Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Dönay Parlak; Hüseyin Tanış; Ashabil Aygan

doi:10.18016/ksutarimdoga.vi.678756

EN TR

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Öz

Keratinaz aktivitesi gösteren Trichophyton sp. Tr-9 suşu keratin tozu- feather meal içeren minimal besiyerinde büyütürek enzim üretimi gerçekleştirilmiştir. Süpernatanttan Amonyum sülfat çöktürme sonrası, Sephadeks G-100 ve DEAE Sepharose kolon kromatografi uygulamaları ile enzim saflaştırılması yapılmıştır. Maksimum keratinolitik aktivite pH 7.5 ve 37°C’de gözlenmiştir. Enzim pH 5.5-8.0 ve 20°C-40°C sıcaklık aralıklarında stabilite gösterirken, enziminin moleküler ağırlığı SDS page ve zimogram analizlerinde yaklaşık 34 kDa olarak hesaplandı. CaCI2 (5mM) keratinaz aktivitesini (%148) stimüle edici etki gösterdi. Diğer taraftan EDTA (5mM) ve SDS (%1) sırasıyla %49, % 49 etki göstererek kısmen inhibe etti, PMSF (1mM ve 5mM) ile tam inaktivasyon elde edildi. Sonuç olarak, enzimin fiziko-kimyasal özellikleri birçok endüstriyel ve biyoteknolojik uygulamalarda kullanışlı olabileceğini göstermiştir.

Anahtar Kelimeler

Trichophyton sp.,Keratinaz,Saflaştırma,Karakterizasyon

Destekleyen Kurum

K.S.Ü. BAP

Proje Numarası

2009/6-4M

Teşekkür

Bu çalışma KSU Bilimsel araştırmalar birimi tarafından 2009/4-6M Nolu proje ile desteklenmiştir.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Dönay Parlak
0000-0002-8940-5842
Türkiye

Hüseyin Tanış ^*
0000-0002-2012-7864
Türkiye

Ashabil Aygan
0000-0003-4936-9872
Türkiye

Yayımlanma Tarihi

31 Ekim 2020

Gönderilme Tarihi

22 Ocak 2020

Kabul Tarihi

13 Mart 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 23 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.678756

IZ

https://izlik.org/JA35NA35ZX

Kaynak Göster

RIS / Bibtex

APA

Parlak, D., Tanış, H., & Aygan, A. (2020). Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(5), 1135-1143. https://doi.org/10.18016/ksutarimdoga.vi.678756

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Isolation and Partial Characterizatıon of Keratinase from Trichophyton sp.

Abstract

Keywords

Trichophyton sp. Suşundan Keratinaz Üretimi, Saflaştırılması ve Kısmi Karakterizasyonu

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149