Antioxidant Activity and Phenolic Composition of Ethanol Extracts of Momordica charantia and Datura stramonium

Mehlika Alper; Cennet Özay

doi:10.18016/ksutarimdoga.vi.851251

TR EN

Momordica charantia ve Datura stramonium Etanol Ekstraktlarının Antioksidan Aktivitesi ve Fenolik Kompozisyonu

Öz

Dünyadaki tıbbi bitkiler, fenolik asitler ve flavonoidler gibi bazı sekonder metabolitler içerdikleri için doğal antioksidan kaynaklarıdır. Bu nedenle dünyanın pek çok yerinde birçok hastalığı önlemek veya tedavi etmek için kullanılmaktadırlar. Bununla birlikte, antioksidanların kanser tedavisinde kullanımı hala tartışmalıdır. Bu doğrultuda, Türkiye'de geleneksel olarak kullanılan ve insan kanser hücreleri üzerinde sitotoksik potansiyele sahip Momordica charantia L. (Cucurbitaceae) ve Datura stramonium L. (Solanaceae) 'un etanol ekstraktlarının fenolik bileşimi ve antioksidan potansiyeli araştırılmıştır. Antioksidan deneyler (DPPH, metal şelatlama, fosfomolibdenyum ve demir indirgeme gücü), M. charantia'nın meyvelerinin ve D. stramonium'un yapraklarının etanolik ekstraktlarına uygulandı. Toplam fenolik ve flavonoid içerikler belirlendi. Etanolik ekstraktlarda bulunan fenolik bileşikler HPLC yöntemi kullanılarak tespit edildi. Ekstraktlarda on beş fenolik bileşik tanımlandı. Kafeik asit, her iki ekstraktta da ana bileşendi. M. charantia ekstraktı ve D. stramonium ekstraktında kafeik asit miktarları sırasıyla 6282.51 ve 15183.36 µg g-1 olarak tespit edildi. D. stramonium yaprak ekstraktı, M. charantia meyve ekstraktından daha yüksek antioksidan aktivite gösterdi ve bu aktivite yüksek fenolik bileşik konsantrasyonları ile bağlantılı olabilir. Bu nedenle, bu bitkilerin çeşitli kısımlarından biyoaktif bileşiklerin taranması ile ilgili daha ileri çalışmalar, yeni doğal antioksidan kaynaklarının elde edilmesi için büyük önem taşıyacaktır.

Anahtar Kelimeler

Antioxidant Activity and Phenolic Composition of Ethanol Extracts of Momordica charantia and Datura stramonium

Abstract

Medicinal plants in the world are natural antioxidant sources as they contain some secondary metabolites such as phenolic acids and flavonoids. Therefore, they are used to prevent or treat many diseases in many parts of the world. However, the use of antioxidants in cancer treatment is still controversial. Accordingly, the phenolic composition and antioxidant potential of the ethanol extracts of Momordica charantia L. (Cucurbitaceae) and Datura stramonium L. (Solanaceae), which are used traditionally in Turkey and have cytotoxic potential on human cancer cells, were investigated. The antioxidant assays (DPPH, metal chelating, phosphomolybdenum, and ferric reducing power) were applied to the ethanol extracts of the fruits of M. charantia and the leaves of D. stramonium. The total phenolic and flavonoid contents were determined. The phenolic compounds contained in ethanolic extracts were identified using HPLC method. Fifteen phenolic compounds were identified in the extracts. Caffeic acid was the major constituent in both extracts. The amount of caffeic acid was detected to be 6282.51 and 15183.36 µg g-1 extract in M. charantia extract and the D. stramonium extracts, respectively. D. stramonium leaf extract showed higher antioxidant activity than M. charantia fruit extract and this activity may be associated with high phenolic compound concentrations. Hence, further studies about screening of bioactive compounds from various part of these plants will be a great importance for obtaining of source of novel natural antioxidants.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Mehlika Alper ^*
0000-0001-6193-346X
Türkiye

Cennet Özay
0000-0002-1120-6122
Türkiye

Yayımlanma Tarihi

28 Şubat 2022

Gönderilme Tarihi

31 Aralık 2020

Kabul Tarihi

9 Nisan 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.851251

IZ

https://izlik.org/JA77YR35TZ

Kaynak Göster

RIS / Bibtex

APA

Alper, M., & Özay, C. (2022). Antioxidant Activity and Phenolic Composition of Ethanol Extracts of Momordica charantia and Datura stramonium. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(1), 1-9. https://doi.org/10.18016/ksutarimdoga.vi.851251