Van Gölü Havzası’ndan Toplanan Bazı Kavun Genotiplerinde Virüse Karşı Dayanıklılığın Mekanik İnokulasyon ve Moleküler Yöntemlerle Belirlenmesi
Abstract
Kavun (Cucumis melo L.) Türkiye ‘nin farklı coğrafik bölgelerinde yetiştirilen önemli bir sebze türüdür. Zucchini yellow mosaic virüs (ZYMV) başta olmak üzere cucumber mosaic virüs (CMV) ve watermelon mosaic virüs (WMV) kavunda oluşturduğu yıkıcı hasarlardan dolayı büyük ekonomik kayıplara neden olmaktadır. Bu çalışmada, Van Gölü Havzası’ndan toplanmış olan 22 farklı kavun genotipi ile birlikte 3 dayanıklı genotip ve 2 ticari çeşit bu üç virüse karşı mekanik inokülasyon ve moleküler yöntemlerle taranmıştır. Çalışmada ilk etapta ZYMV virüsü kullanılarak kavun genotiplerine virüs inokulasyonu gerçekleştirilmiş ve genotiplerde morfolojik gözlemler yapılarak 0-5 skalasına göre hastalık şiddeti belirlenmiştir. Çalışmanın ikinci aşamasında bu genotiplerde 3 adet SCAR ve 4 adet CAPS olmak üzere 7 adet primer ile tarama yapılarak ZYMV, CMV ve WMV’ ye karşı dayanıklılık ile ilgili belirteçlerin varlığı tespit edilmeye çalışılmıştır. Şiddetli ZYMV izolatına karşı mekanik olarak test edilen genotiplerin hiçbirinin virüse tolerant, dayanıklı ya da immun olmadığı saptanmıştır. Kavun genotiplerinde görülen hastalık şiddetinin %8.80-95.00 arasında değiştiği belirlenmiştir. SCOPE14541 primerinde 21 genotipte bant tespit edilirken, Creb-2 dayanıklılık genine bağlı olarak geliştirilen SCAPB051046 primerinde 25 genotipten yalnızca U6, U13 ve YYU6 genotiplerinde bant varlığı belirlenmiştir. ZYMV ve WMV’ ye karşı dayanıklılık genlerine bağlı olarak geliştirilmiş olan VirSq-F19 primeri YYU47 genotipi dışında tüm genotiplerde bant vermiştir. Zym dayanıklılık geni ile ilişkili 4 CAPS primerinden sadece ikisinden sonuç elde edilmiştir.
Keywords
Supporting Institution
Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırmalar Proje Koordinatörlüğü, Öğretim Üyesi Yerleştirme Programı (ÖYP)
Project Number
FYL-2017-5899
Thanks
Virüs kültürünün temininde yardimlarindan dolayı Dr. Öğr. Üyesi Mustafa USTA’YA teşekkür ederiz.
References
- Amano M, Mochizuki A, Kawagoe Y, Iwahori K, Niwa K, Svoboda J, Maeda T, Imura Y 2013. High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber. Theoretical and Applied Genetics, 126: 2983–2993.
- Cullings KW 1992. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology, 1: 233-240.
- Daryono BS, Wakui K, Natsuaki KT 2009. Development of random amplified polymorphism DNA markers linked to CMV-B2 resistance gene in melon. Hayati Journal of Biosciences, 35: 142-146.
- Doyle JJ, Dickson EE 1987. Preservation of plant samples for DNA restriction endonuclease analysis. Taxon, 36: 715-722.
- Doyle JJ, Doyle JL 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19: 11-15.
- Ekbiç E, Fidan H, Yıldız M, Abak K 2010. Screening of Turkish melon accessions for resistance to ZYMV, WMV and CMV. Notulae Scientia Biologicae, 2 (1): 55-57.
- Ekincialp A 2019. The differences among melon genotypes and varieties under salt stress based on certain morphological and physiological properties-mixture modeling and principal component analysis (PCA). Applied Ecology and Environmental Research, 17: 2965-2981.
- Erdinç Ç 2018. Changes in ion (K, Ca and Na) regulation, antioxidant enzyme activity and photosynthetic pigment content in melon genotypes subjected to salt stress - A mixture modeling analysis. Acta Scientiarum Polonorum-Hortorum Cultus, 17: 165-183.
- Erdinç Ç Ekincialp A, Yıldız M, Kabay T, Türkmen Ö, Şensoy S. 2013. Molecular genetic diversity in Lake Van Basin melons (Cucumis melo L.) based on RAPD and ISSR markers. Yuzuncu Yil University Journal of Agricultural Science, 23(3): 264- 270.
- Gallitelli D 2000. The ecology of Cucumber mosaic virus and sustainable agriculture. Virus Research, 71:9-21.
- Kabelka E, Grumet R 1997. Inheritance of resistance to the Moroccan watermelon mosaic virus in the cucumber line TMG-1 and co-segregation with zucchini yellow mosaic virus resistance. Euphytica, 95: 237– 242.
- Kim DK, Seo S, Kwon SB, Park Y 2016. Development of RAPD and SCAR markers related to watermelon mosaic virus and zucchini yellow mosaic virus resistance in Cucurbita moschata. Hortic. Environ. Biotechnol., 57(1): 61-68. Doi: 10.1007/s13580-016 0090-0.
- Kurtar ES, Seymen M, Kal Ü 2020. An overview of doubled haploid plant production in Cucurbita species. Yuzuncu Yil University Journal of Agricultural Science, 30 (3): 510-520.
- Ling K, Harris KR, Meyer JDF, Levi A, Güner N, Wehner TC, Bendahmane A, Havey MJ. 2009. Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to zucchini yellow mosaic virus. Theor. Appl. Genet., 120(1): 191-200. doi: 10.1007/s00122-009-1169-0.
- Pitrat M, Lecoq H 1984. Inheritance of zucchini yellow mosaic virus resistance in Cucumis melo L. Euphytica, 33: 57-61.
- Sensoy S, Demir S, Turkmen O, Erdinc C, Durak Demirer E 2012. Variation in the reaction of Lake Van Basin melon genotypes to Fusarium oxysporum f. sp. melonis. Int. J. Agric. Biol., 14: 1024–1026.
- Sensoy S, Sahin U 2012. Genetic Relationships Among Various Sihke Melon Landraces. Yuzuncu Yil University Journal of Agricultural Science, 22(3): 147-154.
- Şensoy S, Turkmen O, Kabay T, Erdinc C, Turan M, Yildiz M 2005. Determination of salinity tolerance of melon genotypes collected from Lake Van Basin. J. Bio-Sci., 5: 637–642.
- Sharifi M, Massumi H, Heydarnejad J, Pour AH, Shaabanian M, Rahimian H 2008. Analysis of the biological and molecular variability of watermelon mosaic virus isolates from Iran. Virus Genes, 37: 304-313.
- Simmons HE, Holmes EC, Gildow FE, Bothe-Goralczyk MA, Stephenson AG 2011. Experimental verification of seed transmission of Zucchini yellow mosaic virus. Plant Diseases, 95(6): 751-754.
- Sipahioğlu HM, Türkmen Ö, Usta M, Güller A, Seymen M, Paksoy M, Fidan S 2015. Bazı yerli çerezlik kabak çeşit adaylarının zucchini yellow mosaic virusüne karşı dayanıklılığının araştırılması. Selçuk Tarım Bilimleri Dergisi, 2 (2): 136-143.
- Townsend GK, Heuberger JW 1943. Methods for estimating losses caused by diseases in fungicide experimentsç. Plant Disease Report, 27: 340-343.
- Türkmen Ö, Şensoy S, Erdinç Ç 2008. Van Gölü Havzası’ ndan toplanan bazı kavun genotiplerinin verim ve verim özelliklerinin belirlenmesi. Selçuk Üniversitesi, Ziraat Fakültesi Dergisi, 22(44): 64-70.
- Yıldız M, Akgül N, Şensoy S 2014. Morphological and molecular characterization of Turkish landraces of Cucumis melo L. Not. Bot. Horti. Agrobo, 42(1): 51-58.
- Yılmaz MA, Lecoq H, Abak K, Baloğlu S, Sarı N 1992. Türkiye’de kabakgil sebze türlerinde zarar yapan virüsler. Türkiye I. Ulusal Bahçe Bitkileri Kongresi, Cilt:II. E.Ü.Ziraat Fakültesi Bornova, İzmir, 13-16 Ekim 1992, 439-442.
Determination of Virus Resistance in Some Melon Accession Collected from Van Lake Basin by Mechanic Inoculation and Molecular Methods
Abstract
Melon (Cucumis melo L.) is an important vegetable crop grown in different geographical regions of Turkey. Zucchini yellow mosaic virus (ZYMV), cucumber mosaic virus (CMV) and watermelon mosaic virus (WMV) cause major economic losses because of their destructive effects on melon. In this study, 22 different melon genotypes collected from the Lake Van Basin, three resistant genotypes and two commercial varieties were screened against these three viruses by mechanical inoculation and molecular methods. In the first step, virus inoculation was performed on melon genotypes using ZYMV virus, and disease severity was determined according to 0-5 scale by performing morphological observations in the genotypes. In second stage of the study the presence of markers related to resistance genes against to ZYMV, CMV and WMV were determined by screening with 7 primers including 3 SCAR primers and 4 CAPS primers in these genotypes. None of the genotypes tested mechanically against the severe ZYMV isolate were found to be tolerant, resistant or immune to the virus. It was determined that the disease severity was ranged between 8.80-95.00% in melon genotypes. While bands were obtained from SCOPE14541 primer in 21 genotypes, only U6, U13 and YYU6 genotypes from 25 genotypes gave bands with SCAPB051046 primer that was developed based on the Creb-2 resistance gene. VirSq-F19 primer that was developed based on the resistance genes to ZYMV and WMV gave bands in all genotypes excluding YYU47. Only two of the 4 CAPS primers associated with the Zym resistance gene produced band.
Keywords
Project Number
FYL-2017-5899
References
- Amano M, Mochizuki A, Kawagoe Y, Iwahori K, Niwa K, Svoboda J, Maeda T, Imura Y 2013. High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber. Theoretical and Applied Genetics, 126: 2983–2993.
- Cullings KW 1992. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology, 1: 233-240.
- Daryono BS, Wakui K, Natsuaki KT 2009. Development of random amplified polymorphism DNA markers linked to CMV-B2 resistance gene in melon. Hayati Journal of Biosciences, 35: 142-146.
- Doyle JJ, Dickson EE 1987. Preservation of plant samples for DNA restriction endonuclease analysis. Taxon, 36: 715-722.
- Doyle JJ, Doyle JL 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19: 11-15.
- Ekbiç E, Fidan H, Yıldız M, Abak K 2010. Screening of Turkish melon accessions for resistance to ZYMV, WMV and CMV. Notulae Scientia Biologicae, 2 (1): 55-57.
- Ekincialp A 2019. The differences among melon genotypes and varieties under salt stress based on certain morphological and physiological properties-mixture modeling and principal component analysis (PCA). Applied Ecology and Environmental Research, 17: 2965-2981.
- Erdinç Ç 2018. Changes in ion (K, Ca and Na) regulation, antioxidant enzyme activity and photosynthetic pigment content in melon genotypes subjected to salt stress - A mixture modeling analysis. Acta Scientiarum Polonorum-Hortorum Cultus, 17: 165-183.
- Erdinç Ç Ekincialp A, Yıldız M, Kabay T, Türkmen Ö, Şensoy S. 2013. Molecular genetic diversity in Lake Van Basin melons (Cucumis melo L.) based on RAPD and ISSR markers. Yuzuncu Yil University Journal of Agricultural Science, 23(3): 264- 270.
- Gallitelli D 2000. The ecology of Cucumber mosaic virus and sustainable agriculture. Virus Research, 71:9-21.
- Kabelka E, Grumet R 1997. Inheritance of resistance to the Moroccan watermelon mosaic virus in the cucumber line TMG-1 and co-segregation with zucchini yellow mosaic virus resistance. Euphytica, 95: 237– 242.
- Kim DK, Seo S, Kwon SB, Park Y 2016. Development of RAPD and SCAR markers related to watermelon mosaic virus and zucchini yellow mosaic virus resistance in Cucurbita moschata. Hortic. Environ. Biotechnol., 57(1): 61-68. Doi: 10.1007/s13580-016 0090-0.
- Kurtar ES, Seymen M, Kal Ü 2020. An overview of doubled haploid plant production in Cucurbita species. Yuzuncu Yil University Journal of Agricultural Science, 30 (3): 510-520.
- Ling K, Harris KR, Meyer JDF, Levi A, Güner N, Wehner TC, Bendahmane A, Havey MJ. 2009. Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to zucchini yellow mosaic virus. Theor. Appl. Genet., 120(1): 191-200. doi: 10.1007/s00122-009-1169-0.
- Pitrat M, Lecoq H 1984. Inheritance of zucchini yellow mosaic virus resistance in Cucumis melo L. Euphytica, 33: 57-61.
- Sensoy S, Demir S, Turkmen O, Erdinc C, Durak Demirer E 2012. Variation in the reaction of Lake Van Basin melon genotypes to Fusarium oxysporum f. sp. melonis. Int. J. Agric. Biol., 14: 1024–1026.
- Sensoy S, Sahin U 2012. Genetic Relationships Among Various Sihke Melon Landraces. Yuzuncu Yil University Journal of Agricultural Science, 22(3): 147-154.
- Şensoy S, Turkmen O, Kabay T, Erdinc C, Turan M, Yildiz M 2005. Determination of salinity tolerance of melon genotypes collected from Lake Van Basin. J. Bio-Sci., 5: 637–642.
- Sharifi M, Massumi H, Heydarnejad J, Pour AH, Shaabanian M, Rahimian H 2008. Analysis of the biological and molecular variability of watermelon mosaic virus isolates from Iran. Virus Genes, 37: 304-313.
- Simmons HE, Holmes EC, Gildow FE, Bothe-Goralczyk MA, Stephenson AG 2011. Experimental verification of seed transmission of Zucchini yellow mosaic virus. Plant Diseases, 95(6): 751-754.
- Sipahioğlu HM, Türkmen Ö, Usta M, Güller A, Seymen M, Paksoy M, Fidan S 2015. Bazı yerli çerezlik kabak çeşit adaylarının zucchini yellow mosaic virusüne karşı dayanıklılığının araştırılması. Selçuk Tarım Bilimleri Dergisi, 2 (2): 136-143.
- Townsend GK, Heuberger JW 1943. Methods for estimating losses caused by diseases in fungicide experimentsç. Plant Disease Report, 27: 340-343.
- Türkmen Ö, Şensoy S, Erdinç Ç 2008. Van Gölü Havzası’ ndan toplanan bazı kavun genotiplerinin verim ve verim özelliklerinin belirlenmesi. Selçuk Üniversitesi, Ziraat Fakültesi Dergisi, 22(44): 64-70.
- Yıldız M, Akgül N, Şensoy S 2014. Morphological and molecular characterization of Turkish landraces of Cucumis melo L. Not. Bot. Horti. Agrobo, 42(1): 51-58.
- Yılmaz MA, Lecoq H, Abak K, Baloğlu S, Sarı N 1992. Türkiye’de kabakgil sebze türlerinde zarar yapan virüsler. Türkiye I. Ulusal Bahçe Bitkileri Kongresi, Cilt:II. E.Ü.Ziraat Fakültesi Bornova, İzmir, 13-16 Ekim 1992, 439-442.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | FYL-2017-5899 |
| Publication Date | August 31, 2022 |
| Submission Date | November 16, 2020 |
| Acceptance Date | September 20, 2021 |
| Published in Issue | Year 2022Volume: 25 Issue: 4 |
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