Cytogenetic Analysis of Alburnus escherichii (Teleostei: Leuciscidae) in Turkey

Zafer Alpaslan; Atilla Arslan

doi:10.18016/ksutarimdoga.vi.876754

EN TR

Cytogenetic Analysis of Alburnus escherichii (Teleostei: Leuciscidae) in Turkey

Abstract

In this study, cytogenetic properties of A. escherichii were investigated using standard Giemsa staining, C-banding, and Ag-NOR staining techniques. The karyotype of Sakarya bleak, the diploid chromosome number was 2n = 50, consists of six pairs of metacentric, 12 pairs of submetacentric, and seven pairs of acrocentric autosomes (NF=86). No morphologically distinguishable heteromorphic sex chromosomes were detected in the karyotype. While most autosomes had centromeric and pericentromeric C-heterochromatin (some chromosomes slightly) blocks, the other autosomal pairs were C-negative. Also, the short arm of the eleventh submetacentric chromosome pair was entirely C-positive. The nucleolar organizer regions were localized in medium-sized submetacentric autosomal pairs. Active NORs associated with the C-heterochromatin were observed in the whole of the short arm of the eleventh chromosome pair, and their active NORs were homomorphic. The banded karyotypes of A. escherichii were reported in this study for the first time. Significant findings obtained in this research may contribute to cytotaxonomy of Alburnus species in Anatolian and Europe.

Keywords

Türkiye’deki Alburnus escherichii’nin (Teleostei: Leuciscidae) Sitogenetik Analizi

Öz

Bu çalışmada A. escherichii'nin sitogenetik özellikleri, standart Giemsa boyama, C-bantlama ve Ag-NOR boyama teknikleri kullanılarak araştırılmıştır. Diploid kromozom sayısı 2n = 50 olan Sakarya İnci balığının karyotipi altı çift metasentrik, 12 çift submetasentrik ve yedi çift akrosentrik otozomdan (NF = 86) oluşmaktadır. Karyotipte morfolojik olarak ayırt edilebilir heteromorfik cinsiyet kromozomu tespit edilmedi. Çoğu otozom, sentromerik ve perisentromerik C-heterokromatin (bazı kromozomlar hafif) bloklarına sahipken, diğer otozomal çiftler C-negatiftir. Ayrıca, on birinci submetasentrik kromozom çiftinin kısa kolu tamamen C-pozitifti. Nükleolar düzenleyici bölgeler, orta büyüklükte submetasentrik otozomal çiftler halinde lokalize idi. C-heterokromatin ile ilişkili aktif NOR'lar, on birinci kromozom çiftinin kısa kolunun tamamında gözlendi ve aktif NOR'lar homomorfikti. A. escherichii'nin bantlı karyotipleri ilk kez bu çalışmada rapor edilmiştir. Bu araştırmada elde edilen önemli bulgular, Anadolu ve Avrupa'daki Alburnus türlerinin sitotaksonomisine katkı sağlayabilir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Zafer Alpaslan
0000-0003-4555-0447
Türkiye

Atilla Arslan ^*
0000-0002-4766-4969
Türkiye

Publication Date

April 30, 2022

Submission Date

February 8, 2021

Acceptance Date

May 27, 2021

Published in Issue

Year 2022 Volume: 25 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.876754

IZ

https://izlik.org/JA56JW53LZ

Cite

RIS / Bibtex

APA

Alpaslan, Z., & Arslan, A. (2022). Cytogenetic Analysis of Alburnus escherichii (Teleostei: Leuciscidae) in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(2), 297-304. https://doi.org/10.18016/ksutarimdoga.vi.876754