Optimization Extraction of Cladonia foliacea (Huds.) Willd: Antioxidant Activity and Inhibition of the Key Enzymes Linked to Type II Diabetes

Nurcan Doğan; Cemhan Doğan; Mustafa Kocakaya

doi:10.18016/ksutarimdoga.vi.908382

EN TR

Optimization Extraction of Cladonia foliacea (Huds.) Willd: Antioxidant Activity and Inhibition of the Key Enzymes Linked to Type II Diabetes

Abstract

Cladonia foliacea (CF) is a type of lichen belonging to the Cladoniaceae family, used as traditional medicine for various diseases. It is known that CF has strong antioxidant and antidiabetic effects originating from various secondary components. However, the bioactivity of CF is significantly affected by extraction conditions such as temperature, liquid/solid ratio, and solvent type/concentration. Herein, the extraction parameters (temperature, liquid/solid ratio, and methanol concentration) of CF were optimized by response surface methodology (RSM) based on maximum total phenolic content (TPC), antioxidant capacity, and α-glucosidase/α-amylase inhibitor activity. In the methanolic extraction of CF, 48.8 °C, 12.3 mL g-1 liquid/solid ratio, and 86.4% methanol concentration were determined as the optimum point. TPC, antioxidant capacity, α-glucosidase, and α-amylase inhibition activities of CF extracted under optimum conditions were determined as 5.55 mg GAE g-1, 33.10 g sample/g DPPH, 68.78%, and 50.03%, respectively. These results suggest that extraction conditions may be a limiting factor in terms of bioactive properties and optimized extraction parameters may improve the potential antioxidant and inhibitory activity of key enzymes associated with type II diabetes of CF.

Keywords

Tip II Diyabet ve Oksidasyon ile İlişkili Enzimlerin ve Radikallerin İnhibisyonunun Hedefinde Cladonia foliacea (Huds.) Willd'in Ekstraksiyonun Optimizasyonu

Öz

Cladonia foliacea (CF), çeşitli hastalıklar için geleneksel ilaç olarak kullanılan Cladoniaceae familyasına ait bir liken türüdür. CF’nin çeşitli sekonder bileşiklerden kaynaklanan güçlü antioksidan ve antidiyabetik etkilere sahip olduğu bilinmektedir. Ancak CF’nin biyoaktivitesi sıcaklık, sıvı/katı oranı ve solvent çeşidi/konsantrasyonu gibi ekstraksiyon şartlarından önemli ölçüde etkilenmektedir. Bu nedenle, bu çalışmada CF’nin ekstraksiyon parametreleri (sıcaklık, sıvı/katı oranı ve metanol konsantrasyonu) maksimum toplam fenolik madde miktarı (TFMM), antioksidan kapasitesi ve α-glukozidaz/α-amilaz inhibisyon aktivitesi baz alınarak yanıt yüzey yöntemi (YYY) ile optimize edilmiştir. CF'nin metanolik ekstraksiyonunda 48.8 °C, 12.3 mL g-1 sıvı/katı oranı ve %86.4 metanol konsantrasyonu optimum nokta olarak belirlenmiştir. Optimum koşullar altında ekstrakte edilen CF'nin TPC, antioksidan kapasitesi, α-glukosidaz ve α-amilaz inhibisyon aktiviteleri sırasıyla 5.55 mg GAE g-1, 33.10 g örnek/g DPPH, %68.78 ve %50.03 olarak belirlenmiştir. Bu sonuçlar, ekstraksiyon koşullarının biyoaktif özellikler açısından sınırlayıcı bir faktör olabileceğini ve optimize edilmiş ekstraksiyon parametrelerinin CF'nin potansiyel antioksidan ve tip II diyabetle bağlantılı anahtar enzimlerin inhibisyon aktivite etkisinin iyileştirebileceğini düşündürmektedir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Nurcan Doğan ^*
0000-0001-5414-1819
Türkiye

Cemhan Doğan
0000-0002-9043-0949
Türkiye

Mustafa Kocakaya
0000-0003-2306-8094
Türkiye

Publication Date

August 31, 2022

Submission Date

April 2, 2021

Acceptance Date

July 29, 2021

Published in Issue

Year 2022 Volume: 25 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.908382

IZ

https://izlik.org/JA33LR77HF

Cite

RIS / Bibtex

APA

Doğan, N., Doğan, C., & Kocakaya, M. (2022). Optimization Extraction of Cladonia foliacea (Huds.) Willd: Antioxidant Activity and Inhibition of the Key Enzymes Linked to Type II Diabetes. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(4), 828-837. https://doi.org/10.18016/ksutarimdoga.vi.908382

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