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Influences of Light-Emitting Diodes (LEDs) and Culture Media on Micropropagation of Native Hemp (Cannabis sativa) Population from Türkiye

Year 2025, Volume: 28 Issue: 4, 1071 - 1082

Cennet Yaman , Güngör Yılmaz , Serkan Uranbey

https://doi.org/10.18016/ksutarimdoga.vi.1596868

Abstract

Light-emitting diodes (LEDs) have historically been the standard for supplemental lighting in industrial hemp (Cannabis sativa) cultivation in a greenhouse, but there is a lack of scientific understanding in C. sativa regarding different media under various LEDs in vitro conditions. The aim of this study was to determine the top-performing LEDs and nutrient media for micropropagation of C. sativa. In this study, two different spectra (white and red LEDs) and different media [MS, B5, MS+B5, ½ MS, ½ B5, ½ MS + ½ B5, and control (without medium)] were used, maintaining constant light photoperiod. The diversity of light spectra and nutrient media showed statistically significant differences for the growth parameters of C. sativa. Red LED provided an extraordinarily high shoot length of 7.37 cm, whereas white LED exhibited the highest root length with 6.57 cm. Also, nutrient media were found to be effective on shoot growth in full-strength media, while on root growth in half-strength media. Among the plant growth parameters, the number of shoots and nodes per plant is of great importance for clonal propagation. B5 medium under red LED provided an extraordinarily high shoot number with 4.64, whereas MS medium under white LED exhibited the highest node number with 5.87. Each LED source required the respective medium adjustment for the best results of the desired parameters. In this respect, the study may be advantageous for the selection of the appropriate nutrient medium and led spectrum in micropropagation of C. sativa.

Keywords

Micropropagation Industrial hemp Cannabis sativa Red LED Culture media

Ethical Statement

The authors of the articles declare that they have no conflict of interest.

Thanks

The authors are grateful to the Hemp Research Institute of Yozgat Bozok University in Türkiye for helping to procure the seeds.

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Türkiye'deki Yerel Kenevir (Cannabis sativa) Popülasyonunun Mikroçoğaltımı Üzerinde Işık Yayan Diyotların (LED'ler) ve Kültür Ortamlarının Etkileri

Year 2025, Volume: 28 Issue: 4, 1071 - 1082

Cennet Yaman , Güngör Yılmaz , Serkan Uranbey

https://doi.org/10.18016/ksutarimdoga.vi.1596868

Abstract

Işık yayan diyotlar (LED'ler), tarihsel olarak serada endüstriyel kenevir (Cannabis sativa) yetiştiriciliğinde ek aydınlatma için standart olmuştur, ancak C. sativa'da in vitro koşullarında çeşitli LED'ler altında farklı ortamlar konusunda bilimsel anlayış eksikliği vardır. Bu çalışmanın amacı, C. sativa'nın mikro çoğaltılması için en iyi performans gösteren LED'leri ve besin ortamlarını belirlemektir. Bu çalışmada, sabit ışık fotoperiyodu korunarak iki farklı spektrum (beyaz ve kırmızı LED'ler) ve farklı ortamlar [MS, B5, MS + B5, ½ MS, ½ B5, ½ MS + ½ B5 ve kontrol (ortamsız)] kullanıldı. Işık spektrumlarının ve besin ortamlarının çeşitliliği, C. sativa'nın büyüme paarametreleri için istatistiksel olarak anlamlı farklılıklar gösterdi. Kırmızı LED, 7,37 cm ile olağanüstü yüksek bir sürgün uzunluğu sağlarken, beyaz LED 6,57 cm ile en yüksek kök uzunluğunu sergiledi. Ayrıca, besin ortamlarının tam güçteki ortamlarda sürgün büyümesinde, yarı güçteki ortamlarda ise kök büyümesinde etkili olduğu bulunmuştur. Bitki büyüme parametreleri arasında, bitki başına sürgün ve nod sayıları klonal çoğaltım için büyük önem taşımaktadır. Kırmızı LED altında B5 ortamı 4.64 ile olağanüstü yüksek bir sürgün sayısı sağlarken, beyaz LED altında MS ortamı 5.87 ile en yüksek nod sayısını sergilemiştir. İstenilen parametrelerin en iyi sonuçları için her bir LED kaynağı ilgili ortam ayarlamasını gerektirmiştir. Bu açıdan, çalışma C. sativa'nın mikroçoğaltımında uygun besin ortamının ve LED spektrumunun seçilmesi için avantajlı olabilir.

Keywords

Endüstriyel kenevir Kırmızı LED Beyaz LED Kültür ortamı Cannabis sativa

References

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  • Adhikary, D., Kulkarni, M., El-Mezawy, A., Mobini, S., Elhiti, M., Gjuric, R., ... & Bhowmik, P. (2021). Medical cannabis and industrial hemp tissue culture: present status and future potential. Frontiers in Plant Science, 12, 627240.
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  • Clarke, R. C., & Merlin, M. D. (2016). Cannabis domestication, breeding history, present-day genetic diversity, and future prospects. Critical Reviews in Plant Sciences, 35(5-6), 293-327.
  • Davis, P. A., & Burns, C. (2016). Photobiology in protected horticulture. Food and Energy Security, 5(4), 223-238.
  • dos Reis Oliveira, T., Aragão, V. P. M., Moharana, K. C., Fedosejevs, E., do Amaral, F. P., Sousa, K. R., ... & Santa-Catarina, C. (2020). Light spectra affect the in vitro shoot development of Cedrela fissilis Vell.(Meliaceae) by changing the protein profile and polyamine contents. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1868(12), 140529.
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  • Fike, J. H., Darby, H., Johnson, B. L., Smart, L., & Williams, D. W. (2020). Industrial hemp in the USA: a brief synopsis bt- sustainable agriculture reviews 42: hemp production and applications; Crini, G., Lichtfouse, E., Eds.; Springer International Publishing: Cham, Switzerland, pp. 89–109.
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There are 67 citations in total.

Details

Primary Language English
Subjects Plant Biotechnology, Agricultural Biotechnology (Other)
Journal Section RESEARCH ARTICLE
Authors

Cennet Yaman 0000-0002-2364-8171

Güngör Yılmaz 0000-0003-0070-5484

Serkan Uranbey 0000-0002-0312-8099

Early Pub Date June 10, 2025
Publication Date
Submission Date December 5, 2024
Acceptance Date April 17, 2025
Published in Issue Year 2025Volume: 28 Issue: 4

Cite

APA Yaman, C., Yılmaz, G., & Uranbey, S. (2025). Influences of Light-Emitting Diodes (LEDs) and Culture Media on Micropropagation of Native Hemp (Cannabis sativa) Population from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 1071-1082. https://doi.org/10.18016/ksutarimdoga.vi.1596868
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