Year 2019, Volume 22 , Issue 3, Pages 360 - 369 2019-06-30

Ayçiçeği Bitkisi Yapraklarında Tuz Stresi, Nitrik Oksit ve Hormon Uygulamalarının Antioksidan Savunma Sistemi Üzerine Etkileri
The Effects of Salt Stress, Nitric Oxide and Hormone Applications on Antioxidant Defense in Sunflower Plant Leaves

Oğuz Ayhan KİREÇCİ [1] , Füsun YÜREKLİ [2]


Bu çalışmada ayçiçeği (Helianthus annuus L. cv. Tarsan-1018 çeşidi) bitkisinde tuz stresi, sodyum nitroprussid ve bitki hormonları uygulamalarının antioksidan sistem aktiviteleri üzerine etkilerinin araştırılması amaçlanmıştır. Çalışma 2016 yılında kontrollü iklim odası şartlarında yapılmıştır.  Tohumlar 5 hafta süreyle kültür çözeltisi ile sulanarak, 5. hafta sonunda tuz, sodyum nitroprussid ve bitki hormonları uygulamaları 48 saat süreyle yapılmıştır.  48. Saat sonunda örnekler alınarak,   süperoksid dismutaz aktivitesi Sairam ve ark. (2002)’ a   katalaz aktivitesi Aebi (1984)’e    glutatyon S transferaz aktivitesi Habig ve ark. (1974)’ e ve prolin miktarı da Ninhiydrin (Troll ve Lindsley, 1955) metoduna göre belirlenmiştir.  Sonuçlar; tuz stresi ve Sodyum nitroprussid uygulamalarının antioksidan savunmayı teşvik ettiğini göstermıitır.  Bitki hormonları farklı etkiler yapmış olup; absisik asidin antioksidan savunma sistemi üzerine olumlu etkilerde bulunduğu, gibberellik asidin ise enzim aktiviteleri ve prolin miktarı üzerine etkisinin olumsuz olduğu belirlenmiştir. 



In this study, effects of salt stress, SNP and plant hormones on acticities of antioxidant system in sunflower (Helianthus annuus L. cv. Tarsan-1018) leaf tissues were investigated. The study was conducted in 2016 under controlled climate room conditions. The seeds were irrigated with culture solution for 5 weeks. At the end of the fifth week, salt, SNP and hormone applications were applied for 48 hours. At the end of 48-hour samples were gathered. Superoxide dismutase activity, Catalase activity, Glutathione S transferase activity and  Proline amount were determined according to Sairam et al. (2002), Aebi (1984), Habig et al. (1974) and Ninhiydrin method (Troll and Lindsley, 1955), respectively. Results showed that salt stress and Sodium nitroprusside stimulated antioxidant defense. Plant hormones had different effects. Abscisic acid had positive effects on antioxidant defense. Gibberellic acid caused negative effects on enzyme activities and proline amount.

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Primary Language tr
Subjects Science
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0003-2205-4758
Author: Oğuz Ayhan KİREÇCİ (Primary Author)
Country: Turkey


Orcid: 0000-0002-4111-9230
Author: Füsun YÜREKLİ
Country: Turkey


Dates

Application Date : September 7, 2018
Acceptance Date : January 30, 2019
Publication Date : June 30, 2019

APA Ki̇reçci̇, O , Yürekli̇, F . (2019). Ayçiçeği Bitkisi Yapraklarında Tuz Stresi, Nitrik Oksit ve Hormon Uygulamalarının Antioksidan Savunma Sistemi Üzerine Etkileri . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 (3) , 360-369 . DOI: 10.18016/ksutarimdoga.vi.457992