Fumaria parviflora Lam. ve Fumaria capreolata L.’nin Antioksidan ve Sitotoksik Aktiviteleri

Tülay Aşkın Çelik; Özlem Sultan Aslantürk; Ebru Şebnem Yılmaz; Yelda Güzel

doi:10.18016/ksutarimdoga.vi.899937

TR EN

Fumaria parviflora Lam. ve Fumaria capreolata L.’nin Antioksidan ve Sitotoksik Aktiviteleri

Öz

Bu çalışmada, Fumaria parviflora ve Fumaria capreolata metanol özütlerinin in vitro antioksidan aktiviteleri ile iki farklı insan hepatosellüler karsinoma (Hep3B ve HepG2) hücre hattı üzerindeki sitotoksik etkileri araştırılmıştır. Özütlerin antioksidan aktiviteleri 1,1-diphenyl-2-picrylhydrazyl (DPPH) radikalini süpürme ve fosfomolibdat testi ile belirlenmiştir. Hücre canlılığı ise 24, 48 ve 72 saatlik farklı özüt konsantrasyonları (25-250 µgmL-1) ile muamele sonrasında MTT testi ile belirlenmiştir. F. parviflora ve F. capreolata metanol özütleri serbest DPPH süpürme aktivitesi ve toplam antioksidan kapasitesi açısından karşılaştırıldığında, F. parviflora metanol özütü hem DPPH serbest radikalini süpürme hem de toplam antioksidan kapasitesi bakımından yüksek aktiviteye sahiptir. MTT assay sonuçları, F. capreolata metanol özütünün 24 ve 48 saatlik muameleden sonra Hep3B hücrelerinde hücre çoğalmasını önemli ölçüde artırdığını ortaya çıkarmıştır. 72 saatlik uygulama sonrası, metanol özütü Hep3B hücrelerinin çoğalmasını zamana ve konsantrasyona bağlı şekilde hafif inhibe etmiştir. F. parviflora özütü, Hep3B hücreleri üzerinde sadece 72 saatlik uygulamada yüksek sitotoksik etkiye sahipken, F. capreolata bu hücreler üzerinde önemli bir sitotoksik etki göstermemiştir.

Anahtar Kelimeler

Antioxidant and Cytotoxic Activities of Fumaria parviflora Lam. and Fumaria capreolata L.

Abstract

This study was carried out to examine the in vitro antioxidant and cytotoxic effects of methanol extracts of Fumaria parviflora Lam. and Fumaria capreolata. Antioxidant assays carried out by employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and phosphomolybdate assays. Cell viability was quantified by employing MTT assay for 24, 48 and 72 h of exposures to 25-250 µgmL-1 concentrations of the extracts. F. parviflora methanol extract possessed the highest antioxidant activities in both free DPPH scavenging and total antioxidant capacity assays. Results from MTT assay revealed that F. capreolata methanol extract significantly increased the cell proliferation in human hepatocellular carcinoma (Hep3B) cells after 24 and 48 hours of treatment. After 72 hours of treatment, methanol extract slightly inhibited the proliferation of Hep3B cell lines, in a time-and dose-dependent manner. While F. parviflora extract had the highest cytotoxic effect on Hep3B cells after 72 hours of the treatment. F. capreolata did not cause any significant cytotoxic effect on these cell lines.

Keywords

Supporting Institution

HMKU Scientific Research Project Fund .

Project Number

18.YL.010

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Tülay Aşkın Çelik ^*
0000-0001-6891-9089
Türkiye

Özlem Sultan Aslantürk
0000-0002-2503-0164
Türkiye

Ebru Şebnem Yılmaz
0000-0001-6124-4832
Türkiye

Yelda Güzel
0000-0002-7975-3130
Türkiye

Publication Date

August 31, 2022

Submission Date

March 19, 2021

Acceptance Date

July 16, 2021

Published in Issue

Year 2022 Volume: 25 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.899937

IZ

https://izlik.org/JA56WF69GH

Cite

RIS / Bibtex

APA

Aşkın Çelik, T., Aslantürk, Ö. S., Yılmaz, E. Ş., & Güzel, Y. (2022). Fumaria parviflora Lam. ve Fumaria capreolata L.’nin Antioksidan ve Sitotoksik Aktiviteleri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(4), 819-827. https://doi.org/10.18016/ksutarimdoga.vi.899937

Cited By

In Vitro Cytotoxic Effects of Some Fumaria L. (Papaveraceae) Species Methanolic Extracts on Cancer Cell Lines

Türk Doğa ve Fen Dergisi

https://doi.org/10.46810/tdfd.1287018