Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.

Çiğdem Alev Özel; Siti Maesaroh

doi:10.18016/ksudobil.386167

TR EN

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Öz

Indigofera zollingeriana, Endonezya'da yaygın olarak yetişen proteince zengin yem ve boya bitkisidir. Bitkiyi tohumdan çoğaltmak yüksek dormansi sebebiyle zor olmaktadır. Bu nedenle, I. zollingeriana'nın in vitro ve ex vitro koşulllarda çoğaltma tekniklerinin geliştirilmesi gerekmektedir. Bu çalışmada, farklı kombinasyonlarda BAP +0.01 mg/L NAA içeren MS ortamı kullanılarak in vitro koşullar altında bitkinin hızlı çoğaltım için bir protokol geliştirmesi amaçlanmıştır. En fazla sürgün rejenerasyonu epigeal ve kotiledon nodlarının sırasıyla 1.5 mg / L BAP + 0.01 mg / L NAA ve 1.0 mg/L BAP + 0.01 mg/L NAA içeren MS ortamında elde edilmiştir. BAP + NAA'nın tüm kombinasyonlarında rejenerasyonu yapılan sürgünler 0.5 mg/L IBA kullanılarak başarılı şekilde köklendirilmiştir. Köklü bitkiler, bir iklim dolabında dış koşullara alıştırılmıştır. Bu çalışma I. zollingeriana bitkisinin in vitro koşullar altında yetiştirilebileceğini göstermiş olması ve elde edilen sonuçların ilerideki çalışmalarda aktif bileşiklerin biyosentezi ve ekstraksiyonu ile bitki yetiştirilmesi ve çoğaltılmasına yardımcı olacağını düşünülmektedir.

Anahtar Kelimeler

oksin,kotiledon nod,sitokinin epigeal nod,in vitro

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Çiğdem Alev Özel ^*

Siti Maesaroh

Publication Date

October 31, 2018

Submission Date

January 30, 2018

Acceptance Date

May 25, 2018

Published in Issue

Year 1970 Volume: 21 Number: 5

DOI

https://doi.org/10.18016/ksudobil.386167

IZ

https://izlik.org/JA97GL37PP

Cite

RIS / Bibtex

APA

Özel, Ç. A., & Maesaroh, S. (2018). Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(5), 672-677. https://doi.org/10.18016/ksudobil.386167

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Multiple shoot regeneration of an important equatorial forage plant Indigofera zollingeriana Miq.

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Öz

Anahtar Kelimeler