Year 2019, Volume 22 , Issue 3, Pages 473 - 479 2019-06-30

Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells
Dorycnium pentaphyllum Ekstraktının İnsan Meme, Karaciğer ve Akciğer Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Selim DEMİR [1] , İbrahim TURAN [2] , Sema MISIR [3] , Yüksel ALİYAZICIOĞLU [4]


Although some studies have evaluated the cytotoxic activity of different Fabaceae species, there has been only limited research into the cytotoxic effect of Dorycnium pentaphyllum. The purpose of this study was to determine the antioxidant properties and cytotoxic effect of D. pentaphyllum extract on various human cancer cells. The total phenolic content (TPC) of the extract was determined using colorimetric method. The cytotoxic effect of the extract on human lung (A549), liver (HepG2), and breast (MCF-7) cancer cells and a normal human fibroblast cells was assessed using the MTT assay. TPC value of extract was found as 140.3±1.1 mg gallic acid equivalent per g sample. Extract showed selective cytotoxicity on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cells range from 100.4 to 298.5 μg/mL. This is the first study to reveal the cytotoxic effect of D. pentaphyllum extract on cancer cell lines. Phytomedical applications of D. pentaphyllum may represent promising approaches in the treatment of cancer.

Fabaceae ailesine mensup bitkilerin sitotoksik etkilerini konu alan bazı çalışmalar bulunmakla birlikte, Dorycnium pentaphyllum'un sitotoksik etkisi ile ilgili sınırlı araştırma bulunmaktadır. Bu çalışmanın amacı, D. pentaphyllum ekstraktının antioksidan özelliklerinin ve çeşitli insan kanser hücreleri üzerindeki sitotoksik etkisini belirlenmesidir. Ekstraktın toplam fenolik içeriği kolorimetrik yöntem kullanılarak belirlendi. Ekstraktın insan akciğer (A549), karaciğer (HepG2) ve meme (MCF-7) kanser hücreleri ile normal insan fibroblast hücreleri üzerindeki sitotoksik etkisi MTT analizi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içerik değeri g örnek başına 140.3±1.1 mg gallik asit eşdeğeri olarak bulundu. Ekstraktın çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre seçici sitotoksik etki gösterdiği belirlendi ve IC50 değerleri 100.4-298.5 μg/mL arasında hesaplandı. Bu çalışma D. pentaphyllum ekstraktının kanser hücre serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. D. pentaphyllum'un fitokimyasal uygulamaları kanser tedavisinde umut verici yaklaşımları temsil edebilir.

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Primary Language en
Subjects Science
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-1863-6280
Author: Selim DEMİR (Primary Author)
Institution: Karadeniz Technical University
Country: Turkey


Orcid: 0000-0003-3400-5494
Author: İbrahim TURAN
Country: Turkey


Orcid: 0000-0003-3400-5588
Author: Sema MISIR
Institution: CUMHURIYET UNIVERSITY
Country: Turkey


Orcid: 0000-0001-9474-4307
Author: Yüksel ALİYAZICIOĞLU
Institution: Karadeniz Technical University
Country: Turkey


Dates

Application Date : December 15, 2018
Acceptance Date : February 4, 2019
Publication Date : June 30, 2019

APA Demi̇r, S , Turan, İ , Mısır, S , Ali̇yazıcıoğlu, Y . (2019). Selective Cytotoxic Effect of Dorycnium pentaphyllum Extract on Human Breast, Liver, and Lung Cancer Cells . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 (3) , 473-479 . DOI: 10.18016/ksutarimdoga.vi.497868