Research Article
Production Profiles of Extended Spectrum Beta Lactamase of Clinic Isolates and Determination of Cell Components with FTIR
Abstract
In this research, the identification of cell components by FT-IR (Fourier Transform Infrared) and ESBL production of Enterobacteriaceae strains isolated from inpatients in Kilis state hospital were studied. Cell components of isolates were detected by using FTIR spectroscopy (4000-400 cm-1). ESBL production of strains was investigated with the cefepime-clavulanic acid, cefotetan-cloxacillin, cefotaxime-clavulanic acid and ceftazidime-clavulanic acid E-strip. According to the E-strip results, it can be stated that only five isolates of Enteric strain were able to produce ESBL. Cefotetan/cefotetan+cloxacillin ratio was determined to be ≥8 µg/mL for Salmonella spp. This ratio was indicated that Salmonella spp. produced C group beta lactamase in Ambler classificataion. Our FTIR analysis revealed that FTIR spectroscopy can be routinely used in the investigation of cell components although it is an unsuccessful method for diagnosis of closely-related species.
References
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- Bauer, AW, Kirby WM; Sherris JC, Turck M 1966. Antibiotic susceptibility testing by a standardized single disk method. American J Clin Pathol 45(4): 493-496.
- Bush K 2001. New beta-lactamases in gram-negative bacteria: Diversity and impact on the selection of antimicrobial therapy. Clin Infect Dis 32:1085-1089.
- Diagbouga S, Salah FD, Sadji AY, Dabire AM, Nadembega C, Kere AB, Soubeiga ST, Ouattar AK, Zohoncon T, Belemgnegre M, Karou S, Simpore J 2016. Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca. J Clin Diagn Res 4(1): 1-7.
- EL-Naghy WS, Wafy AA, Elfar NN, Taha A, Shahba A, Noor-eldeen NM, Nosair NA 2014. Multiplex PCR for detection of bla CTX–M Genes among the extended spectrum beta lactamase (ESBL) producing gram-negative isolates. Egyptian J Med Microbiol 23(3): 107-114.
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- Güzel M, Genç Y, Aksoy A, Moncheva P, Hristova P 2015. Comparison of three different methods for detection of ESBL production and antibiotic resistance percentage of ESBL producing Gram negative bacteria. Türk Hij Den Biyol Derg72(2): 131-138.
- Iroha IR, Okoye E, Osigwe CA, Moses IB, Ejikeugwu CP, Nwakaeze AE 2017. Isolation, Phenotypic Characterization and Prevalence of ESBL-Producing Escherichia Coli and Klebsiella Species from Orthopedic Wounds in National Orthopedic Hospital Enugu (NOHE), South East Nigeria. J PharmCare Health Syst 4(4): 1-5.
- Leylabadlo HE, Pourlak T, Bialvaei AZ, Aghazadeh M, Asgharzadeh M, Kafill HS 2017) Extended-Spectrum Beta-Lactamase Producing Gram Negatıve Bacteria in Iran: A Review. J Infect Dis 11(2): 39-53.
- Lim KT, Yasin R, Yeo CC, Puthucheary S, Thong KL 2009. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. J Biomed Biotechnol, 2009: 1-10.
- Livermore DM 2012. Current Epidemiology and Growing Resistance of Gram-Negative Pathogens. The Korean J Int Med 27(2): 128–142.
- Mura S, Greppi G, Marongiu ML, Roggero PP, Ravindranath SP, Mauer LJ, Schibeci N, Perria F, Piccinini M, Innocenzi P, Irudayaraj J 2012. FTIR nanobiosensors for Escherichia coli detection. Beilstein J Nanotechnol 3: 485-492.
- Naumann D, Helm, D, Labischinski H 1991. Microbiological Characterizations by FT-IR Spectroscopy. Nature. 351: 81-82.
- Oberreuter H, Seiler H, Scherer S 2002. Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy. Int J Syst Evol Microbiol 52: 91-100.
- Puzey KA, Gardner PJ, Petrova VK, Donnelly CW, Petrucci G 2008. Automated species and strain identification of bacteria in complex matrices using FTIR spectroscopy. Int Soc Opt Eng 6954(3): 1-9.
- Ricciardi V, Portaccio M, Piccolella S, Manti L, Pacifico S, Lepore M 2017. Study of SH-SY5Y cancer cell response to treatment with polyphenol extracts using FT-IR spectroscopy. Biosensors 57(7): 1-16.
- Rupp ME, Fey PD 2003. Extended spectrum β-Lactamase (ESBL)-producing Enterobacteriaceae considerations for diagnosis, prevention and drug treatment. Drugs 63(4): 353-365.
- Sharma J, Sharma M, Ray P 2010. Detection of TEM & SHV genes in Escherichia coli & Klebsiella pneumoniae isolates in a tertiary care hospital from India. Indian J Med Res132: 332-336.
- Storberg V 2014. ESBL-producing Enterobacteriaceae in Africa a non-systematic literature review of research published 2008-2012. Infect Ecol Epidemiol 4: 1-16.
- Suntsova AYu, Guliev RR, Popov DA, Vostrikova TYu, Dubodelov DV, Shchegolikhin AN, Laypanov BK, Priputnevich TV, Shevelev AB, Kurochkin IN 2018. Identification of Microorganisms by Fourier-Transform Infrared Spectroscopy. Bulletin of Russian State Med Uni 4: 50-57.
Klinik İzolatların Genişlemiş Spektrumlu Beta Laktamaz Üretimi Profillerinin Belirlenmesi ve Hücre Bileşenlerinin FTIR İle Tespiti
Abstract
Bu çalışmada Kilis Devlet Hastanesinde yatan hastalardan izole edilen Enterobacteriaceae bakterilerin tanımlanması ve izolatların GSBL üretimi profillerinin belirlenmesi çalışılmıştır. İzolatların hücre bileşenleri FTIR (Fourier Transform Infrared) spektroskopisi kullanılarak (4000-400 cm-1) tespit edilmiştir. Suşların GSBL üretim profilleri sefotetan-kloksasilin, sefepim-klavulanik asit, sefotaksim- klavulanik asit ve seftazidim- klavulanik asit E test şeritleri ile incelenmiştir. E-şerit sonuçlarına göre sadece 5 hastane izolatından sadece Enterik olarak tanımlanan suşun GSBL üreticisi olduğu belirlenmiştir. Salmonella spp. için sefotetan/sefotetan+kloksasilin oranın ≥8 µg/mL olması suşun Ambler sınıflandırmasında C grubu (AmpC) beta-laktamaz üreticisi olduğuna işaret etmektedir. FTIR spektroskopisinin bakterilerin hücre bileşenlerinin incelenmesinde rutin olarak kullanılabileceği ancak yakın türlerin tanımlanmasında başarılı bir yöntem olmadığı ortaya konmaktadır.
References
- Başyiğit Kılıç G, Karahan AG 2010. Fourıer dönüşümlü kızılötesi (FTIR) spektroskopisi ve laktik asit bakterilerinin tanısında kullanılması. Gıda 35(6): 445-452.
- Bauer, AW, Kirby WM; Sherris JC, Turck M 1966. Antibiotic susceptibility testing by a standardized single disk method. American J Clin Pathol 45(4): 493-496.
- Bush K 2001. New beta-lactamases in gram-negative bacteria: Diversity and impact on the selection of antimicrobial therapy. Clin Infect Dis 32:1085-1089.
- Diagbouga S, Salah FD, Sadji AY, Dabire AM, Nadembega C, Kere AB, Soubeiga ST, Ouattar AK, Zohoncon T, Belemgnegre M, Karou S, Simpore J 2016. Detection of High Prevalence of TEM/SHV/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca. J Clin Diagn Res 4(1): 1-7.
- EL-Naghy WS, Wafy AA, Elfar NN, Taha A, Shahba A, Noor-eldeen NM, Nosair NA 2014. Multiplex PCR for detection of bla CTX–M Genes among the extended spectrum beta lactamase (ESBL) producing gram-negative isolates. Egyptian J Med Microbiol 23(3): 107-114.
- Garip Ş 2005. The Characterization of Bacteria with Fourier Transform Infrared Spectroscopy. Ortadoğu Teknik Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, 99sy.
- Güzel M, Genç Y, Aksoy A, Moncheva P, Hristova P 2015. Comparison of three different methods for detection of ESBL production and antibiotic resistance percentage of ESBL producing Gram negative bacteria. Türk Hij Den Biyol Derg72(2): 131-138.
- Iroha IR, Okoye E, Osigwe CA, Moses IB, Ejikeugwu CP, Nwakaeze AE 2017. Isolation, Phenotypic Characterization and Prevalence of ESBL-Producing Escherichia Coli and Klebsiella Species from Orthopedic Wounds in National Orthopedic Hospital Enugu (NOHE), South East Nigeria. J PharmCare Health Syst 4(4): 1-5.
- Leylabadlo HE, Pourlak T, Bialvaei AZ, Aghazadeh M, Asgharzadeh M, Kafill HS 2017) Extended-Spectrum Beta-Lactamase Producing Gram Negatıve Bacteria in Iran: A Review. J Infect Dis 11(2): 39-53.
- Lim KT, Yasin R, Yeo CC, Puthucheary S, Thong KL 2009. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. J Biomed Biotechnol, 2009: 1-10.
- Livermore DM 2012. Current Epidemiology and Growing Resistance of Gram-Negative Pathogens. The Korean J Int Med 27(2): 128–142.
- Mura S, Greppi G, Marongiu ML, Roggero PP, Ravindranath SP, Mauer LJ, Schibeci N, Perria F, Piccinini M, Innocenzi P, Irudayaraj J 2012. FTIR nanobiosensors for Escherichia coli detection. Beilstein J Nanotechnol 3: 485-492.
- Naumann D, Helm, D, Labischinski H 1991. Microbiological Characterizations by FT-IR Spectroscopy. Nature. 351: 81-82.
- Oberreuter H, Seiler H, Scherer S 2002. Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy. Int J Syst Evol Microbiol 52: 91-100.
- Puzey KA, Gardner PJ, Petrova VK, Donnelly CW, Petrucci G 2008. Automated species and strain identification of bacteria in complex matrices using FTIR spectroscopy. Int Soc Opt Eng 6954(3): 1-9.
- Ricciardi V, Portaccio M, Piccolella S, Manti L, Pacifico S, Lepore M 2017. Study of SH-SY5Y cancer cell response to treatment with polyphenol extracts using FT-IR spectroscopy. Biosensors 57(7): 1-16.
- Rupp ME, Fey PD 2003. Extended spectrum β-Lactamase (ESBL)-producing Enterobacteriaceae considerations for diagnosis, prevention and drug treatment. Drugs 63(4): 353-365.
- Sharma J, Sharma M, Ray P 2010. Detection of TEM & SHV genes in Escherichia coli & Klebsiella pneumoniae isolates in a tertiary care hospital from India. Indian J Med Res132: 332-336.
- Storberg V 2014. ESBL-producing Enterobacteriaceae in Africa a non-systematic literature review of research published 2008-2012. Infect Ecol Epidemiol 4: 1-16.
- Suntsova AYu, Guliev RR, Popov DA, Vostrikova TYu, Dubodelov DV, Shchegolikhin AN, Laypanov BK, Priputnevich TV, Shevelev AB, Kurochkin IN 2018. Identification of Microorganisms by Fourier-Transform Infrared Spectroscopy. Bulletin of Russian State Med Uni 4: 50-57.
There are 20 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Structural Biology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | October 31, 2020 |
| Submission Date | February 6, 2020 |
| Acceptance Date | April 17, 2020 |
| Published in Issue | Year 2020Volume: 23 Issue: 5 |
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