Year 2020, Volume 23 , Issue 5, Pages 1106 - 1113 2020-10-31

Klinik İzolatların Genişlemiş Spektrumlu Beta Laktamaz Üretimi Profillerinin Belirlenmesi ve Hücre Bileşenlerinin FTIR İle Tespiti
Production Profiles of Extended Spectrum Beta Lactamase of Clinic Isolates and Determination of Cell Components with FTIR

Hatice Aysun MERCİMEK TAKCI [1] , Neslihan ÇEVİK [2] , Fatma Esen SARIGÜLLÜ ÖNALAN [3]


Bu çalışmada Kilis Devlet Hastanesinde yatan hastalardan izole edilen Enterobacteriaceae bakterilerin tanımlanması ve izolatların GSBL üretimi profillerinin belirlenmesi çalışılmıştır. İzolatların hücre bileşenleri FTIR (Fourier Transform Infrared) spektroskopisi kullanılarak (4000-400 cm-1) tespit edilmiştir. Suşların GSBL üretim profilleri sefotetan-kloksasilin, sefepim-klavulanik asit, sefotaksim- klavulanik asit ve seftazidim- klavulanik asit E test şeritleri ile incelenmiştir. E-şerit sonuçlarına göre sadece 5 hastane izolatından sadece Enterik olarak tanımlanan suşun GSBL üreticisi olduğu belirlenmiştir. Salmonella spp. için sefotetan/sefotetan+kloksasilin oranın ≥8 µg/mL olması suşun Ambler sınıflandırmasında C grubu (AmpC) beta-laktamaz üreticisi olduğuna işaret etmektedir. FTIR spektroskopisinin bakterilerin hücre bileşenlerinin incelenmesinde rutin olarak kullanılabileceği ancak yakın türlerin tanımlanmasında başarılı bir yöntem olmadığı ortaya konmaktadır.

In this research, the identification of cell components by FT-IR (Fourier Transform Infrared) and ESBL production of Enterobacteriaceae strains isolated from inpatients in Kilis state hospital were studied. Cell components of isolates were detected by using FTIR spectroscopy (4000-400 cm-1). ESBL production of strains was investigated with the cefepime-clavulanic acid, cefotetan-cloxacillin, cefotaxime-clavulanic acid and ceftazidime-clavulanic acid E-strip. According to the E-strip results, it can be stated that only five isolates of Enteric strain were able to produce ESBL. Cefotetan/cefotetan+cloxacillin ratio was determined to be ≥8 µg/mL for Salmonella spp. This ratio was indicated that Salmonella spp. produced C group beta lactamase in Ambler classificataion. Our FTIR analysis revealed that FTIR spectroscopy can be routinely used in the investigation of cell components although it is an unsuccessful method for diagnosis of closely-related species.

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Primary Language tr
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-5394-4959
Author: Hatice Aysun MERCİMEK TAKCI (Primary Author)
Institution: KİLİS 7 ARALIK ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-9631-8221
Author: Neslihan ÇEVİK
Institution: KİLİS 7 ARALIK ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-1374-4338
Author: Fatma Esen SARIGÜLLÜ ÖNALAN
Institution: KİLİS 7 ARALIK ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : February 6, 2020
Acceptance Date : April 17, 2020
Publication Date : October 31, 2020

APA Merci̇mek Takcı, H , Çevi̇k, N , Sarıgüllü Önalan, F . (2020). Klinik İzolatların Genişlemiş Spektrumlu Beta Laktamaz Üretimi Profillerinin Belirlenmesi ve Hücre Bileşenlerinin FTIR İle Tespiti . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (5) , 1106-1113 . DOI: 10.18016/ksutarimdoga.vi.685696