Keratinase enzyme production was accomplished from Trichophyton sp. Tr-9 in minmal medium containing feather meal. Enzyme was purified with Ammonium sulfate, Sephadeks G-100 and DEAE Sepharose column. Maxiumum keratinolitik activity was obtained at pH 7.5 and 37°C. Enzyme was higly stabil between pH 5.5-7.5 and 20-40oC. With the SDS-Page analysis of the enzyme, molecular weight of the enzyme was calculated as 34 kDa. CaCI2 (5mM) had a stimultory effect (148%) on enzyme. On the other hand, EDTA (5mM) and SDS (%1) inhibited enzyme acitivity up to 49% and 49%, respectively. PMSF (1- 5mM) has strongly inhibited enzyme. As a result, physico-chemical properties of the enzymeshowed that Trichophyton sp. Tr-9 could be useful in various industrial and biotechnological applications.
|Journal Section||RESEARCH ARTICLE|
|Supporting Institution||K.S.Ü. BAP|
|Thanks||Bu çalışma KSU Bilimsel araştırmalar birimi tarafından 2009/4-6M Nolu proje ile desteklenmiştir.|
: January 22, 2020
|APA||Parlak, D , Tanış, H , Aygan, A . (2020). Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (5) , 1135-1143 . DOI: 10.18016/ksutarimdoga.vi.678756|