İstiridye Mantarının (Pleurotus ostreatus) Farklı Flaş Zamanlarına Göre Antioksidan Aktivitesinin ve Hiperglisemide Anahtar Enzim Rolü Olan α -amilaz ve α -glukozidaz İnhibisyon Potansiyelinin Belirlenmesi

Nurcan Doğan; Cemhan Doğan

doi:10.18016/ksutarimdoga.vi.853006

EN TR

Determination of Antioxidant Activity of Oyster Mushroom According to Different Flush Times and the Inhibition Potential of α -amylase and α-glucosidase, which are Key Enzyme Role in Hyperglycemia

Abstract

Pleurotus ostreatus is a macro-mushroom known worldwide for its taste, high nutritional value, and medicinal properties, and was one of the first cultivated mushroom. However, a major problem is that the flush time is not paid attention to causing some unwanted changes in sensory, nutritional, and bioactive properties. In this study, the extraction conditions of mushroom powders of mature fruit (OM) and over-ripe fruit (AOM) were optimized by Response Surface Method (RSM). Besides, the total phenolic content (TPC), antioxidant activity (DPPH, FRAP, and ABTS), and the inhibition effect of α-amylase and α-glucosidase, which are key enzymes in hyperglycemia, have been demonstrated for mushroom powders extracted under optimum conditions. TPC was lost 6.93%, DPPH, FRAP and ABTS values 19.48%, 24.87%, and 16.91% respectively in over-ripe fruits. In addition, α-glucosidase inhibition potential decreased by 7.33% with delay of harvest time. In order to exhibit higher bioactive properties of Pleurotus ostreatus, flush time should be considered.

Keywords

İstiridye Mantarının (Pleurotus ostreatus) Farklı Flaş Zamanlarına Göre Antioksidan Aktivitesinin ve Hiperglisemide Anahtar Enzim Rolü Olan α -amilaz ve α -glukozidaz İnhibisyon Potansiyelinin Belirlenmesi

Öz

Pleurotus ostreatus, lezzeti, yüksek besleyici değeri ve tıbbi özellikleri ile dünya çapında bilinen ve ilk kültüre alınan mantarlardandır. Ancak flaş zamanına dikkat edilmemesi ve buna bağlı olarak duyusal, besinsel ve biyoaktif özelliklerinde birtakım istenmeyen değişmeler meydana gelmesi önemli bir problemdir. Bu çalışmada olgun meyve (OM) ve aşırı olgun meyvelere (AOM) ait mantar tozlarının ekstraksiyon şartları Yanıt Yüzey Yöntemi (YYY) ile optimize edilmiştir. Ayrıca optimum şartlarda ekstraksiyonu yapılan mantar tozlarının, toplam fenolik madde miktarı (TFMM), antioksidan aktivitesi (DPPH, FRAP ve ABTS) ve hiperglisemide anahtar enzim rolü olan α-amilaz ve α-glukozidaz inhibisyon etkisi ortaya konulmuştur. AOM’lerde TFMM %6.93, DPPH, FRAP ve ABTS değerleri ise sırasıyla %19.48, %24.87 ve %16.91 oranında kayba uğramıştır. Ayrıca α-glukozidaz inhibisyon potansiyeli hasat zamanının geciktirilmesi ile %7.33 oranında azalmıştır. Pleurotus ostreatus’un daha yüksek biyoaktif faydalar sergilemesi açısından flaş zamanı, dikkate alınması gereken önemli konular arasında olmalıdır.

Anahtar Kelimeler

Destekleyen Kurum

Yozgat Bozok Üniversitesi Proje Koordinasyon Uygulama ve Araştırma Merkezi

Proje Numarası

6602b-BMYO/17-125

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Nurcan Doğan ^*
0000-0001-5414-1819
Türkiye

Cemhan Doğan
0000-0002-9043-0949
Türkiye

Yayımlanma Tarihi

31 Aralık 2021

Gönderilme Tarihi

3 Ocak 2021

Kabul Tarihi

18 Mart 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.853006

IZ

https://izlik.org/JA43LK92MC

Kaynak Göster

RIS / Bibtex

APA

Doğan, N., & Doğan, C. (2021). İstiridye Mantarının (Pleurotus ostreatus) Farklı Flaş Zamanlarına Göre Antioksidan Aktivitesinin ve Hiperglisemide Anahtar Enzim Rolü Olan α -amilaz ve α -glukozidaz İnhibisyon Potansiyelinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(6), 1177-1186. https://doi.org/10.18016/ksutarimdoga.vi.853006