Antioxidant Activity and Phenolic Composition of Ethanol Extracts of Momordica charantia and Datura stramonium

Mehlika Alper; Cennet Özay

doi:10.18016/ksutarimdoga.vi.851251

TR EN

Momordica charantia ve Datura stramonium Etanol Ekstraktlarının Antioksidan Aktivitesi ve Fenolik Kompozisyonu

Öz

Dünyadaki tıbbi bitkiler, fenolik asitler ve flavonoidler gibi bazı sekonder metabolitler içerdikleri için doğal antioksidan kaynaklarıdır. Bu nedenle dünyanın pek çok yerinde birçok hastalığı önlemek veya tedavi etmek için kullanılmaktadırlar. Bununla birlikte, antioksidanların kanser tedavisinde kullanımı hala tartışmalıdır. Bu doğrultuda, Türkiye'de geleneksel olarak kullanılan ve insan kanser hücreleri üzerinde sitotoksik potansiyele sahip Momordica charantia L. (Cucurbitaceae) ve Datura stramonium L. (Solanaceae) 'un etanol ekstraktlarının fenolik bileşimi ve antioksidan potansiyeli araştırılmıştır. Antioksidan deneyler (DPPH, metal şelatlama, fosfomolibdenyum ve demir indirgeme gücü), M. charantia'nın meyvelerinin ve D. stramonium'un yapraklarının etanolik ekstraktlarına uygulandı. Toplam fenolik ve flavonoid içerikler belirlendi. Etanolik ekstraktlarda bulunan fenolik bileşikler HPLC yöntemi kullanılarak tespit edildi. Ekstraktlarda on beş fenolik bileşik tanımlandı. Kafeik asit, her iki ekstraktta da ana bileşendi. M. charantia ekstraktı ve D. stramonium ekstraktında kafeik asit miktarları sırasıyla 6282.51 ve 15183.36 µg g-1 olarak tespit edildi. D. stramonium yaprak ekstraktı, M. charantia meyve ekstraktından daha yüksek antioksidan aktivite gösterdi ve bu aktivite yüksek fenolik bileşik konsantrasyonları ile bağlantılı olabilir. Bu nedenle, bu bitkilerin çeşitli kısımlarından biyoaktif bileşiklerin taranması ile ilgili daha ileri çalışmalar, yeni doğal antioksidan kaynaklarının elde edilmesi için büyük önem taşıyacaktır.

Anahtar Kelimeler

Antioxidant Activity and Phenolic Composition of Ethanol Extracts of Momordica charantia and Datura stramonium

Abstract

Medicinal plants in the world are natural antioxidant sources as they contain some secondary metabolites such as phenolic acids and flavonoids. Therefore, they are used to prevent or treat many diseases in many parts of the world. However, the use of antioxidants in cancer treatment is still controversial. Accordingly, the phenolic composition and antioxidant potential of the ethanol extracts of Momordica charantia L. (Cucurbitaceae) and Datura stramonium L. (Solanaceae), which are used traditionally in Turkey and have cytotoxic potential on human cancer cells, were investigated. The antioxidant assays (DPPH, metal chelating, phosphomolybdenum, and ferric reducing power) were applied to the ethanol extracts of the fruits of M. charantia and the leaves of D. stramonium. The total phenolic and flavonoid contents were determined. The phenolic compounds contained in ethanolic extracts were identified using HPLC method. Fifteen phenolic compounds were identified in the extracts. Caffeic acid was the major constituent in both extracts. The amount of caffeic acid was detected to be 6282.51 and 15183.36 µg g-1 extract in M. charantia extract and the D. stramonium extracts, respectively. D. stramonium leaf extract showed higher antioxidant activity than M. charantia fruit extract and this activity may be associated with high phenolic compound concentrations. Hence, further studies about screening of bioactive compounds from various part of these plants will be a great importance for obtaining of source of novel natural antioxidants.

Keywords

References

Alam MdN, Bristi NJ, Rafiquzzaman Md 2013. Review on In Vivo and In Vitro Methods Evaluation of Antioxidant Activity. Saudi Pharm J 21(2): 143-152.
Ally F, Mohanlall, V 2020. An Overview of Tropane Alkaloids from Datura stramonium L.. J Pharmacogn Phytochem 9(3): 5-13.
Alper M, Özay C, Güneş H, Mammadov R 2021. Assessment of Antioxidant and Cytotoxic Activities and Identification of Phenolic Compounds of Centaurea solstitialis and Urospermum picroides from Turkey. Braz Arch Biol Technol 64: e21190530.
Alper M 2019. Investigation of Potential Anti-cancer and Anti-inflammatory Effects of Datura stramonium Ethanolic Extracts Against Selected Human Cancer Cell Lines. Fresen Environ Bull 28(12): 8993-9003.
Al-Snafi AE 2017. Medical Importance of Datura fastuosa (syn: Datura metel) and Datura stramonium - A Review. IOSR J Pharm 7(2): 43-58.
Armendáriz-Barragán B, Zafar N, Badri W, Galindo-Rodríguez SA, Kabbaj D, Fessi H, Elaissari H 2016. Plant Extracts: From Encapsulation to Application. Expert Opin Drug Del 13(8): 1165-1175.
Benabderrahim MA, Sarikurkcu C, Elfalleh W, Özer MS 2019. Datura innoxia and Dipsacus laciniatus: Biological Activity and Phenolic Composition. Biocatal Agric Biotechnol 19: 101163.
Berk S, Tepe B, Arslan S, Sarikurkcu C 2011. Screening of The Antioxidant, Antimicrobial and DNA Damage Protection Potentials of The Aqueous Extract of Asplenium ceterach DC. Afr J Biotechnol 10(44): 8902-8908.

Berkov S, Zayed R, Doncheva T 2006. Alkaloid Patterns in Some Varieties of Datura stramonium. Fitoterapia 77(3): 179-182.
Bhardwaj K, Kumar S, Ojha S 2016. Antioxidant Activity and FT-IR Analysis of Datura innoxia and Datura metel Leaf and Seed Methanolic Extracts. Afr J Tradit Complement Altern Med 13(5): 7-16.
Cai YZ, Sun M, Xing J, Luo Q, Corke H 2006. Structure-Radical Scavenging Activity Relationships of Phenolic Compounds from Traditional Chinese Medicinal Plants. Life Sci 78(25): 2872-2888.
Caponio F, Alloggio V, Gomes T 1999. Phenolic Compounds of Virgin Olive Oil: Influence of Paste Preparation Techniques. Food Chem 64(2): 203-209.
Carocho M, Ferreira ICFR 2013. A Review on Antioxidants, Prooxidants and Related Controversy: Natural and Synthetic Compounds, Screening and Analysis Methodologies and Future Perspectives. Food Chem Toxicol 51: 15-25.
Ceylan R, Katanić J, Zengin G, Matić S, Aktumsek A, Boroja T, Stanić S, Mihailović V, Guler GO, Boga M, Yılmaz MA 2016. Chemical and Biological Fingerprints of Two Fabaceae Species (Cytisopsis dorycniifolia and Ebenus hirsuta): Are They Novel Sources of Natural Agents for Pharmaceutical and Food Formulations? Ind Crop Prod 84: 254-262.
Chen F, Huang G, Yang Z, Hou Y 2019 Antioxidant Activity of Momordica charantia Polysaccharide and Its Derivatives. Int J Biol Macromol 138: 673-680.
Choi JS, Kim HY, Seo WT, Lee JH, Cho KM 2012. Roasting Enhances Antioxidant Effect of Bitter Melon (Momordica charantia L.) Iincreasing Inflavan-3-ol and Phenolic Acid Contents. Food Sci Biotechnol 21(1): 19-26.
Cuong DM, Kwon SJ, Jeon J, Park YJ, Park JS, Park SU 2018. Identification and Characterization of Phenylpropanoid Biosynthetic Genes and Their Accumulation in Bitter Melon (Momordica charantia). Molecules 23(2): 469.
Dai J, Mumper RJ 2010. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules 15(10): 7313-7352.
Dastmalchi N, Baradaran B, Latifi-Navid S, Safaralizadeh R, Khojasteh SMB, Amini M, Roshani E, Lotfinejad P 2020. Antioxidants with Two Faces toward Cancer. Life Sci 258: 118186.
El-Hawary SS, El-Hefnawy HM, Osman SM, El-Raey MA, Mokhtar Ali FA 2019. Phenolic Profiling of Different Jasminum Species Cultivated in Egypt and Their Antioxidant Activity. Nat Prod Res 14: 1-6.
El Bazaoui A, Bellimam, MA, Soulaymani A 2011. Nine New Tropane Alkaloids from Datura stramonium L. Identified by GC/M. Fitoterapia 82(2): 193-197.
Fatima H, Khan K, Zia M, Ur-Rehman T, Mirza B, Haqi IU 2015. Extraction Optimization of Medicinally Important Metabolites from Datura innoxia Mill.: An In Vitro Biological and Phytochemical Investigation. BMC Complement Altern Med 15: 376.
Fuchs-Tarlovsky V 2013. Role of Antioxidants in Cancer Therapy. Nutrition 29(1): 15-21.
Ghaima KK, Nader MI, Taqi RA, Ghraibit SA 2013. Extraction and Identification of Phenol Compounds from Bitter Melon Momordica charantia Fruits and Their Role as Antioxidants. J Biotech Res Cent 7(1): 41-47.
Güneş H, Alper M, Çelikoğlu N 2019. Anticancer Effect of The Fruit and Seed Extracts of Momordica charantia L. (Cucurbitaceae) on Human Cancer Cell Lines. Trop J Pharm Res 18(10): 2057-2065.
Horax R, Hettiarachchy N, Chen P 2010. Extraction, Quantification, and Antioxidant Activities of Phenolics from Pericarp and Seeds of Bitter Melons (Momordica charantia) Harvested at Three Maturity Stages (Immature, Mature, and Ripe). J Agric Food Chem 58(7): 4428-4433.
Krishnaiah D, Sarbatly R, Nithyanandam R 2011. A Review of The Antioxidant Potential of Medicinal Plant Species. Food Bioprod Process 89: 217-233.
Kubola J, Siriamornpun S 2008. Phenolic Contents and Antioxidant Activities of Bitter Gourd (Momordica charantia L.) Leaf, Stem and Fruit Fraction Extracts In Vitro. Food Chem 110: 881-890.
Lee SH, Jeong YS, Song J, Hwang KA, Noh GM, Hwang IG 2017. Phenolic Acid, Carotenoid Composition, and Antioxidant Activity of Bitter Melon (Momordica charantia L.) at Different Maturation Stages. Int J Food Prop 20: sup3, S3078-S3087.
Lobo V, Patil A, Phatak A, Chandra N 2010. Free Radicals, Antioxidants and Functional Foods: Impact on Human Health. Phcog Rev 4: 118-126.
López-Alarcón C, Denicola A 2013. Evaluating The Antioxidant Capacity of Natural Products: A Review on Chemical and Cellular-Based Assays. Anal Chim Acta 763: 1-10.
Masek A, Chrzescijanska E, Latos M 2016. Determination of Antioxidant Activity of Caffeic Acid and p-Coumaric Acid by Using Electrochemical and Spectrophotometric Assays. Int J Electrochem Sci 11: 10644-10658.
Ozay C, Mammadov R 2016. Assessment of Some Biological Activities of Alyssum L. Known as Madwort. Acta Pol Pharm 73(5): 1213-1220.
Ozusaglam MA, Karakoca K 2013. Antimicrobial and Antioxidant Activities of Momordica charantia from Turkey. Afr J Biotechnol 12: 1548-1558.
Öz Arık U 2017. The Antifungal Effects of Datura stramonium L., D. metel L., D. innoxia Mill. in Flora of Turkey. Mugla J Sci Technol 3(2): 96-103.
Padmashree A, Sharma GK, Semwal AD, Bawa AS 2010. Studies on Antioxygenic Activity of Bitter Gourd (Momordica charantia) and Its Fractions Using Various In Vitro Models. J Sci Food Agric 91: 776-782.
Partap M, Gupta RC, Pradhan SK 2019. Comparative Analysis of Morphology and Phytochemical Constituents in Different Populations and Morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab Plains. Asian J Pharm Clin Res 12(1): 193-199.
Rahmoune B, Zerrouk IZ, Morsli A, Slaoui MK, Khelifi L, Do Amarante L 2017. Phenylpropanoids and Fatty Acids Levels in Roots and Leaves of Datura stramonium and Datura innoxia. Int J Pharm Pharm Sci 9(7): 150-154.
Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, Gopas J, Nishigaki I 2014. Antioxidants and Human Diseases. Clin Chim Acta 436: 332-347.
Raza A, Kamran M, Safdar ME, Ali HH, Abbas T, Asif M, Ali L, Rehman A 2019. Management Tactics for The Handling of Parthenium hysterophorus L. in Non-Native Environment Through Phytotoxic Compounds of Local Species. Int J Agric Biol 21(1): 215-222.
Singh LR, Singh OM 2013. Datura stramonium: An Overview of Its Phytochemistry and Pharmacognosy. Res J Pharmacogn Phytochem 5(3): 143-148.
Soni P, Siddiqui AA, Dwivedi J, Soni V 2012. Pharmacological Properties of Datura stramonium L. as A Potential Medicinal Tree: An Overview. Asian Pac J Trop Biomed 2(12): 1002-1008.
SPSS 2013. IBM SPSS Statistics 22.0 for Windows. Armonk, NY
Sreenivasa S, Vinay K, Mohan NR 2012. Phytochemical Analysis, Antibacterial and Antioxidant Activity of Leaf Extract of Datura stramonium. Int J Sci Res 1(2): 83-86.
Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A 2018. Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines 5(93): 1-16.
Verma N, Shukla S 2015. Impact of Various Factors Responsible for Fluctuation in Plant Secondary Metabolites. J Appl Res Med Aromat Plants 2(4): 105-113.
Villarreal-La Torre VE, Sagástegui Guarniz W, Silva-Correa C, Cruzado-Razco L, Siche R 2020. Antimicrobial Activity and Chemical Composition of Momordica Charantia: A Review. Pharmacog J 12(1): 213-22.
Wu SJ, Ng LT 2008. Antioxidant and Free Radical Scavenging Activities of Wild Bitter Melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT-Food Sci Technol 41: 323-330.
Zengin G, Aktumsek A 2014. Investigation of Antioxidant Potentials of Solvent Extracts from Different Anatomical Parts of Asphodeline anatolica E. Tuzlaci: An Endemic Plant to Turkey. Afr J Tradit Complement Altern Med 11(2): 481-488.
Zengin G, Nithiyanantham S, Locatelli, M, Ceylan R, Uysal S, Aktumsek A, Selvi PK, Maskovic P 2016. Screening of In Vitro Antioxidant and enzyme Inhibitory Activities of Different Extracts from Two Uninvestigated Wild Plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata. Eur J Integr Med 8(3): 286-292.

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Mehlika Alper ^*
0000-0001-6193-346X
Türkiye

Cennet Özay
0000-0002-1120-6122
Türkiye

Publication Date

February 28, 2022

Submission Date

December 31, 2020

Acceptance Date

April 9, 2021

Published in Issue

Year 2022 Volume: 25 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.851251

IZ

https://izlik.org/JA77YR35TZ

Cite

RIS / Bibtex

APA

Alper, M., & Özay, C. (2022). Antioxidant Activity and Phenolic Composition of Ethanol Extracts of Momordica charantia and Datura stramonium. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(1), 1-9. https://doi.org/10.18016/ksutarimdoga.vi.851251