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Molecular Detection of Infectious Viruses in Potato Tubers in Potato Production Areas in Tokat Province

Year 2024, Volume: 27 Issue: 5, 1061 - 1068

Afide Merve Engür Şerife Topkaya

https://doi.org/10.18016/ksutarimdoga.vi.1435134

Abstract

Ninety-one potato tuber samples showing virus symptoms were collected from potato production areas in Niksar, Erbaa, and Artova districts, where potato cultivation is done in Tokat province. Tuber samples were subjected to RT-PCR tests to determine the presence of potato virus Y (PVY), potato virus S (PVS), potato virus X (PVX), and potato leafroll virus (PLRV) viruses. Single or mixed virus infections were detected in 68 (74.72%) of the tuber samples tested. Of the 91 germinated tubers, 67 were infected with PVY (73.62%), 6 were infected with PVS (6.59%), and one was infected with PLRV (1.09%). In addition, PVY+PVS mixed infection was detected in 5 of the tubers infected with PVS, while PVY infection was detected in the tubers infected with PLRV. It was determined that the most common virus in tuber samples collected from different districts of Tokat province was PVY, followed by PVS. Infected tubers used as seeds cause serious losses in yield from year to year. The use of virus-free and certified seeds is the most important factor in preventing viral-related losses. otato leafroll virus (PLRV) viruses. Single or mixed virus infections were detected in 68 (74.72%) of the tuber samples tested.

Keywords

Potato tuber PLRV PVS PVX PVY RT-PCR

Project Number

2018/50

References

  • Arlı-Sökmen, M., Ayan, A.K., & Şevik, M.A. (2005). Trabzon ve Bayburt illerinde tohumluk patates (Solanum tuberosum L.) yumrularında belirlenen virüsler. OMÜ Ziraat Fakültesi Dergisi, 20 (3), 23–26. https://dergipark.org.tr/tr/download/article-file/187576
  • Astruc, N., Marcos, J.F., Macquaire, G., Candresse, T. & Pallas, V. (1996). Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of nucleic acid extraction procedure based on non-organic solvents. European Journal of Plant Pathology, 102, 837–846. https://doi.org/10.1007/BF01877053
  • Bostan, H. & Haliloğlu, K. (2004). Distribution of PLRV, PVS, PVX, and PVY (PVYN, PVY0 and PVYC) in the seed potato tubers in Turkey. Pakistan Journal of Biological Sciences, 7(7), 1140-1143. https://doi: 10.3923/pjbs.2004.1140.1143
  • Bostan, H., Guclu, C., Ozturk, E., I, Ozdemir & Ilbagi, H. (2006). Influence of aphids on the epidemiology of potato virus diseases (PVY, PVS and PLRV) in the high altitude areas of Turkey. Pakistan Journal of Biological Sciences, 9, 759-765. https://doi: 10.3923/pjbs.2006.759.765
  • Bostan, H & Peker, P. K. (2009). The feasibility of tetraplex RT-PCR in the determination of PVS, PLRV, PVX and PVY from dormant potato tubers. African Journal of Biotechnology, 8 (17), 4043-4047 Doi:10.4314/AJB.V8I17.62127
  • Çalı, S. & Yalçın, N. (1991). İthal edilmiş tohumluk patateslerde önemli virüs hastalıklarının DAS-ELISA ve diğer yöntemlerle araştırılması (Bildiriler). VI. Türkiye Fitopatoloji Kongresi, İzmir, Türkiye, 7-11 Ekim 1991, ss. 333–336.
  • Çıtır, A. (1982). Erzurum ve çevresinde tohumluk patateslerdeki virus hastalıkları ve bunların tanılanması üzerinde bazı araştırmalar. Doğa Bilim Dergisi, 6(3), 99-109.
  • Devaux, A., Kromann, P. & Ortiz, O. (2014). Potatoes for sustainable global food security. Potato Research, 57, 185–199. DOI 10.1007/s11540-014-9265-1
  • Duan, G., Zhan, F., Du, Z., Ho, S.Y.W. & Gao, F. (2018). Europe was a hub for the global spread of potato virus S in the 19th century. Virology, 525, 200–204. https://doi.org/10.1016/j.virol.2018.09.022
  • Gümüş, M. & Erkan, S. (1998). Ayvalık ve Altınova yörelerinde üretilen patates çeşitlerinin yumrularında bulunan virüslerin belirlenmesi üzerinde araştırmalar (Bildiriler). VIII. Türkiye Fitopatoloji Kongresi, Ankara, Türkiye, 21-25 Eylül 1998, ss. 348–350.
  • Güner, Ü. & Yorgancı, Ü. (2006). Niğde ve Nevşehir İlleri patates ekiliş alanlarında saptanan viral etmenler. Bitki Koruma Bülteni, 46 (1-4), 35-49. https://dergipark.org.tr/tr/download/article-file/ 41538
  • Jones, E.D. (1988). A current assessment of in vitro culture and other rapid multiplication methods in North America and Europe. Am. Potato J., 65, 209-220. https://doi.org/10.1007/BF02854453
  • Kadakoğlu, B., & Karlı, B., (2022). Afyonkarahisar İlinde Patates Üretiminin Ekonomik Analizi. KSÜ Tarım ve Doğa Dergisi 25 (3), 581-588. https://doi.org/10.18016/ksutarimdoga.vi.947387.
  • Kökten, M. (2007). Ödemiş bölgesinde üretimi yapılan patates yumrularında PVY, PVX, PVS ve PLRV virüslerinin RT-PCR yöntemiyle saptanması (Tez no 200661). [Yüksek lisans tezi. Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Ana Bilim Dalı]. Aydın. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Kreuze, J.F., Souza-Dias, J.A.C., Jeevalatha, A., Figueira, A.R., Valkonen, J.P.T. & Jones, R.A.C. (2020). Viral diseases in Potato. Pages 389-430 in: In The Potato Crop; Campos, H., Ortiz, O., (Eds), Springer, Cham, Switzerland. https://doi.org/ 10.1007/978-3-030-28683-5_11.
  • Lesley, T. & Michael, E.T. (2020). Potato virus Y emergence and evolution from the andes of south america to become a major destructive pathogen of potato and other solanaceous crops worldwide. Viruses, 12, 1–14. https://doi.org/10.3390/ v12121430
  • Mao, Y., Sun, X., Shen, J., Gao, F., Qiu, G., Wang, T., Nie, X., Zhang, W., Gao, Y., & Bai, Y. (2019). Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene. Frontiers in microbiology, 10, 1708. https://doi.org/10.3389/ fmicb.2019.01708
  • Slack, S.A. (1995). Potato viruses with some implications for production and processing in the United States: a history of problems and solutions. Summa Phytopathologica, 21, 273-275.
  • Spiegel, S. & Martin, R.P. (1993). Improved detection of potato leafroll virus in dormant potato tubers and micro tubers by the polymerase chain reaction and ELISA. Annals of Applied Biology, 121, 493-500. https://doi.org/10.1111/j.1744-7348.1993. tb04052.x
  • Topkaya, S. (2020). Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province. Journal of Agricultural Faculty of Gaziosmanpasa University, 37 (1), 53-59. doi:10.13002/jafag4685.
  • Topkaya, Ş., Çelik, A., Santosa, A.I. & Jones, R.A.C. (2023). Molecular Analysis of the Global Population of Potato Virus S Redefines Its Phylogeny, and Has Crop Biosecurity Implications.Viruses, 15, 1104. https://doi.org/ 10.3390/v15051104
  • Zhang, W., Bai, Y.Q., Gao, Y.L., Sheng, Y., Fan, G.Q. & Gen, H.W. (2010). A survey on occurrence frequencies of potato viruses in major potato producing provinces in China. Heilongjiang Agric. Sci., 4, 71–73. https://doi.org/10.1016/j.matlet. 2009.10.009

Tokat İli Patates Üretim Alanlarında Patates Yumrularında Enfeksiyon Oluşturan Virüslerin Moleküler Olarak Tespiti

Year 2024, Volume: 27 Issue: 5, 1061 - 1068

Afide Merve Engür Şerife Topkaya

https://doi.org/10.18016/ksutarimdoga.vi.1435134

Abstract

Tokat ilinde patates yetiştiriciliği yapılan Niksar, Erbaa ve Artova ilçelerindeki patates üretim alanlarından virüs belirtisi gösteren patates bitkilerinden 91 adet yumru örneği toplanmıştır. Yumru örnekleri, potato virus Y (PVY), potato virus S (PVS), potato virus X (PVX) ve potato leafroll virus (PLRV) etmenlerinin varlığını tespit etmek amacıyla RT-PCR testine tabi tutulmuştur. Test edilen yumru örneklerinin 68 tanesinde (%74.72) tekli veya karışık virüs enfeksiyonun varlığı tespit edilmiştir. Çimlendirilen 91 yumrunun 67’si PVY (%73.62), 6’sı PVS (%6.59) ve 1 tanesi de PLRV (%1.09) ile enfekteli bulunmuştur. Ayrıca PVS ile enfekteli bulunan yumrulardan 5 tanesinde PVY+PVS karışık enfeksiyonu belirlenirken, PLRV ile enfekteli yumruda da PVY enfeksiyonu tespit edilmiştir. Tokat ilinde farklı ilçelerden toplanan yumrularda en yaygın PVY görülürken bunu PVS’ etmeninin izlediği belirlenmiştir. Tohumluk olarak kullanılan enfekteli yumrular yıldan yıla verimde ciddi kayıplara neden olmaktadır. Virüsten ari ve sertifikalı tohumlukların kullanılması viral kaynaklı kaybın önüne geçilmesinde en önemli faktördür.

Keywords

Patates yumru PLRV PVS PVX PVY RT-PCR

Supporting Institution

Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Projeler Koordinatörlüğü

Project Number

2018/50

References

  • Arlı-Sökmen, M., Ayan, A.K., & Şevik, M.A. (2005). Trabzon ve Bayburt illerinde tohumluk patates (Solanum tuberosum L.) yumrularında belirlenen virüsler. OMÜ Ziraat Fakültesi Dergisi, 20 (3), 23–26. https://dergipark.org.tr/tr/download/article-file/187576
  • Astruc, N., Marcos, J.F., Macquaire, G., Candresse, T. & Pallas, V. (1996). Studies on the diagnosis of hop stunt viroid in fruit trees: identification of new hosts and application of nucleic acid extraction procedure based on non-organic solvents. European Journal of Plant Pathology, 102, 837–846. https://doi.org/10.1007/BF01877053
  • Bostan, H. & Haliloğlu, K. (2004). Distribution of PLRV, PVS, PVX, and PVY (PVYN, PVY0 and PVYC) in the seed potato tubers in Turkey. Pakistan Journal of Biological Sciences, 7(7), 1140-1143. https://doi: 10.3923/pjbs.2004.1140.1143
  • Bostan, H., Guclu, C., Ozturk, E., I, Ozdemir & Ilbagi, H. (2006). Influence of aphids on the epidemiology of potato virus diseases (PVY, PVS and PLRV) in the high altitude areas of Turkey. Pakistan Journal of Biological Sciences, 9, 759-765. https://doi: 10.3923/pjbs.2006.759.765
  • Bostan, H & Peker, P. K. (2009). The feasibility of tetraplex RT-PCR in the determination of PVS, PLRV, PVX and PVY from dormant potato tubers. African Journal of Biotechnology, 8 (17), 4043-4047 Doi:10.4314/AJB.V8I17.62127
  • Çalı, S. & Yalçın, N. (1991). İthal edilmiş tohumluk patateslerde önemli virüs hastalıklarının DAS-ELISA ve diğer yöntemlerle araştırılması (Bildiriler). VI. Türkiye Fitopatoloji Kongresi, İzmir, Türkiye, 7-11 Ekim 1991, ss. 333–336.
  • Çıtır, A. (1982). Erzurum ve çevresinde tohumluk patateslerdeki virus hastalıkları ve bunların tanılanması üzerinde bazı araştırmalar. Doğa Bilim Dergisi, 6(3), 99-109.
  • Devaux, A., Kromann, P. & Ortiz, O. (2014). Potatoes for sustainable global food security. Potato Research, 57, 185–199. DOI 10.1007/s11540-014-9265-1
  • Duan, G., Zhan, F., Du, Z., Ho, S.Y.W. & Gao, F. (2018). Europe was a hub for the global spread of potato virus S in the 19th century. Virology, 525, 200–204. https://doi.org/10.1016/j.virol.2018.09.022
  • Gümüş, M. & Erkan, S. (1998). Ayvalık ve Altınova yörelerinde üretilen patates çeşitlerinin yumrularında bulunan virüslerin belirlenmesi üzerinde araştırmalar (Bildiriler). VIII. Türkiye Fitopatoloji Kongresi, Ankara, Türkiye, 21-25 Eylül 1998, ss. 348–350.
  • Güner, Ü. & Yorgancı, Ü. (2006). Niğde ve Nevşehir İlleri patates ekiliş alanlarında saptanan viral etmenler. Bitki Koruma Bülteni, 46 (1-4), 35-49. https://dergipark.org.tr/tr/download/article-file/ 41538
  • Jones, E.D. (1988). A current assessment of in vitro culture and other rapid multiplication methods in North America and Europe. Am. Potato J., 65, 209-220. https://doi.org/10.1007/BF02854453
  • Kadakoğlu, B., & Karlı, B., (2022). Afyonkarahisar İlinde Patates Üretiminin Ekonomik Analizi. KSÜ Tarım ve Doğa Dergisi 25 (3), 581-588. https://doi.org/10.18016/ksutarimdoga.vi.947387.
  • Kökten, M. (2007). Ödemiş bölgesinde üretimi yapılan patates yumrularında PVY, PVX, PVS ve PLRV virüslerinin RT-PCR yöntemiyle saptanması (Tez no 200661). [Yüksek lisans tezi. Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Ana Bilim Dalı]. Aydın. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Kreuze, J.F., Souza-Dias, J.A.C., Jeevalatha, A., Figueira, A.R., Valkonen, J.P.T. & Jones, R.A.C. (2020). Viral diseases in Potato. Pages 389-430 in: In The Potato Crop; Campos, H., Ortiz, O., (Eds), Springer, Cham, Switzerland. https://doi.org/ 10.1007/978-3-030-28683-5_11.
  • Lesley, T. & Michael, E.T. (2020). Potato virus Y emergence and evolution from the andes of south america to become a major destructive pathogen of potato and other solanaceous crops worldwide. Viruses, 12, 1–14. https://doi.org/10.3390/ v12121430
  • Mao, Y., Sun, X., Shen, J., Gao, F., Qiu, G., Wang, T., Nie, X., Zhang, W., Gao, Y., & Bai, Y. (2019). Molecular Evolutionary Analysis of Potato Virus Y Infecting Potato Based on the VPg Gene. Frontiers in microbiology, 10, 1708. https://doi.org/10.3389/ fmicb.2019.01708
  • Slack, S.A. (1995). Potato viruses with some implications for production and processing in the United States: a history of problems and solutions. Summa Phytopathologica, 21, 273-275.
  • Spiegel, S. & Martin, R.P. (1993). Improved detection of potato leafroll virus in dormant potato tubers and micro tubers by the polymerase chain reaction and ELISA. Annals of Applied Biology, 121, 493-500. https://doi.org/10.1111/j.1744-7348.1993. tb04052.x
  • Topkaya, S. (2020). Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province. Journal of Agricultural Faculty of Gaziosmanpasa University, 37 (1), 53-59. doi:10.13002/jafag4685.
  • Topkaya, Ş., Çelik, A., Santosa, A.I. & Jones, R.A.C. (2023). Molecular Analysis of the Global Population of Potato Virus S Redefines Its Phylogeny, and Has Crop Biosecurity Implications.Viruses, 15, 1104. https://doi.org/ 10.3390/v15051104
  • Zhang, W., Bai, Y.Q., Gao, Y.L., Sheng, Y., Fan, G.Q. & Gen, H.W. (2010). A survey on occurrence frequencies of potato viruses in major potato producing provinces in China. Heilongjiang Agric. Sci., 4, 71–73. https://doi.org/10.1016/j.matlet. 2009.10.009
There are 22 citations in total.

Details

Primary Language Turkish
Subjects Phytopathology
Journal Section RESEARCH ARTICLE
Authors

Afide Merve Engür 0000-0002-5874-4016

Şerife Topkaya 0000-0002-0095-474X

Project Number 2018/50
Early Pub Date July 2, 2024
Publication Date
Submission Date February 11, 2024
Acceptance Date April 30, 2024
Published in Issue Year 2024Volume: 27 Issue: 5

Cite

APA Engür, A. M., & Topkaya, Ş. (2024). Tokat İli Patates Üretim Alanlarında Patates Yumrularında Enfeksiyon Oluşturan Virüslerin Moleküler Olarak Tespiti. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1061-1068. https://doi.org/10.18016/ksutarimdoga.vi.1435134
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