İstiridye Mantarının (Pleurotus ostreatus) Farklı Flaş Zamanlarına Göre Antioksidan Aktivitesinin ve Hiperglisemide Anahtar Enzim Rolü Olan α -amilaz ve α -glukozidaz İnhibisyon Potansiyelinin Belirlenmesi

Nurcan Doğan; Cemhan Doğan

doi:10.18016/ksutarimdoga.vi.853006

EN TR

Determination of Antioxidant Activity of Oyster Mushroom According to Different Flush Times and the Inhibition Potential of α -amylase and α-glucosidase, which are Key Enzyme Role in Hyperglycemia

Abstract

Pleurotus ostreatus is a macro-mushroom known worldwide for its taste, high nutritional value, and medicinal properties, and was one of the first cultivated mushroom. However, a major problem is that the flush time is not paid attention to causing some unwanted changes in sensory, nutritional, and bioactive properties. In this study, the extraction conditions of mushroom powders of mature fruit (OM) and over-ripe fruit (AOM) were optimized by Response Surface Method (RSM). Besides, the total phenolic content (TPC), antioxidant activity (DPPH, FRAP, and ABTS), and the inhibition effect of α-amylase and α-glucosidase, which are key enzymes in hyperglycemia, have been demonstrated for mushroom powders extracted under optimum conditions. TPC was lost 6.93%, DPPH, FRAP and ABTS values 19.48%, 24.87%, and 16.91% respectively in over-ripe fruits. In addition, α-glucosidase inhibition potential decreased by 7.33% with delay of harvest time. In order to exhibit higher bioactive properties of Pleurotus ostreatus, flush time should be considered.

Keywords

İstiridye Mantarının (Pleurotus ostreatus) Farklı Flaş Zamanlarına Göre Antioksidan Aktivitesinin ve Hiperglisemide Anahtar Enzim Rolü Olan α -amilaz ve α -glukozidaz İnhibisyon Potansiyelinin Belirlenmesi

Öz

Pleurotus ostreatus, lezzeti, yüksek besleyici değeri ve tıbbi özellikleri ile dünya çapında bilinen ve ilk kültüre alınan mantarlardandır. Ancak flaş zamanına dikkat edilmemesi ve buna bağlı olarak duyusal, besinsel ve biyoaktif özelliklerinde birtakım istenmeyen değişmeler meydana gelmesi önemli bir problemdir. Bu çalışmada olgun meyve (OM) ve aşırı olgun meyvelere (AOM) ait mantar tozlarının ekstraksiyon şartları Yanıt Yüzey Yöntemi (YYY) ile optimize edilmiştir. Ayrıca optimum şartlarda ekstraksiyonu yapılan mantar tozlarının, toplam fenolik madde miktarı (TFMM), antioksidan aktivitesi (DPPH, FRAP ve ABTS) ve hiperglisemide anahtar enzim rolü olan α-amilaz ve α-glukozidaz inhibisyon etkisi ortaya konulmuştur. AOM’lerde TFMM %6.93, DPPH, FRAP ve ABTS değerleri ise sırasıyla %19.48, %24.87 ve %16.91 oranında kayba uğramıştır. Ayrıca α-glukozidaz inhibisyon potansiyeli hasat zamanının geciktirilmesi ile %7.33 oranında azalmıştır. Pleurotus ostreatus’un daha yüksek biyoaktif faydalar sergilemesi açısından flaş zamanı, dikkate alınması gereken önemli konular arasında olmalıdır.

Anahtar Kelimeler

Supporting Institution

Yozgat Bozok Üniversitesi Proje Koordinasyon Uygulama ve Araştırma Merkezi

Project Number

6602b-BMYO/17-125

References

Benzie I, Strain J 1996. The ferric reducing ability of plasma (FRAP) as a measure of " antioxidant power": the FRAP assay. Analytical biochemistry15: 1.
Brand-Williams B, Cuvelier M, Berset C 1995. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 28: 25-30.
Cam M, Basyigit B, Alasalvar H, Yilmaztekin M, Ahhmed A, Sagdic O, Konca Y, Telci I 2020. Bioactive properties of powdered peppermint and spearmint extracts: Inhibition of key enzymes linked to hypertension and type 2 diabetes. Food Bioscience: 100577.
Carabias-Martínez R, Rodríguez-Gonzalo E, Revilla-Ruiz P, Hernández-Méndez J 2005. Pressurized liquid extraction in the analysis of food and biological samples. Journal of Chromatography A 1089: 1-17.
Chen J, Li J-M, Tang Y-J, Ma K, Li B, Zeng X, Liu X-B, Li Y, Yang Z-L, Xu W-N 2020. Genome-wide analysis and prediction of genes involved in the biosynthesis of polysaccharides and bioactive secondary metabolites in high-temperature-tolerant wild Flammulina filiformis. BMC genomics 21: 1-16.
Cheung L, Cheung PC, Ooi VE 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food chemistry 81: 249-55.
Choi HY, Ryu H-S 2015. Antioxidant and anticancer effects of water extract from Pleurotus ostreatus. The Korean Journal of Food and Nutrition 28: 60-65.
Çam M, Hışıl Y, Durmaz G 2009. Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food chemistry 112: 721-26.

De Silva DD, Rapior S, Hyde KD, Bahkali AH 2012. Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Diversity 56: 1-29.
Dent M, Dragović-Uzelac V, Penić M, Bosiljkov T, Levaj B 2013. The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian wild sage (Salvia officinalis L.) extracts. Food technology and biotechnology 51: 84-91.
Doğan N, Doğan C, Çam M, Hayoğlu İ 2020. Optimization and comparison of three cooking methods for wheat flour‐oyster mushroom (P. ostreatus) powder composite chips. Journal of Food Processing and Preservation: e14873.
Friedman J, Hastie T, Tibshirani R 2001. The elements of statistical learning (Springer series in statistics New York).
Friedman M 2016. Mushroom polysaccharides: chemistry and antiobesity, antidiabetes, anticancer, and antibiotic properties in cells, rodents, and humans. Foods 5: 80.
Jayakumar T, Ramesh E, Geraldine P 2006. Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats. Food and Chemical Toxicology 44: 1989-96.
Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. The American journal of medicine 113: 71-88.
Lebovitz HE 1995. A new oral therapy for diabetes management: alpha-glucosidase inhibition with acarbose. Clinical diabetes 13: 99-104.
Li Q-Z, Wu D, Chen X, Zhou S, Liu Y, Yang Y, Cui F 2015. Chemical compositions and macrophage activation of polysaccharides from Leon's mane culinary-medicinal mushroom Hericium erinaceus (higher basidiomycetes) in different maturation stages. International Journal of Medicinal Mushrooms 17.
Lin M-Y, Yen C-L 1999. Antioxidative ability of lactic acid bacteria. Journal of agricultural and food chemistry 47: 1460-66.
Liu J, Vijayakumar C, Hall III CA, Hadley M, Wolf‐Hall CE 2005. Sensory and chemical analyses of oyster mushrooms (Pleurotus sajor‐caju) harvested from different substrates. Journal of food science 70: S586-S92.
Liyana-Pathirana C, Shahidi F 2005. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food chemistry 93: 47-56.
Markom M, Hasan M, Daud WRW, Singh H, Jahim JM 2007. Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods. Separation and purification technology 52: 487-96.
Mata G, Torres-Hernández F 2008. Effect of aerobic fermentation substrate on Pleurotus ostreatus production and resistance to Trichoderma viride. In Mushroom biology and mushroom products. Proceedings of the Sixth International Conference on Mushroom Biology and Mushroom Products, Bonn, Germany, 29 September-3 October, 2008, 74-82. GAMU GmbH, Institut für Pilzforschung.
Mau J-L, Chyau C-C, Li J-Y, Tseng Y-H 1997. Flavor compounds in straw mushrooms Volvariella volvacea harvested at different stages of maturity. Journal of agricultural and food chemistry 45: 4726-29.
McDougall GJ, Shpiro F, Dobson P, Smith P, Blake A, Stewart D 2005. Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase. Journal of agricultural and food chemistry 53: 2760-66.
Mourão F, Umeo SH, Takemura OS, Linde GA, Colauto NB 2011. Antioxidant activity of Agaricus brasiliensis basidiocarps on different maturation phases. Brazilian Journal of Microbiology 42: 197-202.
Muraguchi H, Umezawa K, Niikura M, Yoshida M, Kozaki T, Ishii K, Sakai K, Shimizu M, Nakahori K, Sakamoto Y 2015. Strand-specific RNA-seq analyses of fruiting body development in Coprinopsis cinerea. PLoS One 10: e0141586.
Myers RH, Montgomery DC, Anderson-Cook CM 1995. Response Surface Methodology: Process and Product Optimization Using Designed Experiments, John Wiley& Sons. Inc., New York, NY: 134-74.
Patel Y, Naraian R, Singh V 2012. Medicinal properties of Pleurotus species (oyster mushroom): a review. World Journal of Fungal and Plant Biology 3: 1-12.
Patil SS, Ahmed SA, Telang SM, Baig MMV 2010. The nutritional value of Pleurotus ostreatus (Jacq.: Fr.) kumm cultivated on different lignocellulosic agrowastes. Innovative Romanian food biotechnology.
Ravi B, Renitta RE, Prabha ML, Issac R, Naidu S 2013. Evaluation of antidiabetic potential of oyster mushroom (Pleurotus ostreatus) in alloxan-induced diabetic mice. Immunopharmacology and immunotoxicology 35: 101-09.
Saha AK, Acharya S, Roy A 2012. Antioxidant level of wild edible mushroom: Pleurotus djamor (Fr.) Boedijn. Journal of Agricultural Technology 8: 1343-51.
Sarangi I, Ghosh D, Bhutia SK, Mallick SK, Maiti TK 2006. Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans. International immunopharmacology 6: 1287-97.
Sarıkürkçü C, Karslı Semiz D, Solak M, Harmandar M 2004. Muğla Yöresi Yenilebilir Mantar Ekstraktlarının Antioksidant Aktivitelerinin Belirlenmesi. Türkiye 8: 26-28.
Singh R, Mishra K 2008. Mushroom cultivation. Ind.
Singleton VL, Orthofer R, Lamuela-Raventós RM 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology 299: 152-78.
Snyder L 1974. Classification of the solvent properties of common liquids. Journal of Chromatography A 92: 223-30.
Soares AA, de Souza CGM, Daniel FM, Ferrari GP, da Costa SMG, Peralta RM 2009. Antioxidant activity and total phenolic content of Agaricus brasiliensis (Agaricus blazei Murril) in two stages of maturity. Food chemistry 112: 775-81.
Song H-Y, Kim D-H, Kim J-M 2018. Comparative transcriptome analysis of dikaryotic mycelia and mature fruiting bodies in the edible mushroom Lentinula edodes. Scientific reports 8: 1-15.
Stamets P 2011. Growing gourmet and medicinal mushrooms (Ten speed press).
Stojkovic D, Smiljkovic M, Ciric A, Glamoclija J, Van Griensven L, Ferreira IC, Sokovic M 2019. An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes. South African Journal of Botany 120: 100-03.
Su C-H, Lai M-N, Ng L-T 2013. Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase–enzymes related to hyperglycemia. Food & function 4: 644-49.
Velioglu Y, Mazza G, Gao L, Oomah B 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of agricultural and food chemistry 46: 4113-17.
Wang Y, Zeng X, Liu W 2018. De novo transcriptomic analysis during Lentinula edodes fruiting body growth. Gene 641: 326-34.
Wasser S 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Applied microbiology and biotechnology 60: 258-74.
Zeng B, Chen K, Du P, Wang SS, Ren B, Ren YL, Yan HS, Liang Y, Wu FH 2016. Phenolic Compounds from Clinopodium chinense (Benth.) O. Kuntze and Their Inhibitory Effects on α‐Glucosidase and Vascular Endothelial Cells Injury. Chemistry & Biodiversity 13: 596-601.
Zhang Y, Hu T, Zhou H, Zhang Y, Jin G, Yang Y 2016. Antidiabetic effect of polysaccharides from Pleurotus ostreatus in streptozotocin-induced diabetic rats. International journal of biological macromolecules 83: 126-32.

Details

Primary Language

Turkish

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Nurcan Doğan ^*
0000-0001-5414-1819
Türkiye

Cemhan Doğan
0000-0002-9043-0949
Türkiye

Publication Date

December 31, 2021

Submission Date

January 3, 2021

Acceptance Date

March 18, 2021

Published in Issue

Year 2021 Volume: 24 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.853006

IZ

https://izlik.org/JA43LK92MC

Cite

RIS / Bibtex

APA

Doğan, N., & Doğan, C. (2021). İstiridye Mantarının (Pleurotus ostreatus) Farklı Flaş Zamanlarına Göre Antioksidan Aktivitesinin ve Hiperglisemide Anahtar Enzim Rolü Olan α -amilaz ve α -glukozidaz İnhibisyon Potansiyelinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(6), 1177-1186. https://doi.org/10.18016/ksutarimdoga.vi.853006

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