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Topraktan İzole Edilen Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Türünün Morfolojisi ve Moleküler Filogenisi

Year 2025, Volume: 28 Issue: 4, 914 - 929

Naciye Gülkız Şenler , İsmail Yıldız , Hilal Ural , Rıfat Bircan

https://doi.org/10.18016/ksutarimdoga.vi.1590418

Abstract

Bu çalışmada Tekirdağ (Türkiye) topraklarından hazırlanan kültürlerde birbiri ardınca ortaya çıkan iki Blepharisma popülasyonu gözlendi. Bu popülasyonlara ait hücre ve kistler morfolojik ve morfometrik olarak incelenmiş ve SSU rDNA gen dizisine dayanarak filogenetik konumları belirlenmiştir. Her iki popülasyon da morfometrik olarak birbirine çok benzerdi, ancak toprak kültürlerinde gözlenen ilk popülasyon olan popülasyon 1, esnek olmayan ve sert olan piriform biçime sahipken, popülasyon 1'in kaybolmasının ardından gözlenen popülasyon 2 ise, esnek, kırılgan, mızrak biçimindedir. Ayrıca, her iki popülasyonun hücre genişliği/uzunluğu oranlarının istatistiksel olarak birbirinden farklı olduğu bulunmuştur. Bununla birlikte, her iki popülasyonun SSU rDNA nükleotid dizileri birbirlerine ve daha önce tanımlanan diğer B. hyalinum popülasyonlarına çok benzediğinden, bu çalışmada gözlemlenen Blepharisma popülasyonlarının B. hyalinum'un morfotipleri olduğu sonucuna varıldı. Gözlenen morfolojik farkların çevresel faktörlere ve kültürlerdeki hücrelerin farklı yaşam evrelerinden kaynaklanabileceği varsayılmıştır.Blepharisma popülasyonlarının SSU rDNA nükleotid dizilerinin karşılaştırılması ve filogenetik analizler bazı farklı türlere ait nükleotid dizilerinin aynı ya da çok benzer olduklarını göstermiştir. Bu kaotik durumun açıklığa kavuşturulması için Blepharisma popülasyonları üzerinde ayrıntılı morfolojik incelemelere dayalı çok sayıda moleküler veriye ihtiyaç duyulmaktadır.

Keywords

Blepharisma hyalinum Morfoloji Dinlenme kisti Filogeni Türkiye

Project Number

TÜBİTAK 221Z208

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Morphology and Molecular Phylogeny of Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Isolated from Soil

Year 2025, Volume: 28 Issue: 4, 914 - 929

Naciye Gülkız Şenler , İsmail Yıldız , Hilal Ural , Rıfat Bircan

https://doi.org/10.18016/ksutarimdoga.vi.1590418

Abstract

In this study, two successive populations of Blepharisma were observed in cultures prepared from soil samples collected from Tekirdağ (Türkiye). The cells and cysts belonging to these populations were examined morphologically and morphometrically, and their phylogenetic positions were determined based on SSU rDNA gene sequences. Both populations were morphometrically very similar to each other, but population 1, the initial population observed in soil cultures, had a pyriform body that was inflexible and rigid while population 2, observed subsequent to the disappearance of population 1, exhibited a flexible, fragile, lanceolate body. In addition, the cell width/length ratios of both populations were found to be statistically different from each other. However, since the SSU rDNA nucleotide sequences of both populations are very similar to each other and to other B. hyalinum populations described previously, it is concluded that the observed Blepharisma populations represent different morphotypes of B. hyalinum. It has been hypothesised that the observed morphological differences may result from diet, environmental factors, and variations in the life stages of the cultured cells. Comparison of SSU rDNA nucleotide sequences of Blepharisma populations and phylogenetic analyses showed that the nucleotide sequences of some different species are identical or very similar. To clarify this chaotic situation, extensive molecular data based on detailed morphological studies on Blepharisma populations are necessary.

Keywords

Blepharisma hyalinum Morphology Resting cyst Phylogeny Türkiye

Project Number

TÜBİTAK 221Z208

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  • Al-Rasheid, K. A. (1999). Records of marine interstitial hymenostomatid ciliates from the Jubail Marine Wildlife Sanctuary, Saudi Arabia. Qatar Univ. Sci. J., 18, 123-130.
  • Al-Rasheid, K. A. S., Nilsson, J. R. & Larsen, H. F. (2001). Blepharisma intermedium Padmavathi, 1959 (Ciliophora: Heterotrichida) from Al-Hassa inland hypersaline oassis Saudi Arabia. Acta Parasitologica, 40, 63-69.
  • Asadullayeva, E. & Alekperov, İ. (2007). Free-living ciliates of the Anzali Wetland of the Caspian Sea. Turkish Journal of Zoology, 31(2), 143-149.
  • Ayres, D. L., Darling, A., Zwickl, D. J., Beerli, P., Holder, M. T., Lewis, P. O., Huelsenbeck, J. P., Ronquist, F., Swofford, D. L., Cummings, M. P., Rambaut, A. & Suchard, M. A. (2012). BEAGLE: an application programming interface and high-performance computing library for statistical phylogenetics. Systematic Biology, 61(1), 170-173. https//doi.org/10.1093/sysbio/syr100
  • Bhandary, A. V. (1962). Taxonomy of genus Blepharisma with special reference to Blepharisma undulans. Journal of Protozoology, 9, 435-442. https//doi.org/10.1111/j.1550-7408.1962.tb02649.x
  • Çapar, S. (2007). Checklist for ciliate species (Protozoa, Ciliophora) living in Turkish inland waters and flooded zones. E.Ü. Journal of Fisheries & Aquatic Sciences, 24, 207-212.
  • Cavaleiro, J., Fernandes, N. M., da Silva-Neto, I. D. & Soares, C. A. G. (2018). Resting cysts of the pigmented ciliate Blepharisma sinuosum Sawaya, 1940 (Ciliophora: Heterotrichea). Journal of Eukaryot Microbiology, 65(3), 422-426. https//doi.org/10.1111/jeu.12483
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  • Chi, Y., Chen, X., Li, Y., Wang, C., Zhang, T., Ayoub, A., Warren, A., Song, W. & Wang, Y. (2021). New contributions to the phylogeny of the ciliate class Heterotrichea (Protista, Ciliophora): analyses at family-genus level and new evolutionary hypotheses. Science China-Life Sciences, 64(4), 606-620. https//doi.org/10.1007/s11427- 020-1817-5
  • Darriba, D., Taboada, G. L., Doallo, R. & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(8), 772. https//doi.org/10.1038/nmeth.2109
  • Dragesco, J. (1970). Ciliés libres de Cameroun. Ann. Fac. Sci., Yaounde, Cameroun.
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Details

Primary Language English
Subjects Microbial Genetics, Microbiology (Other)
Journal Section RESEARCH ARTICLE
Authors

Naciye Gülkız Şenler 0000-0002-5035-0880

İsmail Yıldız 0000-0001-9986-0358

Hilal Ural 0009-0004-9928-7626

Rıfat Bircan 0000-0001-5291-8620

Project Number TÜBİTAK 221Z208
Early Pub Date June 11, 2025
Publication Date
Submission Date November 25, 2024
Acceptance Date May 21, 2025
Published in Issue Year 2025Volume: 28 Issue: 4

Cite

APA Şenler, N. G., Yıldız, İ., Ural, H., Bircan, R. (2025). Morphology and Molecular Phylogeny of Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Isolated from Soil. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 914-929. https://doi.org/10.18016/ksutarimdoga.vi.1590418
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