Chemical Content and Biological Activity Spectrum of Nigella sativa Seed Oil

Yusuf Sıcak; Elif Ayşe Erdoğan Eliuz

doi:10.18016/ksutarimdoga.vi.537674

Research Article

Chemical Content and Biological Activity Spectrum of Nigella sativa Seed Oil

Year 2019, Volume: 22 Issue: 6, 928 - 934, 31.12.2019

Yusuf Sıcak , Elif Ayşe Erdoğan Eliuz

https://doi.org/10.18016/ksutarimdoga.vi.537674

Cited By: 15

Abstract

In this study, it was aimed
to investigate the chemical composition and biological activities including
antioxidant, anticholinesterase, antimicrobial and urease activity of Nigella sativa seed oil growing in Muğla
(Köyceğiz). The oil of plant was obtained using Soxhlet apparatus. Thirty-one
components of the oil were determined by Gas Chromatography-Mass Spectrometry
(GC-MS). The major components of oil were detected as palmitic acid (10.48%),
linoleic acid (8.05%), o-cyemene
(7.11%), 3,5-dimethyl cyclohexanol (6.68%), thymoquinone (6.44%), p-tert-buthyl catechol (6.28%) and
8-methyl-1-undecene (3.28%). The findings obtained from biological activity
assays showed that Nigella sativa oil
was a promising candidate that can be used in the discovery of new drugs and
the preparation of new natural drug preparations.

Keywords

Nigella sativa, Chemical Composition, Biological Activity

Supporting Institution

Muğla Sıtkı Koçman University Research Fund

Project Number

Project Number: 17/216

Thanks

The authors would like to thank the Muğla Sıtkı Koçman University Research Fund (Project Number: 17/216) for the financial support of the antioxidant and anticholinesterase activities in this research project.

References

Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. 2013. A Review on Therapeutic Potential of Nigella sativa: A Miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5): 337-352.
Ali-Shtayeh MS, Jamous RM, Abu-Zaitoun SY, Akkawi RJ, Kalbouneh SR, Bernstein N, Dudai N. 2018. Chemical Profile and Bioactive Properties of The Essential Oil Isolated from Clinopodium serpyllifolium (M.Bieb.) Kuntze Growing in Palestine. Industrial Crops and Products, 124: 617-625. Al-Naqeep G, Al-Zubairi AS, Ismail M, Amom ZH, Esa NM. 2011. Antiatherogenic Potential of Nigella sativa Seeds and Oil in Diet-Induced Hypercholesterolemia in Rabbits. Evidence-Based Caomplementary and Alternative Medicine, 2011: 1-8.
Apak R, Güçlü K, Özyürek M, Karademir SE. 2004. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capacity In The Presence of Neocuproine: Cuprac Method. Journal of Agricultural and Food Chemistry, 52(26): 7970-7981.
Blois MS. 1958. Antioxidant Determinations by The Use of A Stable Free Radical. Nature, 181: 1199-1200.
Bourgou, S., Pichette, A., Marzouk, B., Legault, J. 2010. Bioactivities of black cumin essential oil and its main terpenes from Tunisia. South African Journal of Botany, 76(2): 210-216.
Burits M, Bucar F. 2000. Antioxidant Activity of Nigella sativa Essential Oil. Phytotherapy Research, 14(5): 323-328.
Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. 2011. Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement and Alternative Medicine, 11(29): 1-6.
Ellman GL, Courtney KD, Andres V, Featherstone RM. 1961. A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity. Biochemistry Pharmacology Behavior, 7(2): 88-95.
Erdoğan Eliuz EA, Ayaş D, Gökşen G. 2017. In vitro Phototoxicity and Antimicrobial Activity of Volatile Oil Obtained from Aromatic Plants. Journal of Essential Oil Bearing Plants, 20(3): 758-768.
Ferdous AJ, Islam SN, Ahsan M, Hasan CM, Ahmed ZU. 1992. In vitro Antibacterial Activity of the Volatile Oil of Nigella sativa Seeds Against Multiple Drug Resistance Isolated of Shigella spp. and Isolated of Vibrio chloerae and Isolated of Escherichia coli. Phytotherapy Research, 6(3): 137-140.
Forouzanfar F, Fazly Bazzaz BS, Hosseinzadeh H. 2014. Black Cumin (Nigella sativa) and Its Constituent (Thymoquinone): A Review on Antimicrobial Effects. Iranian Journal of Basic Medical Sciences, 17(12): 929-938.
Gharby S, Harhar H, Guillaume D, Roudani A, Boulbaroud S, Ibrahimi M, Ahmad M, Sultana S, Hadda TB, Chafchaouni-Moussaoui I, Charrouf Z. 2015. Chemical Investigations of Nigella sativa L. Seed Oil Produced in Morocco. Journal of the Saudi Society of Agricultural Sciences, 14(2): 172-177.
Hanafy MS, Hatem ME. 1991. Studies on the Antimicrobial Activity of Nigella sativa Seed (Black Cumin). Journal of Ethnopharmacology, 34(2-3): 275-278.
Hannan A, Saleem S, Chaudhary S, Barkaat M, Arshad MU. 2008. Anti-bacterial activity of Nigella sativa Against Clinical Isolates of Methicillin Resistant Staphylococcus aureus. Journal of Ayub Medical College Abbottabad, 20(3): 72-74.
Hosseinzadeh H, Fazly Bazzaz BS, Motevaly Haghi M. 2007. Antibacterial Activity of Total Extracts and Essential Oil of Nigella sativa L. Seeds in Mice. Pharmacology, 2: 429-435.
Hosseinzadeh H, Tafaghodi M, Mosavi J, Taghiabadi E. 2013. Effect of Aqueous and Ethanolic Extracts of Nigella sativa Seeds on Milk Production in Rats. Journal of Acupuncture & Meridian Studies, 6(1): 18-23.
Huseini Fallah A, Amini M, Mohtashami R, Ghamarchehre M, Sadeqhi Z, Kianbakht S, Fallah Huseini A. 2013. Blood Pressure Lowering Effect of Nigella sativa L. Seed Oil in Healthy Volunteers: A Randomized, Double-Blind, Placebo controlled Clinical Trial. Phytotherapy Research, 27(12): 1849-1853.
Islam MT, Guha B, Hosen S, Riaz T A, Shahadat S, Da Rocha Sousa L, De Oliveira Santos J V, Da Silva Júnior J J, De Lima R M T, Braga A L, Dos Reis A C, De Alencar MVOB, De Carvalho Melo-Cavalcante A A, 2017. Nigellalogy: A Review on Nigella Sativa, MOJ Bioequivalance and Bioavailability, 3(6): 00056
Kannan R, Sibi P, Jyoti Harindran I. 2019. Acetylcholinesterase and Growth Inhibitory Effects-Various Grades of N. Sativa Oils, International Journal of Pharmaceutical Sciences and Research, 10(1): 245-250.
Le P, Benhaddou-Andaloussi A, Elimadi A, Settaf A, Cherrah Y, Haddad P. 2004. The Petroleum Ether Extracts of Nigella sativa Seeds Exert Lipid-lowering and Insulin Sensitizing Action in Rats. Journal of Etnopharmaocolgy, 94(2-3): 251-259.
Llorent-Martinez EJ, Zengin G, Ortega-Barrales P, Aumeeruddy MZ, Locatelli M, Mollica A, Mahomoodally MF. 2018. Characterization of The Phytochemical Profiles and Biological Activities of Ajuga chamaepitys subsp. Chia var. chia and Ajuga bombycina by High-Performance Liquid Chromatography-Electrosray Ionization-Tandem Mass Spectrometry (HPLC-ESI-MSn), Analytical Letters, DOI: 10.1080/00032719.2018.1500581.
Marco GJ. 1968. A Rapid Method for Evaluation of Antioxidants. Journal of The American Oil Chemists’ Society, 45(9): 594-598.
McFarland J. 1987. Standardization Bacteria Culture for the Disc Diffusion Assay. The Journal of American Medical Association, 49(14): 1176-1178.
Nair MKM, Vasudevan P, Venkitanarayanan K. 2005. Antibacterial Effect of Black Seed Oil on Listeria Monocytogenes. Food Control, 16(5): 395-398.
Najmi A, Haque S, Naseeruddin M, Khan R. 2008. Effect of Nigella sativa Oil on Various Clinical and Biochemical Parameters of Metabolic Syndrome. Intertional Journal of Diabetes in Developing Coutries, 28(1): 11-14.
Noor NA, Aboul Ezz HS, Faraag A, Khadrawy YA. 2012. Evaluation of the Antiepileptic Effect of Curcumin and Nigella sativa Oil in the Pilocarpine Model of Epilepsy in Comparison with Valproate. Epilepys & Behavior, 24(2): 199-206.
Öztürk M, Kolak U, Topçu G, Öksüz S, Chourdhary MI. 2011. Antioxidant and Anticholinestrease Active Constituents from Micromeria cilicica by Radical-Scavenging Activity-Guided Fractionation. Food Chemistry, 126(1): 31-38.
Patton T, Barett J, Brennan J, Moran N. 2006. Use of a Spectrophotometric Bioassay for Determination of Microbial Sensitivity to Manuka Honey. Journal of Microbiological Methods, 64(1):84-95.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1989. Antioxidant Activity Applying an Improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26(9-10): 1231-1237.
Salem ML. 2005. Immunomodulatory ant therapeutic properties of Nigella sativa L. seed. International Immunopharmacology, 5(13-14): 1749-1770.
Venkatachallam SKT, Pattekhan H, Divakar S, Kadimi US. 2010. Chemical Composition of Nigella sativa L. Seed Extracts Obtained by Supercritical Carbondioxide. Journal of Food Science and Technology, 47(6): 598-605.
Weatherburn MW. 1967. Phenol-hypochlorite Reaction for Determination of Ammonia. Analytical Chemistry, 3: 971-974.

Çörek Otu Tohumu Yağının Kimyasal İçeriği ve Biyolojik Aktivite Spektrumu

Year 2019, Volume: 22 Issue: 6, 928 - 934, 31.12.2019

Yusuf Sıcak , Elif Ayşe Erdoğan Eliuz

https://doi.org/10.18016/ksutarimdoga.vi.537674

Cited By: 15

Abstract

Bu çalışmada, Muğla
(Köyceğiz)'de yetişen Nigella sativa
(çörek otu) tohumu yağının kimyasal bileşimi ile yağın antioksidan,
antikolinesteraz, antimikrobiyal ve üreaz aktivitesinin araştırılması
amaçlanmıştır. Bitkinin yağı, Soxhlet aparatı kullanılarak elde edildi. Yağ
bileşimi Gaz Kromatografisi-Kütle Spektrometresi (GC-MS) ile belirlendi. GC-MS
sonuçlarından, yağın otuz bir bileşeni tespit edilebildi. Yağın ana bileşenleri
palmitik asit (% 10.48), linoleik asit (%8.05), o-siemen (% 7.11), 3,5-dimetil
sikloheksanol (% 6.68), timokinon (% 6.44),
p-tert-butil katekol (% 6.28) ve 8-metil-1-undesen (% 3.28) olarak
belirlendi. Biyolojik aktivite test sonuçları tıbbi özelliği olan çörek otu
yağının yeni ilaçların keşfinde ve yeni doğal drog preparatların
hazırlanmasında kullanılabilecek umut verici bir aday olduğunu göstermektedir.

Keywords

Çörek Otu, Kimyasal Bileşen, Biyolojik Aktivite

Project Number

Project Number: 17/216

References

Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. 2013. A Review on Therapeutic Potential of Nigella sativa: A Miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5): 337-352.
Ali-Shtayeh MS, Jamous RM, Abu-Zaitoun SY, Akkawi RJ, Kalbouneh SR, Bernstein N, Dudai N. 2018. Chemical Profile and Bioactive Properties of The Essential Oil Isolated from Clinopodium serpyllifolium (M.Bieb.) Kuntze Growing in Palestine. Industrial Crops and Products, 124: 617-625. Al-Naqeep G, Al-Zubairi AS, Ismail M, Amom ZH, Esa NM. 2011. Antiatherogenic Potential of Nigella sativa Seeds and Oil in Diet-Induced Hypercholesterolemia in Rabbits. Evidence-Based Caomplementary and Alternative Medicine, 2011: 1-8.
Apak R, Güçlü K, Özyürek M, Karademir SE. 2004. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capacity In The Presence of Neocuproine: Cuprac Method. Journal of Agricultural and Food Chemistry, 52(26): 7970-7981.
Blois MS. 1958. Antioxidant Determinations by The Use of A Stable Free Radical. Nature, 181: 1199-1200.
Bourgou, S., Pichette, A., Marzouk, B., Legault, J. 2010. Bioactivities of black cumin essential oil and its main terpenes from Tunisia. South African Journal of Botany, 76(2): 210-216.
Burits M, Bucar F. 2000. Antioxidant Activity of Nigella sativa Essential Oil. Phytotherapy Research, 14(5): 323-328.
Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. 2011. Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement and Alternative Medicine, 11(29): 1-6.
Ellman GL, Courtney KD, Andres V, Featherstone RM. 1961. A New and Rapid Colorimetric Determination of Acetylcholinesterase Activity. Biochemistry Pharmacology Behavior, 7(2): 88-95.
Erdoğan Eliuz EA, Ayaş D, Gökşen G. 2017. In vitro Phototoxicity and Antimicrobial Activity of Volatile Oil Obtained from Aromatic Plants. Journal of Essential Oil Bearing Plants, 20(3): 758-768.
Ferdous AJ, Islam SN, Ahsan M, Hasan CM, Ahmed ZU. 1992. In vitro Antibacterial Activity of the Volatile Oil of Nigella sativa Seeds Against Multiple Drug Resistance Isolated of Shigella spp. and Isolated of Vibrio chloerae and Isolated of Escherichia coli. Phytotherapy Research, 6(3): 137-140.
Forouzanfar F, Fazly Bazzaz BS, Hosseinzadeh H. 2014. Black Cumin (Nigella sativa) and Its Constituent (Thymoquinone): A Review on Antimicrobial Effects. Iranian Journal of Basic Medical Sciences, 17(12): 929-938.
Gharby S, Harhar H, Guillaume D, Roudani A, Boulbaroud S, Ibrahimi M, Ahmad M, Sultana S, Hadda TB, Chafchaouni-Moussaoui I, Charrouf Z. 2015. Chemical Investigations of Nigella sativa L. Seed Oil Produced in Morocco. Journal of the Saudi Society of Agricultural Sciences, 14(2): 172-177.
Hanafy MS, Hatem ME. 1991. Studies on the Antimicrobial Activity of Nigella sativa Seed (Black Cumin). Journal of Ethnopharmacology, 34(2-3): 275-278.
Hannan A, Saleem S, Chaudhary S, Barkaat M, Arshad MU. 2008. Anti-bacterial activity of Nigella sativa Against Clinical Isolates of Methicillin Resistant Staphylococcus aureus. Journal of Ayub Medical College Abbottabad, 20(3): 72-74.
Hosseinzadeh H, Fazly Bazzaz BS, Motevaly Haghi M. 2007. Antibacterial Activity of Total Extracts and Essential Oil of Nigella sativa L. Seeds in Mice. Pharmacology, 2: 429-435.
Hosseinzadeh H, Tafaghodi M, Mosavi J, Taghiabadi E. 2013. Effect of Aqueous and Ethanolic Extracts of Nigella sativa Seeds on Milk Production in Rats. Journal of Acupuncture & Meridian Studies, 6(1): 18-23.
Huseini Fallah A, Amini M, Mohtashami R, Ghamarchehre M, Sadeqhi Z, Kianbakht S, Fallah Huseini A. 2013. Blood Pressure Lowering Effect of Nigella sativa L. Seed Oil in Healthy Volunteers: A Randomized, Double-Blind, Placebo controlled Clinical Trial. Phytotherapy Research, 27(12): 1849-1853.
Islam MT, Guha B, Hosen S, Riaz T A, Shahadat S, Da Rocha Sousa L, De Oliveira Santos J V, Da Silva Júnior J J, De Lima R M T, Braga A L, Dos Reis A C, De Alencar MVOB, De Carvalho Melo-Cavalcante A A, 2017. Nigellalogy: A Review on Nigella Sativa, MOJ Bioequivalance and Bioavailability, 3(6): 00056
Kannan R, Sibi P, Jyoti Harindran I. 2019. Acetylcholinesterase and Growth Inhibitory Effects-Various Grades of N. Sativa Oils, International Journal of Pharmaceutical Sciences and Research, 10(1): 245-250.
Le P, Benhaddou-Andaloussi A, Elimadi A, Settaf A, Cherrah Y, Haddad P. 2004. The Petroleum Ether Extracts of Nigella sativa Seeds Exert Lipid-lowering and Insulin Sensitizing Action in Rats. Journal of Etnopharmaocolgy, 94(2-3): 251-259.
Llorent-Martinez EJ, Zengin G, Ortega-Barrales P, Aumeeruddy MZ, Locatelli M, Mollica A, Mahomoodally MF. 2018. Characterization of The Phytochemical Profiles and Biological Activities of Ajuga chamaepitys subsp. Chia var. chia and Ajuga bombycina by High-Performance Liquid Chromatography-Electrosray Ionization-Tandem Mass Spectrometry (HPLC-ESI-MSn), Analytical Letters, DOI: 10.1080/00032719.2018.1500581.
Marco GJ. 1968. A Rapid Method for Evaluation of Antioxidants. Journal of The American Oil Chemists’ Society, 45(9): 594-598.
McFarland J. 1987. Standardization Bacteria Culture for the Disc Diffusion Assay. The Journal of American Medical Association, 49(14): 1176-1178.
Nair MKM, Vasudevan P, Venkitanarayanan K. 2005. Antibacterial Effect of Black Seed Oil on Listeria Monocytogenes. Food Control, 16(5): 395-398.
Najmi A, Haque S, Naseeruddin M, Khan R. 2008. Effect of Nigella sativa Oil on Various Clinical and Biochemical Parameters of Metabolic Syndrome. Intertional Journal of Diabetes in Developing Coutries, 28(1): 11-14.
Noor NA, Aboul Ezz HS, Faraag A, Khadrawy YA. 2012. Evaluation of the Antiepileptic Effect of Curcumin and Nigella sativa Oil in the Pilocarpine Model of Epilepsy in Comparison with Valproate. Epilepys & Behavior, 24(2): 199-206.
Öztürk M, Kolak U, Topçu G, Öksüz S, Chourdhary MI. 2011. Antioxidant and Anticholinestrease Active Constituents from Micromeria cilicica by Radical-Scavenging Activity-Guided Fractionation. Food Chemistry, 126(1): 31-38.
Patton T, Barett J, Brennan J, Moran N. 2006. Use of a Spectrophotometric Bioassay for Determination of Microbial Sensitivity to Manuka Honey. Journal of Microbiological Methods, 64(1):84-95.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1989. Antioxidant Activity Applying an Improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26(9-10): 1231-1237.
Salem ML. 2005. Immunomodulatory ant therapeutic properties of Nigella sativa L. seed. International Immunopharmacology, 5(13-14): 1749-1770.
Venkatachallam SKT, Pattekhan H, Divakar S, Kadimi US. 2010. Chemical Composition of Nigella sativa L. Seed Extracts Obtained by Supercritical Carbondioxide. Journal of Food Science and Technology, 47(6): 598-605.
Weatherburn MW. 1967. Phenol-hypochlorite Reaction for Determination of Ammonia. Analytical Chemistry, 3: 971-974.

There are 32 citations in total.

Details

Primary Language	English
Subjects	Structural Biology
Journal Section	RESEARCH ARTICLE
Authors	Yusuf Sıcak 0000-0003-2339-5837 Elif Ayşe Erdoğan Eliuz 0000-0003-4317-3000
Project Number	Project Number: 17/216
Publication Date	December 31, 2019
Submission Date	March 9, 2019
Acceptance Date	May 16, 2019
Published in Issue	Year 2019Volume: 22 Issue: 6

Cite

APA	Sıcak, Y., & Erdoğan Eliuz, E. A. (2019). Chemical Content and Biological Activity Spectrum of Nigella sativa Seed Oil. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22(6), 928-934. https://doi.org/10.18016/ksutarimdoga.vi.537674