Karakılçık (KK) is a landrace, of which both bread and durum wheat forms exist. This is the first study that reports mutation induction and detection with agronomic traits and allele specific markers on KK durum wheat landrace. In the study, KK landrace was induced with chemical mutation using sodium azide (NaN3) (3 mM) mutagen to improve agronomic traits. In the research, KK genotype and the 13 M4 individuals (KK-1 to KK-13) were used as plant materials. According to the results, the shortest mutant genotype was KK-10 (125.80 cm), with the highest protein ratio (18.50%) and wet gluten ratio (37.10%), while KK-9 genotype had the highest grain yield (4285.6 kg ha-1). The average polymorphism information content (PIC) was calculated as 0.97, while the average allele number was 15.2 per marker. Glu-B1 (Bx7OE primer) allele was determined on KK-11 and KK-13 genotypes. Wx-A1 allele was found on KK-1, KK-2, KK-4, KK-5 and KK-7 genotypes. Sr49 allele (Sun209) was determined on KK-5 genotype, while Yr45 allele (Xwgp118) was detected on KK-10 genotype. In addition, the high protein content allele Gpc-B1 (UHW89 primer) was found on KK-1, KK-2, KK-3 and KK-4 genotypes. Based on the principal component biplot analysis, it was determined that there was a positive relationship between grain yield (GY) with test weight (TW), grain number and weight per spike (GNS and GWS), and negative relationship with the other traits.
Karakılçık (KK), hem ekmeklik hem de makarnalık buğday formları bulunan yerel bir çeşittir. Bu, yerel KK makarnalık buğday çeşidinde agronomik özellikler ve allel spesifik markörler ile mutasyon tespitini bildiren ilk çalışmadır. Çalışmada, agronomik özellikleri iyileştirmek için sodyum azid (NaN3) (3 mM) mutajeni kullanılarak kimyasal mutasyon ile yerel KK çeşidi muamele edilmiştir. Araştırmada bitki materyali olarak KK genotipi ve 13 M4 bireyi (KK-1 ila KK-13) kullanılmıştır. Elde edilen sonuçlara göre, en yüksek protein oranı (%18.50), yaş gluten oranı (%37.10) ile en kısa mutant genotip KK-10 (125.80 cm) olurken, KK-9 en yüksek tane verimine (4285.6 kg ha-1) sahip genotip olmuştur. Ortalama polimorfizm bilgi içeriği (PIC) 0.97, ortalama allel sayısı markör başına 15.2 olarak hesaplanmıştır. Glu-B1 (Bx7OE) alleli, KK-11 ve KK-13 genotiplerinde, Wx-A1 alleli ise KK-1, KK-2, KK-4, KK-5 ve KK-7 genotiplerinde belirlenmiştir. KK-5 genotipinde Sr49 alleli (Sun209), KK-10 genotipinde ise Yr45 alleli (Xwgp118) tespit edilmiştir. Ayrıca KK-1, KK-2, KK-3 ve KK-4 genotiplerinde yüksek protein içerikli allel olan Gpc-B1 alleli (UHW89) bulunmuştur. Temel bileşen biplot analizine göre, tane verimi (TV) ile hektolitre ağırlığı (HL), başakta tane sayısı ve başakta tane ağırlığı (BTS ve BTA) arasında pozitif, diğer özelliklerle negatif ilişki olduğu belirlenmiştir.
Primary Language | English |
---|---|
Subjects | Agricultural, Veterinary and Food Sciences |
Journal Section | RESEARCH ARTICLE |
Authors | |
Early Pub Date | May 15, 2023 |
Publication Date | August 31, 2023 |
Submission Date | May 23, 2022 |
Acceptance Date | February 27, 2023 |
Published in Issue | Year 2023Volume: 26 Issue: 4 |
International Peer Reviewed Journal
Free submission and publication
Published 6 times a year
KSU Journal of Agriculture and Nature
e-ISSN: 2619-9149