Research Article
BibTex RIS Cite

Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi

Year 2019, Volume: 22 Issue: 3, 456 - 465, 30.06.2019

Sabahattin Cömertpay , Gizem Ceylan

https://doi.org/10.18016/ksutarimdoga.vi.496108
Cited By: 1

Abstract

Malignant
mezoteliyoma akciğer, kalp ve karın zarında gelişen bir kanser türüdür.
Kapsaisin, Capsicum spp.cinsi bitkilerde üretilen ikincil bir metabolittir ve
bazı kanser hücreleri üzerinde sitotoksik etkisi olduğu gösterilmiştir. Afidokolin
ve nokadozol, hücreleri sırasıyla G1/S ve G2/M fazında tutar. Bu çalışmada,
kanserli (H2452) ve ölümsüzleştirilmiş (AG07086) mezotelyum hücrelerinin
kapsaisin sitotoksisitesi, afidokolin ve nokodazol ön muamelesi ile, ya da bu
muameleler olmaksızın, MTS tekniğiyle belirlenmiş ve kaspaz-3 mRNA
seviyesindeki değişiklikler qRT-PCR metoduyla incelenmiştir. Elde edilen
bulgulara göre; i) normal
koşullarda, kanserli ve ölümsüzleştirilmiş mezotelyum hücrelerinde kapsaisin
sitotokisitesi benzerdir (p>0.05), ii)
afidikolin muamelesiyle kapsaisinin etkenliği azalmış (p<0.05) ancak kanser
hücrelerinin kapsaisin duyarlılığı ölümsüz hücrelere kıyasla yükselmiştir
(p<0.05), iii) nokodazol ön
muamelesi ile, kanser hücrelerinde kapsaisinin sitotoksik etkisi düşüş
göstermiş (p<0.05), ancak ölümsüzleştirilmiş hücreler için bu değişim
istatiksel olarak anlamsız bulunmuştur (p>0.05), iv) yapılan tüm uygulamalarda kaspaz-3 mRNA miktarı kontrol grubuna
denk çıkmıştır (p>0.05). 

Keywords

Mezotelyoma Kapsaisin Afidikolin Nokodazol Sitotoksisite

References

  • Arooz T, Yam CH, Siu WY, Lau A, Li KKW, Poon RYC 2000. On the Concentrations of Cyclins and Cyclin-Dependent Kinases in Extracts of Cultured Human Cells. Biochemistry, 39(31): 9494-9501.
  • Borel F, Lacroix FB, Margolis RL 2002. Prolonged Arrest of Mammalian Cells at the G1/S Boundary Results in Permanent S Phase Stasis. Journal of Cell Science 115: 2829-2838.
  • Cao S, Chen H, Xiang S, Hong J, Weng L, Zhu H, Liu Q 2015. Anti-Cancer Effects and Mechanisms of Capsaicin in Chili Peppers. American Journal of Plant Sciences, 06(19): 3075-3081.
  • Carbone M, Emri S, Dogan AU, Steele I, Tuncer M, Pass HI, Baris YI 2007. A Mesothelioma Epidemic in Cappadocia: Scientific Developments and Unexpected Social Outcomes. Nat Rev Cancer, 7(2): 147-154.
  • Carbone M, Kanodia S, Chao A, Miller A, Wali A, Weissman D, Adjei A, Baumann F, Boffetta P, Buck B, de Perrot M, Dogan AU, Gavett S, Gualtieri A, Hassan R, Hesdorffer M, Hirsch FR, Larson D, Mao W, Masten S, Pass HI, Peto J, Pira E, Steele I, Tsao A, Woodard GA, Yang H, Malik S 2016. Consensus Report of the 2015 Weinman International Conference on Mesothelioma. J Thorac Oncol, 11(8): 1246-1262.
  • Carbone M,Baris YI, Bertino P, Brass B, Comertpay S, Dogan A.U, Gaudino G, Jube S, Kanodia S, Partridge CR, Pass HI, Rivera ZS, Steele I, Tuncer M, Way S, Yang H, Aubrey M 2011. Erionite Exposure in North Dakota and Turkish Villages with Mesothelioma. PNAS, 108(33): 13618–13623.
  • Chow J, Norng M, Zhang J, Chai J 2007. TRPV6 Mediates Capsaicin-Induced Apoptosis in Gastric Cancer Cells—Mechanisms Behind A Possible New “Hot” Cancer Treatment. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1773(4): 565-576.
  • Clark R, Lee SH 2016. Anticancer Properties of Capsaicin Against Human Cancer. Anticancer Research, 36: 837-844.
  • Elmore S 2007. Apoptosis: A Review of Programmed Cell Death. Toxicol Pathol, 35(4): 495-516.
  • Emri SA 2017. The Cappadocia Mesothelioma Epidemic: Its Influence in Turkey and Abroad. Ann Transl Med, 5(11):239.
  • Emri S, Demir A, Dogan M, Akay H, Bozkurt B, Carbone M, Baris I 2002. Lung Diseases Due to Environmental Exposures to Erionite and Asbestos in Turkey. Toxicology Letters, 127(1–3): 251-257.
  • Haltiwanger RS, Philipsberg GA 1997. Mitotic Arrest with Nocodazole Induces Selective Changes in the Level of O-Linked N-Acetylglucosamine and Accumulation of Incompletely Processed N-Glycans on Proteins from HT29 Cells. The journal of biological chemistry, 272(13): 8752–8758.
  • Harashima H, Dissmeyer N, Schnittger A 2013. Cell Cycle Control Across the Eukaryotic Kingdom. Trends Cell Biol, 23(7): 345-356.
  • Helvacı N, Comertpay S 2018. in vitro Evaluation of The Effects of Capsaicin on Normal And Cancerous Cells of Human Cartilage. Turk J Biol, 42: 422-434.
  • Hiddinga BI, Rolfo C, van Meerbeeck JP 2015. Mesothelioma Treatment: Are We on Target? A review. J Adv Res, 6(3): 319-330.
  • Hochegger H, Takeda S, Hunt T 2008. Cyclin-Dependent Kinases and Cell-Cycle Transitions: Does One Fit All? Molecular Cell Biology, 9(11): 910-916.
  • Ho YS, Duh JS , Jeng JH, Wang YJ, Liang YC , Lin CH, Tseng CJ, Yu CF , Chen RJ, Lin JK 2001. Griseofulvin Potentiates Antitumorigenesis Effects of Nocodazole Through Induction of Apoptosis and G2/M Cell Cycle Arrest in Human Colorectal Cancer Cells. Int. J. Cancer 91: 393–401.
  • Holmes RE, Leddy LR 2014. Chondrosarcoma of Bone. Cancer Treat Res, 162: 117-130.
  • Huang SP, Chen JC, Wu CC, Chen CT, Tang NY, Ho YT, Lo C, Lin JP, Chung JG, Lin JG 2009. Capsaicin-induced Apoptosis in Human Hepatoma HepG2 Cells. Anticancer Research, 29: 165-174.
  • Igney FH, Krammer PH 2002. Death And Anti-Death: Tumour Resistance to Apoptosis. Nat Rev Cancer, 2(4): 277-288.
  • Jin T, Wu H, Wang Y, Peng H 2016. Capsaicin Induces Immunogenic Cell Death in Human Osteosarcoma Cells. Exp Ther Med, 12(2): 765-770.
  • Li H, Krstin S, Wang S, Wink M 2018. Capsaicin and Piperine Can Overcome Multidrug Resistance in Cancer Cells to Doxorubicin. Molecules, 23(3): 557.
  • Lowe SW, Lin AW 2000. Apoptosis in Cancer. Carcinogenesis, 21: 485–495.
  • Martinvalet D, Zhu P, Lieberman J 2005. Granzyme A Induces Caspase-Independent Mitochondrial Damage, A Required First Step For Apoptosis. Immunity, 22(3): 355-370.
  • Mori A, Lehmann S, O’Kelly J, Kumagai T, Desmond JC, Pervan M, McBride WH, Kizaki M, Koeffler H P 2006. Capsaicin, a Component of Red Peppers, Inhibits the Growth of Androgen-Independent, p53 Mutant Prostate Cancer Cells. Cancer Research, 66(6): 3222-3229
  • Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N 2013. Crosstalk Between Apoptosis, Necrosis and Autophagy. Biochim Biophys Acta, 1833(12): 3448-3459.
  • Poxleitner MK, Dawson SC, Cande WZ 2008. Cell Cycle Synchrony in Giardia intestinalis Cultures Achieved by Using Nocodazole and Aphidicolin. Eukaryot Cell, 7(4): 569-574.
  • Robinson BWS, Musk AW, Lake RA 2005. Malignant Mesothelioma. The Lancet, 366(9483): 397-408.
  • Roskoski JrR 2019. Cyclin-Dependent Protein Serine/Threonine Kinase Inhibitors As Anticancer Drugs. Pharmacological Research, 139:471-488.
  • Sanchez I, Dynlacht BD 2005. New Insights into Cyclins, Cdks, and Cell Cycle Control. Semin Cell Dev Biol, 16(3): 311-321.
  • Sargent JM, Elgie AW, Williamson CJ, Taylor CG 1996. Aphidicolin Markedly Increases the Platinum Sensitivity of Cells from Primary Ovarian Tumours. Britsh Journal of Cancer, 74: 1730-1733.
  • Starczewska E, Beyaert M, Michaux L, Vekemans MC, Saussoy P, Bol V, Echarri AA, Smal C, Neste EVD, Bontemps F 2016. Targeting DNA Repair with Aphidicolin Sensitizes Primary Chronic Lymphocytic Leukemia Cells to Purine Analogs. Oncotarget, 7(25):38367-38379.
  • Swaffer MP, Jones AW, Flynn HR, Snijders AP, Nurse P 2016. CDK Substrate Phosphorylation and Ordering the Cell Cycle. Cell, 167(7): 1750-1761.
  • Teixeira LK, Reed SI 2017. Cyclin E Deregulation and Genomic Instability. In: Masai H, Foiani M, Editors. DNA Replication: From Old Principles to New Discoveries. 1st ed. Singapore: Springer, pp. 527-547.
  • Truman AW, Kitazono AA, Fitz Gerald JN, Kron SJ 2012. Cell Cycle: Regulation by Cyclins. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net. (Erişim tarihi:11.12.18).
  • Vermeulen K, Bockstaele DRV, Berneman ZN 2003. The Cell Cycle: A Review of Regulation, Deregulation and Therapeutic Targets in Cancer. Cell Prolif., 36: 131–149.
  • Wilding JL, Bodmer WF 2014. Cancer Cell Lines For Drug Discovery and Development. Cancer Res, 74(9): 2377-2384.
  • Ye J, Zhang R, Wu F, Zhai L, Wang K, Xiao M, Xie T, Sui X 2018. Non-Apoptotic Cell Death in Malignant Tumor Cells and Natural Compounds. Cancer Lett, 420: 210-227.
  • Zhang R, Humphreys I, Sahu PR, Shi Y, Srivastava SK 2008. in vitro and in vivo Induction of Apoptosis by Capsaicin in Pancreatic Cancer Cells is Mediated Through ROS Generation and Mitochondrial Death Pathway. Apoptosis, 13(12):1465-1478.

Effect of Nocodazole and Aphidicolin Pre-treatment on Capsaicin Cytotoxicity in Malignant and Immortalized Cells of Mesothelium

Year 2019, Volume: 22 Issue: 3, 456 - 465, 30.06.2019

Sabahattin Cömertpay , Gizem Ceylan

https://doi.org/10.18016/ksutarimdoga.vi.496108
Cited By: 1

Abstract

Malignant mesothelioma is a type of cancer, developing from the lung membrane, heart and stomach. Capsaicin is a
secondary metabolite produced in Capsicum spp., which has been demonstrated to
have cytotoxic effect on various cancer types. Aphidicolin and nocadozole are
known to arrest cells in G1/S and G2/M, respectively. In this study, the
cytotoxicity of capsaicin on cancerous (H2452) and immortalized (AG07086) cells
of mesothelium, with or without primary exposure to aphidicolin or nocadozole,
was determined through MTS method and the fluctuations in caspase-3 mRNA levels
were investigated by qRT-PCR. Accordingly i)
under regular conditions, capsaicin cytotoxicity on cancerous and immortalized
cells of mesothelium was similar (p>0.05), ii) aphidicolin pre-exposure decreased the cytotoxicity of
capsaicin on cancerous cells (p<0.05), yet it increased their sensitivity to
capsaicin (p<0.05), iii) pre-exposure
to nocodazole decreased the cytotoxicity on cancerous cells (p<0.05) whereas
it did not affect it for the immortalized cells  (p>0.05), iv) in all conditions studied, caspase-3 mRNA levels were analogous
to the control (p>0.05). 

Keywords

Mesothelioma Capsaicin Aphidicolin Nocodazole Cytotoxicity

References

  • Arooz T, Yam CH, Siu WY, Lau A, Li KKW, Poon RYC 2000. On the Concentrations of Cyclins and Cyclin-Dependent Kinases in Extracts of Cultured Human Cells. Biochemistry, 39(31): 9494-9501.
  • Borel F, Lacroix FB, Margolis RL 2002. Prolonged Arrest of Mammalian Cells at the G1/S Boundary Results in Permanent S Phase Stasis. Journal of Cell Science 115: 2829-2838.
  • Cao S, Chen H, Xiang S, Hong J, Weng L, Zhu H, Liu Q 2015. Anti-Cancer Effects and Mechanisms of Capsaicin in Chili Peppers. American Journal of Plant Sciences, 06(19): 3075-3081.
  • Carbone M, Emri S, Dogan AU, Steele I, Tuncer M, Pass HI, Baris YI 2007. A Mesothelioma Epidemic in Cappadocia: Scientific Developments and Unexpected Social Outcomes. Nat Rev Cancer, 7(2): 147-154.
  • Carbone M, Kanodia S, Chao A, Miller A, Wali A, Weissman D, Adjei A, Baumann F, Boffetta P, Buck B, de Perrot M, Dogan AU, Gavett S, Gualtieri A, Hassan R, Hesdorffer M, Hirsch FR, Larson D, Mao W, Masten S, Pass HI, Peto J, Pira E, Steele I, Tsao A, Woodard GA, Yang H, Malik S 2016. Consensus Report of the 2015 Weinman International Conference on Mesothelioma. J Thorac Oncol, 11(8): 1246-1262.
  • Carbone M,Baris YI, Bertino P, Brass B, Comertpay S, Dogan A.U, Gaudino G, Jube S, Kanodia S, Partridge CR, Pass HI, Rivera ZS, Steele I, Tuncer M, Way S, Yang H, Aubrey M 2011. Erionite Exposure in North Dakota and Turkish Villages with Mesothelioma. PNAS, 108(33): 13618–13623.
  • Chow J, Norng M, Zhang J, Chai J 2007. TRPV6 Mediates Capsaicin-Induced Apoptosis in Gastric Cancer Cells—Mechanisms Behind A Possible New “Hot” Cancer Treatment. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1773(4): 565-576.
  • Clark R, Lee SH 2016. Anticancer Properties of Capsaicin Against Human Cancer. Anticancer Research, 36: 837-844.
  • Elmore S 2007. Apoptosis: A Review of Programmed Cell Death. Toxicol Pathol, 35(4): 495-516.
  • Emri SA 2017. The Cappadocia Mesothelioma Epidemic: Its Influence in Turkey and Abroad. Ann Transl Med, 5(11):239.
  • Emri S, Demir A, Dogan M, Akay H, Bozkurt B, Carbone M, Baris I 2002. Lung Diseases Due to Environmental Exposures to Erionite and Asbestos in Turkey. Toxicology Letters, 127(1–3): 251-257.
  • Haltiwanger RS, Philipsberg GA 1997. Mitotic Arrest with Nocodazole Induces Selective Changes in the Level of O-Linked N-Acetylglucosamine and Accumulation of Incompletely Processed N-Glycans on Proteins from HT29 Cells. The journal of biological chemistry, 272(13): 8752–8758.
  • Harashima H, Dissmeyer N, Schnittger A 2013. Cell Cycle Control Across the Eukaryotic Kingdom. Trends Cell Biol, 23(7): 345-356.
  • Helvacı N, Comertpay S 2018. in vitro Evaluation of The Effects of Capsaicin on Normal And Cancerous Cells of Human Cartilage. Turk J Biol, 42: 422-434.
  • Hiddinga BI, Rolfo C, van Meerbeeck JP 2015. Mesothelioma Treatment: Are We on Target? A review. J Adv Res, 6(3): 319-330.
  • Hochegger H, Takeda S, Hunt T 2008. Cyclin-Dependent Kinases and Cell-Cycle Transitions: Does One Fit All? Molecular Cell Biology, 9(11): 910-916.
  • Ho YS, Duh JS , Jeng JH, Wang YJ, Liang YC , Lin CH, Tseng CJ, Yu CF , Chen RJ, Lin JK 2001. Griseofulvin Potentiates Antitumorigenesis Effects of Nocodazole Through Induction of Apoptosis and G2/M Cell Cycle Arrest in Human Colorectal Cancer Cells. Int. J. Cancer 91: 393–401.
  • Holmes RE, Leddy LR 2014. Chondrosarcoma of Bone. Cancer Treat Res, 162: 117-130.
  • Huang SP, Chen JC, Wu CC, Chen CT, Tang NY, Ho YT, Lo C, Lin JP, Chung JG, Lin JG 2009. Capsaicin-induced Apoptosis in Human Hepatoma HepG2 Cells. Anticancer Research, 29: 165-174.
  • Igney FH, Krammer PH 2002. Death And Anti-Death: Tumour Resistance to Apoptosis. Nat Rev Cancer, 2(4): 277-288.
  • Jin T, Wu H, Wang Y, Peng H 2016. Capsaicin Induces Immunogenic Cell Death in Human Osteosarcoma Cells. Exp Ther Med, 12(2): 765-770.
  • Li H, Krstin S, Wang S, Wink M 2018. Capsaicin and Piperine Can Overcome Multidrug Resistance in Cancer Cells to Doxorubicin. Molecules, 23(3): 557.
  • Lowe SW, Lin AW 2000. Apoptosis in Cancer. Carcinogenesis, 21: 485–495.
  • Martinvalet D, Zhu P, Lieberman J 2005. Granzyme A Induces Caspase-Independent Mitochondrial Damage, A Required First Step For Apoptosis. Immunity, 22(3): 355-370.
  • Mori A, Lehmann S, O’Kelly J, Kumagai T, Desmond JC, Pervan M, McBride WH, Kizaki M, Koeffler H P 2006. Capsaicin, a Component of Red Peppers, Inhibits the Growth of Androgen-Independent, p53 Mutant Prostate Cancer Cells. Cancer Research, 66(6): 3222-3229
  • Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N 2013. Crosstalk Between Apoptosis, Necrosis and Autophagy. Biochim Biophys Acta, 1833(12): 3448-3459.
  • Poxleitner MK, Dawson SC, Cande WZ 2008. Cell Cycle Synchrony in Giardia intestinalis Cultures Achieved by Using Nocodazole and Aphidicolin. Eukaryot Cell, 7(4): 569-574.
  • Robinson BWS, Musk AW, Lake RA 2005. Malignant Mesothelioma. The Lancet, 366(9483): 397-408.
  • Roskoski JrR 2019. Cyclin-Dependent Protein Serine/Threonine Kinase Inhibitors As Anticancer Drugs. Pharmacological Research, 139:471-488.
  • Sanchez I, Dynlacht BD 2005. New Insights into Cyclins, Cdks, and Cell Cycle Control. Semin Cell Dev Biol, 16(3): 311-321.
  • Sargent JM, Elgie AW, Williamson CJ, Taylor CG 1996. Aphidicolin Markedly Increases the Platinum Sensitivity of Cells from Primary Ovarian Tumours. Britsh Journal of Cancer, 74: 1730-1733.
  • Starczewska E, Beyaert M, Michaux L, Vekemans MC, Saussoy P, Bol V, Echarri AA, Smal C, Neste EVD, Bontemps F 2016. Targeting DNA Repair with Aphidicolin Sensitizes Primary Chronic Lymphocytic Leukemia Cells to Purine Analogs. Oncotarget, 7(25):38367-38379.
  • Swaffer MP, Jones AW, Flynn HR, Snijders AP, Nurse P 2016. CDK Substrate Phosphorylation and Ordering the Cell Cycle. Cell, 167(7): 1750-1761.
  • Teixeira LK, Reed SI 2017. Cyclin E Deregulation and Genomic Instability. In: Masai H, Foiani M, Editors. DNA Replication: From Old Principles to New Discoveries. 1st ed. Singapore: Springer, pp. 527-547.
  • Truman AW, Kitazono AA, Fitz Gerald JN, Kron SJ 2012. Cell Cycle: Regulation by Cyclins. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net. (Erişim tarihi:11.12.18).
  • Vermeulen K, Bockstaele DRV, Berneman ZN 2003. The Cell Cycle: A Review of Regulation, Deregulation and Therapeutic Targets in Cancer. Cell Prolif., 36: 131–149.
  • Wilding JL, Bodmer WF 2014. Cancer Cell Lines For Drug Discovery and Development. Cancer Res, 74(9): 2377-2384.
  • Ye J, Zhang R, Wu F, Zhai L, Wang K, Xiao M, Xie T, Sui X 2018. Non-Apoptotic Cell Death in Malignant Tumor Cells and Natural Compounds. Cancer Lett, 420: 210-227.
  • Zhang R, Humphreys I, Sahu PR, Shi Y, Srivastava SK 2008. in vitro and in vivo Induction of Apoptosis by Capsaicin in Pancreatic Cancer Cells is Mediated Through ROS Generation and Mitochondrial Death Pathway. Apoptosis, 13(12):1465-1478.
There are 39 citations in total.

Details

Primary Language Turkish
Journal Section RESEARCH ARTICLE
Authors

Sabahattin Cömertpay 0000-0003-4850-6927

Gizem Ceylan 0000-0001-6281-4665

Publication Date June 30, 2019
Submission Date December 12, 2018
Acceptance Date February 15, 2019
Published in Issue Year 2019Volume: 22 Issue: 3

Cite

APA Cömertpay, S., & Ceylan, G. (2019). Öncül Afidikolin ve Nokodazol Muamelesinin Kanserli ve Ölümsüzleştirilmiş Mezotelyum Hücrelerinde Kapsaisin Sitotoksitesine Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22(3), 456-465. https://doi.org/10.18016/ksutarimdoga.vi.496108

Cited By

Kapsaisinin H2452 Mezotelyoma Hücre Hattından Seçilen Klonlarda Sitotoksisitesi ve Kaspaz-3 Üzerine Etkilerinin Belirlenmesi
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
Gizem CEYLAN
https://doi.org/10.18016/ksutarimdoga.vi.673591
 Download Cover Image


International Peer Reviewed Journal
Free submission and publication
Published 6 times a year



88x31.png


KSU Journal of Agriculture and Nature

e-ISSN: 2619-9149