Abstract
Çalışmada 5 cinse (Cephalanthera Rich., Dactylorhiza Neck. ex Nevski, Epipactis Zinn, Serapias L., Platanthera Rich.) ait 6 taksonda labellum mikromorfolojisi Taramalı Elektron mikroskobu (SEM) yöntemi ile incelenmiş ve sonuçlar karşılaştırmalı tablolar halinde sunulmuştur. Örnekler, araziden toplandığı sırada % 70 alkole alınmış, bir kısmı da herbaryum kurallarına göre kurutularak teşhisleri yapılmıştır. Alkolden çıkarılan labellum örnekleri, bisturi ve iğnelerle stereomikroskop altında dissekte edilerek doğrudan üzeri çift taraflı yapışkan bantlar bulunan ve SEM cihazına ait çelik aparatlara yerleştirilmiştir. Bir gece kadar bekletilen örnekler, doğrudan (SEM) ile incelenmiş ve labellum mikrofotoğrafları çekil-miştir. Bulgulara göre; Platanthera chlorantha labellumunun tüm bazal, lateral ve medyan bölgelerinde düzensiz hücreli, yüzeyi mikro rugulate ve yoğun ruminate şeklinde bir morfolojiye sahip olduğu ve bu özellikleri ile diğer tüm incelenen taksonlardan oldukça farklı olduğu tespit edilmiştir. Konikal papilla oluşumları, Cephalanthera epipactoides ve Dactylorhiza romana’nın farklı labellum bölgelerinde bulunmuştur. Epikil ve Hipokil özellikli Epipactis ve Serapias taksonları ise birbirlerinden epidermal hücre şekli, yanal çeper özellikleri ve yüzey özellikleri ile farklılık göstermiştir. Sonuçta labellum ayrıntılı yüzey morfolojisi ve mahmuz özelliklerinin, taksonların ayırımında faydalı olduğu görülmüştür.
Keywords
Supporting Institution
Trakya Üniversitesi
Project Number
TÜBAP 2020 /94
Thanks
Bu çalışma, Trakya Üniversitesi TÜBAP 2020 /94 kodlu proje ile desteklenmiştir.
References
- Ascensao L, Francisco A, Cotrim H and Pais MS 2005. Comparative structure of the labellum in Ophrys fusca and O. lutea (Orchidaceae). American Journal of Botany (7): 1059–1067.
- Bell AK, Roberts DL, Hawkins JA, Rudall PJ, Box MS and Bateman RM 2009. Comparative micromorphology of nectariferous and nectarless labellar spurs in selected clades of subtribe Orchidinae (Orchidaceae). Botanical Journal of the Linnean Society 160: 369–387.
- Box MS, Bateman RM, Glover BJ, Rudall PJ 2008. Floral ontogenetic evidence of repeated speciation via paedomorphosis in subtribe Orchidinae (Orchidaceae). Botanical Journal of the Linnean Society Soc 157: 429–454.
- Bradshaw E, Rudall PJ, Devey DS, Thomas MM, Glover BJ and Bateman RM 2010. Comparative labellum micromorphology of the sexually deceptive temperate orchid genus Ophrys: diverse epidermal cell types and multiple origins of structural colour. Botanical Journal of the Linnean Society 162: 504–540.
- Davies KL and Turner MP 2004. Morphology of floral papillae in Maxillaria Ruiz. & Pav. (Orchidaceae). Ann of Bot 93: 75-86.
- Delforge P 2005. Guide des orchidees d’Europe, d’Afrique du Nord et du Proche Orient. Delachaux et Niestle, Paris, pp 82-95
- Lumaga MRB, Pellegrino G, Bellusci F, Perrotta E, Perrotta I, Musacchio A 2012. Comparative floral micromorphology in four sympatric species of Serapias (Orchidaceae). Botanical Journal of the Linnean Society 169: 714-724.
- Rossi W, Corrias B, Ardurino P, Cianchi R, Bullini L 1992. Gene variation and gene flow in Orchis morio (Orchidaceae) from Italy. Plant Systematics and Evolution 179: 43-58.
- Rudall PJ, Bateman RM 2002. Roles of synorganization, zygomorphy and heterotopy in floral evolution: the gynostemium and labellum of orchids and other lilioid monocots. Biological Reviews 77: 403–441.
- Sezik E 1984. Orkidelerimiz, Türkiye’nin Orkideleri. Sandoz Kültür Yayınları, no. 6, İstanbul.
- Stpiczynska M 2003. Nectar resorption in the spur of Platanthera chlorantha (Custer) Rchb. Orchidaceae: structural and microautoradiographic study. Plant Systematics and Evolution 238: 119–126.
- Süngü Şeker Ş, Akbulut MK, Şenel G 2016. Labellum micromorphology of some orchid genera (Orchidaceae) distributed in the Black Sea region in Turkey. Turkish Journal of Botany 40: 1-14.
- Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA 1968. Flora Europeae, Vol. 5, Alismataceae to Orchidaceae (Monocotyledones). Cambridge University Press, Cambridge. pp. 344-348.
Abstract
In this study, micromorphology of labellum in 6 taxa belonging to 5 genera (Cephalanthera Rich., Dactylorhiza Neck. ex Nevski, Epipactis Zinn, Serapias L., Platanthera Rich.) was examined by Scanning Electron Microscopy (SEM) and the results were presented in comparative tables. While the samples were collected from the field, they were taken to 70% alcohol, some of them were dried according to the herbarium rules and diagnosed. The labellum samples extracted from alcohol were dissected under the stereomicroscope with scalpel and needles and placed directly into the steel apparatus of the SEM with double-sided adhesive tapes. The samples, from which alcohol was completely evaporated by waiting for one night, were examined directly with Scanning Electron Microscope (SEM) and labellum microphotographs were taken. According to the findings; It has been determined that Platanthera chlorantha labellum has an irregular cell, micro rugulate surface and dense ruminate morphlogy in all basal, lateral and median regions, and it is quite different from all other examined taxa with these features. Conical papillae formations have been found in different labellum regions of Cephalanthera epipactoides and Dactylorhiza romana. Epipactis and Serapias taxa with epicile and hypocile featured differ from each other with their epidermal cell shape, lateral wall features and surface features. As a result, labellum detailed surface morphlogy and spur features were found to be useful in distinguishing taxa.
Keywords
Project Number
TÜBAP 2020 /94
References
- Ascensao L, Francisco A, Cotrim H and Pais MS 2005. Comparative structure of the labellum in Ophrys fusca and O. lutea (Orchidaceae). American Journal of Botany (7): 1059–1067.
- Bell AK, Roberts DL, Hawkins JA, Rudall PJ, Box MS and Bateman RM 2009. Comparative micromorphology of nectariferous and nectarless labellar spurs in selected clades of subtribe Orchidinae (Orchidaceae). Botanical Journal of the Linnean Society 160: 369–387.
- Box MS, Bateman RM, Glover BJ, Rudall PJ 2008. Floral ontogenetic evidence of repeated speciation via paedomorphosis in subtribe Orchidinae (Orchidaceae). Botanical Journal of the Linnean Society Soc 157: 429–454.
- Bradshaw E, Rudall PJ, Devey DS, Thomas MM, Glover BJ and Bateman RM 2010. Comparative labellum micromorphology of the sexually deceptive temperate orchid genus Ophrys: diverse epidermal cell types and multiple origins of structural colour. Botanical Journal of the Linnean Society 162: 504–540.
- Davies KL and Turner MP 2004. Morphology of floral papillae in Maxillaria Ruiz. & Pav. (Orchidaceae). Ann of Bot 93: 75-86.
- Delforge P 2005. Guide des orchidees d’Europe, d’Afrique du Nord et du Proche Orient. Delachaux et Niestle, Paris, pp 82-95
- Lumaga MRB, Pellegrino G, Bellusci F, Perrotta E, Perrotta I, Musacchio A 2012. Comparative floral micromorphology in four sympatric species of Serapias (Orchidaceae). Botanical Journal of the Linnean Society 169: 714-724.
- Rossi W, Corrias B, Ardurino P, Cianchi R, Bullini L 1992. Gene variation and gene flow in Orchis morio (Orchidaceae) from Italy. Plant Systematics and Evolution 179: 43-58.
- Rudall PJ, Bateman RM 2002. Roles of synorganization, zygomorphy and heterotopy in floral evolution: the gynostemium and labellum of orchids and other lilioid monocots. Biological Reviews 77: 403–441.
- Sezik E 1984. Orkidelerimiz, Türkiye’nin Orkideleri. Sandoz Kültür Yayınları, no. 6, İstanbul.
- Stpiczynska M 2003. Nectar resorption in the spur of Platanthera chlorantha (Custer) Rchb. Orchidaceae: structural and microautoradiographic study. Plant Systematics and Evolution 238: 119–126.
- Süngü Şeker Ş, Akbulut MK, Şenel G 2016. Labellum micromorphology of some orchid genera (Orchidaceae) distributed in the Black Sea region in Turkey. Turkish Journal of Botany 40: 1-14.
- Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA 1968. Flora Europeae, Vol. 5, Alismataceae to Orchidaceae (Monocotyledones). Cambridge University Press, Cambridge. pp. 344-348.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Structural Biology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | TÜBAP 2020 /94 |
| Publication Date | February 28, 2022 |
| Submission Date | February 18, 2021 |
| Acceptance Date | April 27, 2021 |
| Published in Issue | Year 2022Volume: 25 Issue: 1 |
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