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In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)

Year 2020, Volume: 23 Issue: 4, 870 - 875, 31.08.2020

Pelin Gökçe , Süleyman Avcı

https://doi.org/10.18016/ksutarimdoga.vi.657729

Abstract

To investigate the regeneration potential of Lotus maritimus L., the stem, hypocotyl, cotyledon, root, and epicotyl were cultured in MS media containing different combinations of α-naphthalene acetic acid (NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0 (control), 1 mg L-1 of 6-Benzilaminopurin (BAP), and 0.5 mg L-1 of kinetin]. The highest callus induction was achieved with the stem and epicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mg L-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shoots per callus) were determined with the epicotyl and hypocotyl cultured in MS media containing 1 mg L-1 of NAA. The obtained shoots were easily rooted in half-strength MS media containing 1 mg L-1 NAA and substantially adapted to the external environment.

Keywords

NAA concentrations Epicotyl Hypocotyl Callus induction Kinetin

Thanks

This article is based on Pelin GÖKÇE’s master thesis entitled with "Determination of in vitro regeneration ability of Lotus maritimus L."

References

  • Akashi R, Hoffmann-Tsay SS, Hoffmann F 1998. Selection of a Super-Growing Legume Root Culture that Permits Controlled Switching between Root Cloning and Direct Embryogenesis. Theoretical and Applied Genetics, 96 (6-7): 758–764. https://doi.org/10.1007/s001220050799.
  • Arambarri AM, Sebastian C, Stenglein A, Colares MN, Novoa MC 2005. Taxonomy of the New World Species of Lotus (Leguminosae: Loteae). Australian Journal of Botany, 53: 797–812. DOI: 10.1071/BT04101.
  • Díaz P, Borsani O, Monza J 2005. Lotus japonicus Related Species and Their Agronomic Importance. (Lotus japonicus Handbook, Springer, Netherlands: Ed. Marquez AJ, 25-27.
  • Escaray FJ, Menendez AB, Gárriz A, Pieckenstain FL, Estrella MJ, Castagno LN, Carrasco P, Sanjuán J, Ruiz OA 2012. Ecological and Agronomic Importance of the Plant Genus Lotus. Its Application in Grassland Sustainability and the Amelioration of Constrained and Contaminated Soils. Plant Science, 182: 121-133. https://doi.org/10.1016/j.plantsci.2011.03.016.
  • Espasandin FD, Collavino MM, Luna CV, Paz RC, Tarrago JR, Ruiz OA, Mroginski LA, Sansberro PA 2010. Agrobacterium tumefaciens-Mediated Transformation of Lotus tenuis and Regeneration of Transgenic Lines. Plant Cell Tissue Organ Culture, 102: 181–189. DOI: 10.1007/s11240-010-9720-x.
  • George RAT 2012. Agricultural Seed Production. Cabi Publishing, Oxfordshire, UK. pp. 216.
  • Handberg K, Stougaard J 1992. Lotus japonicus, an Autogamous, Diploid Legume Species for Classical and Molecular Genetics. The Plant Journal, 2 (4): 487-496. https://doi.org/10.1111/j.1365-313X.1992.00487.x.
  • Heyn CC 1970. Tetragonolobus Scop. (Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh: Ed. Davis PH) Volume 3: 531.
  • Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology, 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962. tb08052.x.
  • Kavak S 2014. Tetragonolobus maritimus. The IUCN Red List of Threatened Species 2014: e.T19619442A19621026. http://dx.doi.org/10. 2305/IUCN.UK.2014-1.RLTS.T19619442A1962 1026.en.
  • Lombari P, Ercolano, El Alaoui H, Chiurazzi M 2003. A New Transformation-Regeneration Procedure in the Model Legume Lotus japonicus: Root Explants as a Source of Large Numbers of Cells Susceptible to Agrobacterium-mediated Transformation. Plant Cell Reports , 21 (8): 771–777. https://doi.org/10.1007/s00299-003-0576-y.
  • Nenz E, Pupilli F, Paolocci F, Damiani F, Cenci CA, Arcioni S 1996. Plant Regeneration and Genetic Transformation of Lotus angustissimus. Plant Cell, Tissue and Organ Culture, 45(2): 145–152. https://doi.org/10.1007/BF00048758.
  • Nikolić R, Mitić N, Miletić R, Nešković M 2006. Effects of Cytokinins on In Vitro Seed Germination and Early Seedling Morphogenesis in Lotus corniculatus L. Journal of Plant Growth Regulation, 25(3):187-194. DOI: 10.1007/s00344-005-0129-4.
  • Orcen N 2013. Regeneration of Bird's-Foot Trefoil (Lotus corniculatus L.) Native Race of Aegean Region. Fresenius Environmental Bulletin, 22(8a):2409-2414.
  • Piccirilli M, Pupilli F, Arcioni S 1988. Lotus tenuis Wald.& Kit.: In Vitro Conditions for Plant Regeneration from Protoplasts and Callus of Various Explants. Plant Science, 55 (1): 77-82.
  • Pupilli F, Arcioni S, Damiani F, Pezzotti M 1990. Plant Regeneration from Callus and Protoplast Cultures of Lotus pedunculatus Cav. Plant Cell, Tissue and Organ Culture , 23 (3): 193–199. https://doi.org/10.1007/BF00034431.
  • Roskov YR, Bisby FA, Zarucchi JL, Schrire BD, White RJ 2006. ILDIS World Database of Legumes: Draft Checklist, Version 10 [published June, but CD shows November 2005 date]. ILDIS, Reading, UK, [CD-Rom: ISBN 0 7049 1248 1].
  • Sokoloff DD, Lock JM 2005. Loteae. (Legumes of the World. Bath Press, UK: Ed. Lewis G, Schrire B, Mackinder B, Lock M) 455-466.
  • Suman S 2017. Plant Tissue Culture: A promising Tool of Quality Material Production with Special Reference to Micropropagation of Banana. Biochemical and Cellular Archives, 17 (1): 1-26.

Lotus maritimus L.'nin (Leguminosae) in Vitro Rejenerasyon Potansiyeli

Year 2020, Volume: 23 Issue: 4, 870 - 875, 31.08.2020

Pelin Gökçe , Süleyman Avcı

https://doi.org/10.18016/ksutarimdoga.vi.657729

Abstract

Lotus maritimus L.'nin rejenerasyon potansiyelini araştırmak için, L. maritimus'un tohumları in vitro koşullarda ekilmiş ve daha sonra kök, hipokotil, kotiledon, sap ve epikotil parçaları, farklı α-naphthalene acetic acid (NAA) konsantrasyonları (1, 2 ve 4 mg L-1) ve sitokinin tiplerinin [0 (kontrol), 1 mg L-1 6-Benzilaminopurin (BAP) ve 0.5 mg L-1 kinetin] kombinasyonlarını içeren MS ortamlarında kültüre alınmıştır. Sonuçlara göre, en yüksek kallus indüksiyonu, sap ve epikotil eksplantlarından 1 veya 2 mg L-1 NAA konsantrasyonu + 0.5 mg L-1 kinetin ile sağlanmıştır. En iyi bitki rejenerasyonu değerleri (3.56 ve 3.20 sürgün/kallus) ise epikotil ve hipokotil parçalarının 1 mg L-1 NAA içeren MS ortamında kültüre alınmasıyla elde edilmiştir. Oluşan sürgünler, 1 mg L-1 NAA içeren ½ MS ortamında kolayca köklendirilmiş ve büyük ölçüde dış ortama aktarılmıştır.

Keywords

NAA konsantrasyonları Epikotil Hipokotil Kallus oluşumu Kinetin

References

  • Akashi R, Hoffmann-Tsay SS, Hoffmann F 1998. Selection of a Super-Growing Legume Root Culture that Permits Controlled Switching between Root Cloning and Direct Embryogenesis. Theoretical and Applied Genetics, 96 (6-7): 758–764. https://doi.org/10.1007/s001220050799.
  • Arambarri AM, Sebastian C, Stenglein A, Colares MN, Novoa MC 2005. Taxonomy of the New World Species of Lotus (Leguminosae: Loteae). Australian Journal of Botany, 53: 797–812. DOI: 10.1071/BT04101.
  • Díaz P, Borsani O, Monza J 2005. Lotus japonicus Related Species and Their Agronomic Importance. (Lotus japonicus Handbook, Springer, Netherlands: Ed. Marquez AJ, 25-27.
  • Escaray FJ, Menendez AB, Gárriz A, Pieckenstain FL, Estrella MJ, Castagno LN, Carrasco P, Sanjuán J, Ruiz OA 2012. Ecological and Agronomic Importance of the Plant Genus Lotus. Its Application in Grassland Sustainability and the Amelioration of Constrained and Contaminated Soils. Plant Science, 182: 121-133. https://doi.org/10.1016/j.plantsci.2011.03.016.
  • Espasandin FD, Collavino MM, Luna CV, Paz RC, Tarrago JR, Ruiz OA, Mroginski LA, Sansberro PA 2010. Agrobacterium tumefaciens-Mediated Transformation of Lotus tenuis and Regeneration of Transgenic Lines. Plant Cell Tissue Organ Culture, 102: 181–189. DOI: 10.1007/s11240-010-9720-x.
  • George RAT 2012. Agricultural Seed Production. Cabi Publishing, Oxfordshire, UK. pp. 216.
  • Handberg K, Stougaard J 1992. Lotus japonicus, an Autogamous, Diploid Legume Species for Classical and Molecular Genetics. The Plant Journal, 2 (4): 487-496. https://doi.org/10.1111/j.1365-313X.1992.00487.x.
  • Heyn CC 1970. Tetragonolobus Scop. (Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh: Ed. Davis PH) Volume 3: 531.
  • Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology, 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962. tb08052.x.
  • Kavak S 2014. Tetragonolobus maritimus. The IUCN Red List of Threatened Species 2014: e.T19619442A19621026. http://dx.doi.org/10. 2305/IUCN.UK.2014-1.RLTS.T19619442A1962 1026.en.
  • Lombari P, Ercolano, El Alaoui H, Chiurazzi M 2003. A New Transformation-Regeneration Procedure in the Model Legume Lotus japonicus: Root Explants as a Source of Large Numbers of Cells Susceptible to Agrobacterium-mediated Transformation. Plant Cell Reports , 21 (8): 771–777. https://doi.org/10.1007/s00299-003-0576-y.
  • Nenz E, Pupilli F, Paolocci F, Damiani F, Cenci CA, Arcioni S 1996. Plant Regeneration and Genetic Transformation of Lotus angustissimus. Plant Cell, Tissue and Organ Culture, 45(2): 145–152. https://doi.org/10.1007/BF00048758.
  • Nikolić R, Mitić N, Miletić R, Nešković M 2006. Effects of Cytokinins on In Vitro Seed Germination and Early Seedling Morphogenesis in Lotus corniculatus L. Journal of Plant Growth Regulation, 25(3):187-194. DOI: 10.1007/s00344-005-0129-4.
  • Orcen N 2013. Regeneration of Bird's-Foot Trefoil (Lotus corniculatus L.) Native Race of Aegean Region. Fresenius Environmental Bulletin, 22(8a):2409-2414.
  • Piccirilli M, Pupilli F, Arcioni S 1988. Lotus tenuis Wald.& Kit.: In Vitro Conditions for Plant Regeneration from Protoplasts and Callus of Various Explants. Plant Science, 55 (1): 77-82.
  • Pupilli F, Arcioni S, Damiani F, Pezzotti M 1990. Plant Regeneration from Callus and Protoplast Cultures of Lotus pedunculatus Cav. Plant Cell, Tissue and Organ Culture , 23 (3): 193–199. https://doi.org/10.1007/BF00034431.
  • Roskov YR, Bisby FA, Zarucchi JL, Schrire BD, White RJ 2006. ILDIS World Database of Legumes: Draft Checklist, Version 10 [published June, but CD shows November 2005 date]. ILDIS, Reading, UK, [CD-Rom: ISBN 0 7049 1248 1].
  • Sokoloff DD, Lock JM 2005. Loteae. (Legumes of the World. Bath Press, UK: Ed. Lewis G, Schrire B, Mackinder B, Lock M) 455-466.
  • Suman S 2017. Plant Tissue Culture: A promising Tool of Quality Material Production with Special Reference to Micropropagation of Banana. Biochemical and Cellular Archives, 17 (1): 1-26.
There are 19 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section RESEARCH ARTICLE
Authors

Pelin Gökçe 0000-0002-3865-059X

Süleyman Avcı 0000-0002-4653-5567

Publication Date August 31, 2020
Submission Date December 10, 2019
Acceptance Date March 17, 2020
Published in Issue Year 2020Volume: 23 Issue: 4

Cite

APA Gökçe, P., & Avcı, S. (2020). In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(4), 870-875. https://doi.org/10.18016/ksutarimdoga.vi.657729
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