Kullanımı

Öz

Probiyotikler, konağın homeostazını korumak için patojenlere karşı koruma, bağışıklık ve diyet bileşiklerinin metabolizması gibi birçok kritik işlevde önemli roller oynar. Dirençli nişasta (DN), vücutta sindirilemeyen ancak bağırsak mikrobiyotası tarafından sindirilebilerek, konakçıya çeşitli fizyolojik faydalar sağlayabilen bir grup metabolit üreten nişasta veya nişasta ürünüdür. Bir DN, faydalı bakterilerin büyümesini ve/veya aktivitesini seçici olarak uyarabiliyorsa prebiyotik olarak sınıflandırılabilir. Prebiyotik tapyoka nişastasındaki bakteri kaynaklı yapısal değişiklikleri araştırmak için Zayıflatılmış Toplam Yansıma-Fourier Dönüşümü Kızılötesi (ATR-FTIR) spektroskopisini kullandık. Tekil spektral bantların Temel Bileşen Analizi (PCA) ve yoğunluk analizleri, probiotik bakteri ile inkübe edilen tapyoka nişastasının polisakkarit bileşiminde kapsamlı değişiklikler meydana geldiğini göstermiştir; bakteriler ile inkübe edilen nişasta numuneleri, düzenli yapıda bir azalma ile birlikte daha amorf bir yapı kazanmıştır. Bu sonuçlar hızlı, ucuz ve zahmetsiz bir yöntem olarak, PCA ile birleştirilmiş FTIR spektroskopisinin, nişasta bazlı substratların potansiyel prebiyotik aktivitesinin analizi veya bir bakteri suşunun probiyotik potansiyelinin RS hidrolizleme kapasitesine bakılarak incelenmesi amacı ile araştırma alanında ve gıda endüstrisinde uygulama potansiyeline sahip olduğunu önermektedir.

Anahtar Kelimeler

The Use of Fourier-Transform Infrared Spectroscopy to Determine Potential Starch-based Prebiotics

Abstract

Probiotics play important roles in many crucial functions for maintaining the homeostasis of the host, such as protection against pathogens, immunity and metabolism of dietary compounds. Resistant starch (RS) is the starch or the starch products which cannot be digested through the body, but can be digested by the gut microbiota, producing a variety of metabolites which can provide a range of physiological benefits to the host. An RS can be classified as a prebiotic if it can selectively stimulate the growth and/or activity of the beneficial bacteria. To investigate the bacteria driven structural changes in the prebiotic tapioca starch, we used Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Principal Component Analysis (PCA) and intensity analyses of individual spectral bands exhibited comprehensive alterations in the polysaccharide composition of the tapioca starch incubated with the probiotic bacteria; the starch samples incubated with the bacteria gained a more amorphous structure with a decrease in the ordered structure. The results suggest that as a fast, cheap, and non-laborious method, FTIR spectroscopy coupled with PCA has a potential to be applied in the research area as well as in the food industry for the analysis of the potential prebiotic activity of starch-based substrates or the investigation of the probiotic potential of a bacterial strain through the examination of its RS hydrolyzing capacity.

Keywords

Destekleyen Kurum

Bilecik Şeyh Edebali University, Scientific Research Fund

Proje Numarası

2019-02.BŞEÜ.01-05

Teşekkür

This work was supported by Bilecik Şeyh Edebali University, Scientific Research Fund (2019-02.BŞEÜ.01-05) to Sinem Tunçer and partially supported by the Scientific and Technological Research Council Of Turkey-TUBITAK 2209A grants 1919B011903173 (supervisor Sinem Tunçer) and 1919B011800090 (supervisor Rafig Gurbanov).

Kaynakça

Al-Tamimi EK, Seib PA, Snyder BS, Haub MD 2010. Consumption of cross-linked resistant starch (RS4XL) on glucose and insulin responses in humans. J Nutr Metab, 651063.
Alfa MJ, Strang D, Tappia PS, Olson N, DeGagne P, Bray D, Murray BL, Hiebert, B 2018. A randomized placebo controlled clinical trial to determine the impact of digestion resistant starch MSPrebiotic® on glucose, insulin, and insulin resistance in elderly and mid-age adults. Front Med, 4: 260.
Aquino JS, Batista KS, Francisca Menezes NDD, Lins PP, Gomes JAS, Silva LA 2017. Models to Evaluate the Prebiotic Potential of Foods (Functional Food - Improve Health through Adequate Food, InTech Press, UK: Ed. Chávarri M) 235-256.
Arshad NH, Zaman SA, Rawi MH, Sarbini SR 2018. Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment. Int Food Res J, 25(3): 951-957.
Birt DF, Boylston T, Hendrich S, Jane JL, Hollis J, Li L, McClelland J, Moore S, Phillips GJ, Rowling M, Schalinske K, Scott MP, Whitley EM 2013. Resistant Starch: Promise for Improving Human Health. Adv Nutr, 4(6): 587-601.
Burton JP, Drummond BK, Chilcott CN, Tagg JR, Thomson WM, Hale JDF, Wescombe PA 2013. Influence of the probiotic Streptococcus salivarius strain M18 on indices of dental health in children: A randomized double-blind, placebo-controlled trial. J Med Microbiol, 62: 875-884.
Capron I, Robert P, Colonna P, Brogly M, Planchot V 2007. Starch in rubbery and glassy states by FTIR spectroscopy. Carbohydr Polym, 68(2): 249-259.
Cebeci E, Samanci NS, Gursu M, Ozturk S, Pehlivanoglu F, Sumnu A, Karadag S, Kazancioglu R 2015. A Rare Case of Peritonitis: Streptococcus Salivarius. BANTAO J, 13(2): 95–96.

Cˇerna´ M, Barrosb AS, Nunesb A, Rochab SM, Delgadillob I, Cˇopı´kova´ J, Coimbrab MA 2003. Use of FT-IR spectroscopy as a tool for the analysis of polysaccharide food additives. Carbohydrate Polymers, 51(4): 383-389.
Cummings JH, Stephen AM 2007. Carbohydrate terminology and classification. Eur J Clin Nutr, 61: S5–S18. DeVries JW 2004. Dietary fiber: The influence of definition on analysis and regulation. J AOAC Int, 87(3): 682-706.
Di Pierro F, Zanvit A, Nobili P, Risso P, Carlo Fornaini C 2015. Cariogram outcome after 90 days of oral treatment with Streptococcus salivarius M18 in children at high risk for dental caries: Results of a randomized, controlled study. Clin Cosmet Investig Dent, 7: 107-113.
Dinkçi N, Akdeniz V, Akalin AS 2019. Survival of probiotics in functional foods during shelf life. (Food Quality and Shelf Life, Academic Press, UK: Ed. Galanakis CM) 201-233.
Erickson J, Carlson J, Stewart M, Slavin J 2018. Fermentability of Novel Type-4 Resistant Starches in In Vitro System. Foods, 7(2): 18.
Escuderos ME, Sánchez S, Jiménez A 2010. Virgin olive oil sensory evaluation by an artificial olfactory system, based on Quartz Crystal Microbalance (QCM) sensors. Sensors Actuators B Chem, 147(1): 159-164.
Giuberti G, Gallo A 2020. In vitro evaluation of fermentation characteristics of type 3 resistant starch. Heliyon, 6(1): e03145.
Gurbanov R, Yıldız F 2017. Molecular Profile Of Oral Probiotic Bacteria To Be Used With Functional Foods. J Food Health Sci, 3(4): 117-131.
Gurbanov R, S. Ozek N, Tunçer S, Severcan F, Gozen AG 2018. Aspects of silver tolerance in bacteria: infrared spectral changes and epigenetic clues. J Biophotonics, 11(5): e201700252.
Gurbanov R, Tunçer S, Mingu S, Severcan F, Gozen AG 2019. Methylation, sugar puckering and Z-form status of DNA from a heavy metal-acclimated freshwater Gordonia sp. J Photochem Photobiol B Biol, 198: 111580.
Gurbanov R, Karadağ H, Karaçam S, Samgane G 2020. Tapioca Starch Modulates Cellular Events in Oral Probiotic Streptococcus salivarius Strains. Probiotics & Antimicro. Prot. https://doi.org/10. 1007/ s12602-020-09678-z
Hale JD, Tagg JR, Wescombe PA 2012. BLIS-producing probiotics targeting the oral cavity. Microbiol Aust, 33(3): 103-105.
Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, Morelli L, Canani RB, Flint HJ, Salminen S, Calder PC, Sanders ME 2014. Expert consensus document: The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol, 11(8): 506–514.
Kasote DM, Oak MD, Nilegaonkar SS, Agte VV 2018. Effect of soaking and autoclaving treatments on structure, properties and resistant starch (RS3) content of edible tapioca pearls. Int Food Res J, 25(1): 1-8.
Koliarakis I, Messaritakis I, Nikolouzakis TK, Hamilos G, Souglakos J, Tsiaoussis J 2019. Oral bacteria and intestinal dysbiosis in colorectal cancer. Int J Mol Sci, 20(17): 4146.
Li Q, Niu Y, Xing P, Wang C 2018. Bioactive polysaccharides from natural resources including Chinese medicinal herbs on tissue repair. Chinese Med, 13:7.
Ma Z, Boye JI 2018. Research advances on structural characterization of resistant starch and its structure-physiological function relationship: A review. Crit Rev Food Sci Nutr, 58(7):1059-1083.
Mandalari G, Nueno Palop C, Tuohy K, Gibson GR, Bennett RN, Waldron KW, Bisignano G, Narbad A, Faulds CB 2007. In vitro evaluation of the prebiotic activity of a pectic oligosaccharide-rich extract enzymatically derived from bergamot peel. Appl Microbiol Biotechnol, 73(5):1173-1179.
Martin MZ, Labbe N, Wagner RJ 2014. Applications of High Resolution Laser: Induced Breakdown Spectroscopy for Environmental and Biological Samples. Springer Series in Optical Sciences, 182:439-456.
Navarro DMDL, Abelilla JJ, Stein HH 2019. Structures and characteristics of carbohydrates in diets fed to pigs: A review. J. Anim. Sci. Biotechnol, 10:39.
Ogbo FC, Okafor EN 2015. The resistant starch content of some cassava based Nigerian foods. Niger Food J, 33 (2015): 29-34.
Önal Darilmaz D, Sönmez Ş, Beyatli Y 2019. The effects of inulin as a prebiotic supplement and the synbiotic interactions of probiotics to improve oxalate degrading activity. Int J Food Sci Technol, 54(1): 121-131.
Parkar SG, Redgate EL, Wibisono R, Luo X, Koh ETH, Schröderet R 2010. Gut health benefits of kiwifruit pectins: Comparison with commercial functional polysaccharides. J Funct Foods, 2 (3): 210-218.
Pereira BLB, Leonel M 2014. Resistant starch in cassava products. Food Sci Technol, 34:298-302. Poorni S, Srinivasan M, Nivedhitha M 2019. Probiotic Streptococcus strains in caries prevention: A systematic review. J. Conserv. Dent, 22(2): 123-128.
Ratnayake WS, Jackson DS 2008. Thermal Behavior of Resistant Starches RS 2, RS 3, and RS 4. J Food Sci, 73:C356-C366.
Riquelme SA, Ahn D, Prince A 2018. Pseudomonas aeruginosa and Klebsiella pneumoniae Adaptation to Innate Immune Clearance Mechanisms in the Lung. J. Innate Immun, 10(5-6):442-454.
Rodríguez-Cabezas ME, Camuesco D, Arribas B, Garrido-Mesa N, Comalada M, Bailón E, Cueto-Sola M, Utrilla P, Guerra-Hernández E, Pérez-Roca C, Gálvez J, Zarzuelo A 2010. The combination of fructooligosaccharides and resistant starch shows prebiotic additive effects in rats. Clin Nutr, 29(6):832-839.
Sanden KW, Kohler A, Afseth NK, Böcker U, Rønning SB, Liland KH, Pedersen ME 2019. The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.). J Biophotonics, 12(9):e201800436.
Saulnier DMA, Spinler JK, Gibson GR, Versalovic J 2009. Mechanisms of Probiosis and Prebiosis: Considerations for Enhanced Functional Foods. Curr Opin Biotechnol, 20(2): 135-141.
Stewart ML, Zimmer JP 2017. A high fiber cookie made with resistant starch type 4 reduces post-prandial glucose and insulin responses in healthy adults. Nutrients, 9(3):237.
Thomas P, Sekhar AC, Upreti R, Mujawar MM, Pasha SS 2015. Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples. Biotechnol Rep (Amst), 8: 45-55.
Tunçer S, Gurbanov G 2020. A novel approach for the discrimination of culture medium from Vascular Endothelial Growth Factor (VEGF) overexpressing colorectal cancer cells. Turkish Journal of Biochemistry, 20200058, eISSN 1303-829X, ISSN 0250-4685, DOI: https://doi.org/10.1515/tjb-2020-0058.
Tunçer S, Karaçam S 2020. Cell-free supernatant of Streptococcus salivarius M18 impairs the pathogenic properties of Pseudomonas aeruginosa and Klebsiella pneumonia. Arch Microbiol, 202(10):2825-2840.
Van Soest JJG, Tournois H, de Wit D, Vliegenthart JFG 1995. Short-range structure in (partially) crystalline potato starch determined with attenuated total reflectance Fourier-transform IR spectroscopy. Carbohydr Res, 279: 201-214.
Vulevic J, Rastall RA, Gibson GR 2004. Developing a quantitative approach for determining the in vitro prebiotic potential of dietary oligosaccharides. FEMS Microbiol Lett, 236(1):153-159.
Wang S, Wang J, Zhang W, Li C, Yu J, Wang S 2015. Molecular order and functional properties of starches from three waxy wheat varieties grown in China. Food Chem, 181:43-50.
Wescombe PA, Hale JD, Heng NC, Tagg JR 2012. Developing oral probiotics from Streptococcus salivarius. Future Microbiol, 7(12):1355-1371.
Wronkowska M, Soral-Śmietana M, Biedrzycka E 2008 Utilization of resistant starch of native tapioca, corn and waxy corn starches and their retrograded preparations by Bifidobacterium. Int J Food Sci Nutr, 59:80-87.
Zaman SA, Sarbini SR 2016. The potential of resistant starch as a prebiotic. Crit. Rev. Biotechnol, 36(3):578-584.
Zhang Y, Zeng H, Wang Y, Zeng S, Zheng B 2014. Structural characteristics and crystalline properties of lotus seed resistant starch and its prebiotic effects. Food Chem, 155:311-318.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Rafig Gurbanov
0000-0002-5293-6447
Türkiye

Sinem Tunçer ^*
0000-0002-8947-8646
Türkiye

Yayımlanma Tarihi

28 Şubat 2021

Gönderilme Tarihi

25 Mayıs 2020

Kabul Tarihi

9 Temmuz 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.742250

IZ

https://izlik.org/JA69NC96MJ

Kaynak Göster

RIS / Bibtex

APA

Gurbanov, R., & Tunçer, S. (2021). The Use of Fourier-Transform Infrared Spectroscopy to Determine Potential Starch-based Prebiotics. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(1), 22-30. https://doi.org/10.18016/ksutarimdoga.vi.742250