The Antioxidant Effect of p-Coumaric Acid Against Toluene-Induced Oxidative Stress in Rats

Zeki Erol; Selinay Başak Erdemli Köse; Simge Garlı; Fatma Kocasarı

doi:10.18016/ksutarimdoga.vi.765193

TR EN

Ratlarda Toluenle İndüklenen Oksidatif Hasara Karşı p-Kumarik Asitin Antioksidan Etkisi

Öz

TBu araştırmada, fenolik asidin bir türevi olan p-CA'nın toluen kaynaklı oksidatif hasara karşı koruyucu etkisinin araştırılması amaçlanmıştır. Çalışmada, her grupta 8 olmak üzere toplam 32 Sprague-Dawley erkek sıçan kullanıldı. Kontrol, toluen, p-CA ve toluen+p-CA olarak toplam 4 grup oluşturuldu. Kontrol grubu, toluen grubu ve p-CA grubundaki hayvanlara 21 gün boyunca sırasıyla %0.9 NaCl, 0.9 mg kg-1 c.a toluen ve 100 mg kg-1 c.a p-CA verildi. Toluen+p-CA grubundaki hayvanlara 3 gün boyunca p-CA verildi ve 4. günden itibaren toluen ve p-CA 25 güne kadar günlük olarak birlikte uygulandı. 25. günde çalışma sonlandırıldı ve kan örnekleri alındı. Eritrositlerde katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) aktiviteleri ile malondialdehit (MDA) ve glutatyon (GSH) seviyeleri; plazma örneklerinde süperoksit dismutaz (SOD) aktivitesi belirlendi. Bu çalışmada, toluen grubunun kan örneklerinde CAT ve SOD aktiviteleri ve MDA düzeyi anlamlı (p <0.05) olarak arttığı; GSH düzeyi ve GSH-Px aktivitesinin anlamlı (p <0.05) olarak azaldığı tespit edildi. Toluen+p-CA grubunda, toluen grubuna göre GSH düzeyi ve GSH-Px aktivitesinde anlamlı (p <0.05) artış; MDA düzeyi ile ve SOD ve CAT aktivitelerinde anlamlı (p <0.05) azalma saptandı. Toluenin kan dokusunda oksidatif strese ve lipit peroksidasyonuna neden olduğu ve bu oksidatif hasara karşı p-CA'nın koruyucu bir etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

The Antioxidant Effect of p-Coumaric Acid Against Toluene-Induced Oxidative Stress in Rats

Abstract

This research aimed to investigate the protective effect of p-CA, a derivative of phenolic acid, against toluene-induced oxidative damage. A total of 32 Sprague-Dawley male rats, 8 in each group, were used. A total of 4 groups were formed as control, toluene, p-CA and toluene+p-CA. Animals in the control group, toluene group and p-CA group were given 0.9 % NaCl, 0.9 mg kg-1 b.w toluene and 100 mg kg-1 b.w p-CA orally for 21 days, respectively. The animals in toluene+p-CA group were received p-CA for 3 days and from day 4, toluene and p-CA were applied together daily until day 25. On the 25th day, the study was terminated, and blood samples were collected. Catalase (CAT) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) and glutathione (GSH) levels in the erythrocytes and superoxide dismutase (SOD) activity in plasma samples were determined. In this study, CAT and SOD activities and MDA level significantly (p <0.05) increased, GSH level and GSH-Px activity significantly (p <0.05) decreased in the blood samples of toluene group compared to the control group. In toluene+p-CA group, significant (p <0.05) increases in GSH level and GSH-Px activity and significant (p <0.05) decreases in MDA level and SOD and CAT activities were detected compared to the toluene group. It has been determined that toluene caused oxidative stress and lipid peroxidation in blood tissue and against this oxidative damage, p-CA had a protective effect.

Keywords

Supporting Institution

TUBİTAK

Project Number

1919BO11501737

Thanks

Authors thank TUBITAK for supporting this project.

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Zeki Erol
0000-0002-1563-0043
Türkiye

Selinay Başak Erdemli Köse
0000-0001-8986-585X
Türkiye

Simge Garlı
0000-0002-9818-5212
Türkiye

Fatma Kocasarı ^*
0000-0002-6123-4762
Türkiye

Publication Date

June 30, 2021

Submission Date

July 6, 2020

Acceptance Date

September 16, 2020

Published in Issue

Year 2021 Volume: 24 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.765193

IZ

https://izlik.org/JA77LL25NZ

Cite

RIS / Bibtex

APA

Erol, Z., Erdemli Köse, S. B., Garlı, S., & Kocasarı, F. (2021). The Antioxidant Effect of p-Coumaric Acid Against Toluene-Induced Oxidative Stress in Rats. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(3), 473-478. https://doi.org/10.18016/ksutarimdoga.vi.765193

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The Antioxidant Effect of p-Coumaric Acid Against Toluene-Induced Oxidative Stress in Rats

Ratlarda Toluenle İndüklenen Oksidatif Hasara Karşı p-Kumarik Asitin Antioksidan Etkisi

Öz

Anahtar Kelimeler

The Antioxidant Effect of p-Coumaric Acid Against Toluene-Induced Oxidative Stress in Rats

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

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Alginate nanoparticles loaded with enriched polyphenols of Desmodium gangeticum alleviates diabetes induced oxidative and inflammatory stress in Rin-5F cells via GLUT2/IRS-1/NF-κB pathway

The mitigating effects of Tribulus terrestris extract on the cytotoxic, genotoxic, biochemical and phytotoxic effects of omethoate in roots of Allium cepa bulbs