Year 2020, Volume 23 , Issue 4, Pages 870 - 875 2020-08-31

Lotus maritimus L.'nin (Leguminosae) in Vitro Rejenerasyon Potansiyeli
In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae)

Pelin GÖKÇE [1] , Süleyman AVCI [2]


Lotus maritimus L.'nin rejenerasyon potansiyelini araştırmak için, L. maritimus'un tohumları in vitro koşullarda ekilmiş ve daha sonra kök, hipokotil, kotiledon, sap ve epikotil parçaları, farklı α-naphthalene acetic acid (NAA) konsantrasyonları (1, 2 ve 4 mg L-1) ve sitokinin tiplerinin [0 (kontrol), 1 mg L-1 6-Benzilaminopurin (BAP) ve 0.5 mg L-1 kinetin] kombinasyonlarını içeren MS ortamlarında kültüre alınmıştır. Sonuçlara göre, en yüksek kallus indüksiyonu, sap ve epikotil eksplantlarından 1 veya 2 mg L-1 NAA konsantrasyonu + 0.5 mg L-1 kinetin ile sağlanmıştır. En iyi bitki rejenerasyonu değerleri (3.56 ve 3.20 sürgün/kallus) ise epikotil ve hipokotil parçalarının 1 mg L-1 NAA içeren MS ortamında kültüre alınmasıyla elde edilmiştir. Oluşan sürgünler, 1 mg L-1 NAA içeren ½ MS ortamında kolayca köklendirilmiş ve büyük ölçüde dış ortama aktarılmıştır.
To investigate the regeneration potential of Lotus maritimus L., the stem, hypocotyl, cotyledon, root, and epicotyl were cultured in MS media containing different combinations of α-naphthalene acetic acid (NAA) concentrations (1, 2, and 4 mg L-1) and cytokinin types [0 (control), 1 mg L-1 of 6-Benzilaminopurin (BAP), and 0.5 mg L-1 of kinetin]. The highest callus induction was achieved with the stem and epicotyl explants at 1 or 2 mg L-1 concentrations of NAA plus 0.5 mg L-1 of kinetin. The best plant regeneration values (3.56 and 3.20 shoots per callus) were determined with the epicotyl and hypocotyl cultured in MS media containing 1 mg L-1 of NAA. The obtained shoots were easily rooted in half-strength MS media containing 1 mg L-1 NAA and substantially adapted to the external environment.
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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-3865-059X
Author: Pelin GÖKÇE
Institution: Eskişehir Osmangazi Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü
Country: Turkey


Orcid: 0000-0002-4653-5567
Author: Süleyman AVCI (Primary Author)
Institution: Eskişehir Osmangazi Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü
Country: Turkey


Thanks This article is based on Pelin GÖKÇE’s master thesis entitled with "Determination of in vitro regeneration ability of Lotus maritimus L."
Dates

Application Date : December 10, 2019
Acceptance Date : March 17, 2020
Publication Date : August 31, 2020

APA Gökçe, P , Avcı, S . (2020). In Vitro Regeneration Potential of Lotus maritimus L. (Leguminosae) . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (4) , 870-875 . DOI: 10.18016/ksutarimdoga.vi.657729