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Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği

Year 2025, Volume: 28 Issue: 4, 1083 - 1094

Şeyma Önlü

https://doi.org/10.18016/ksutarimdoga.vi.1614168

Abstract

Bu çalışmanın amacı, absisik asitin (ABA) Hypericum perforatum'un in vitro kalluslarının kolinesteraz enzim aktiviteleri ve biyoaktif bileşenleri üzerine etkisini araştırmaktır. Kalluslar, 0.5 mg L-1 thidiazuron (TDZ) ve 0.5 mg L-1 indol bütirik asit (IBA) içeren Linsmaier Skoog (LS)/B5 besiyerinden elde edildi. Doku kültüründe elde edilen kalluslara farklı absisik asit (0,01 mg L-1, 0,05 mg L-1 ve 0,1 mg L-1) uygulamaları yapıldı ve %80'lik metanolde ekstrakte edildi. Hispidulin, fumarik asit, akasetin, epikateşin, naringenin bileşikleri Sıvı Kromatografisi-Yüksek Çözünürlüklü Kütle Spektrometrisi (LC-HRMS) ile analiz edildi. Kallus örneklerinde en yüksek epikateşin (1.09 mg L-1) 0.05 mg L-1 ABA'da bulunurken, fumarik asit (2.30 mg L-1) ve hispidulin (0.78 mg L-1) bileşikleri en yüksek 0.1 mg L-1 ABA içerenlerde tespit edildi. Sadece 0.01 mg L-1 ABA içeren ortamda akasetin (0.10 mg L-1) üretilirken, 0.05 mg L-1 ABA içeren besin ortamında naringenin (0.01 mg L-1) üretildi. En güçlü Asetilkolinesteraz (AchE) (0.207±0.012) ve Bütirilkolinesteraz BChE (0.243±0.019) inhibisyon aktivitesinin 0.1 mg L -1 ABA uygulamasıyla olduğu ortaya çıktı. Bu çalışma, ABA'nın H.perforatum'un kallus kültüründe uyarıcı olarak kullanılabileceğini ve ilgili metabolitlerin miktarlarının elisitör aracılığıyla değiştirilebileceğini göstermiştir.

Keywords

Absisik asit Kallus üretimi Hispidulin LC-HRMS Kolinesteraz inhibisyonu

Supporting Institution

Muş Alparslan Üniversitesi, Bilimsel Araştırma proje birimi

Project Number

18-FEF-4901-02

References

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Efficacy of Exogenous Abscisic Acid on Cholinesterase Enzyme Activity and Phenolic Compound Variability in Hypericum perforatum calli

Year 2025, Volume: 28 Issue: 4, 1083 - 1094

Şeyma Önlü

https://doi.org/10.18016/ksutarimdoga.vi.1614168

Abstract

The aim of this study was to investigate the effect of abscisic acid (ABA) on cholinesterase enzyme activities and bioactive components of in vitro calli of Hypericum perforatum. Calli were obtained from Linsmaier Skoog (LS)/B5 medium containing 0.5 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 indole butyric acid (IBA). Different abscisic acid (0.01 mg L-1, 0.05 mg L-1, and 0.1 mg L-1) applications were performed on the calli obtained in tissue culture and extracted in 80% methanol. Hispidulin, fumaric acid, acacetin, epicatechin, and naringenin compounds were analyzed in Liquid chromatography–high–resolution mass spectrometry (LC-HRMS). While epicatechin (1.09 mg L-1) was found at the highest 0.05 mg L-1 ABA in callus samples, fumaric acid (2.30 mg L-1) and hispidulin (0.78 mg L-1) compounds were detected in the highest containing of 0.1 mg L-1 ABA. Acacetin (0.10 mg L-1) was produced in only 0.01 mg L-1 ABA, whereas naringenin (0.01 mg L-1) was produced in medium containing 0.05 mg L-1 ABA. The strongest Acetylcholinesterase (AChE) (0.207±0.012) and Butyrylcholinesterase (BChE) (0.243±0.019) inhibition activity was shown the best with 0.1 mg L-1 ABA application. This study has shown that ABA can be used as an elicitor in callus culture of H. perforatum, and the amounts of related metabolites can be changed via elicitors

Keywords

Abscisic acid Callus Hispidulin LC-HRMS Cholinesterase inhibition

Project Number

18-FEF-4901-02

References

  • Abyadeh, M., Gupta, V., Paulo, J. A., Mahmoudabad, A. G., Shadfar, S., Mirshahvaladi, S., Gupta, V., Nguyen, C. T., Finkelstein, D. I., & You, Y. (2024). Amyloid-beta and tau protein beyond Alzheimer’s disease. Neural Regeneration Research, 19(6), 1262-1276. https://doi.org/https://doi.org/10.4103/1673-5374.386406
  • Al-Khayri, J. M., Narasimha, S. W., Vennapusa, A. R., Nagella, P., Shehata, W. F., & Al-Mssallem, M. Q. (2024). Biotechnological approaches for the production of hypericin and other important metabolites from the genus Hypericum. Plant Cell, Tissue and Organ Culture (PCTOC), 156(3), 100. https://doi.org/https://doi.org/10.1007/s11240-024-02723-7
  • Altıntaş, M. Y. (2025). Hypericum calycinum L. Türü Üzerine Detaylı Anatomik Çalışma. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 672-682. https://doi.org/https://doi.org/10.1016/j.indcrop.2012.07.017
  • Altun, M. L., Yılmaz, B. S., Orhan, I. E., & Citoglu, G. S. (2013). Assessment of cholinesterase and tyrosinase inhibitory and antioxidant effects of Hypericum perforatum L.(St. John's wort). Industrial Crops and Products, 43, 87-92. https://doi.org/https://doi.org/10.1016/j.indcrop.2012.07.017
  • Bal, A., Özen, H. Ç., Tural, B., & Ertaş, E. (2022). The effects of different concentrations of foliar applied chitosan, iron oxide and chitosan-coated iron oxide nanoparticles on the secondary metabolites of Hypericum triquetrifolium Turra. during full bloom. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(4), 811-818. https://doi.org/ https://doi.org/10.18016/ksutarimdoga.vi.882856
  • Barnes, J., Anderson, L. A., & Phillipson, J. D. (2001). St John's wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. Journal of pharmacy and pharmacology, 53(5), 583-600. https://doi.org/https://doi.org/10.1211/0022357011775910
  • Boga, M., Ertas, A., Eroglu-Ozkan, E., Kizil, M., Ceken, B., & Topcu, G. (2016). Phytochemical analysis, antioxidant, antimicrobial, anticholinesterase and DNA protective effects of Hypericum capitatum var. capitatum extracts. South African Journal of Botany, 104, 249-257. https://doi.org/https://doi.org/10.1016/j.sajb.2016.02.204
  • Bombardelli, E., & Morazzoni, P. (1995). Hypericum perforatum. Fitoterapia, 66, 43-68.
  • Bruneton, J. (1999). Pharmacognosy, phytochemistry, medicinal plants. Intercept Ltd.: Hampshire, UK,. Chulasiri, M., Bunyapraphatsara, N., & Moongkarndi, P. (1992). Mutagenicity and antimutagenicity of hispidulin and hortensin, the flavonoids from Millingtonia hortensis L. Environmental and Molecular Mutagenesis, 20(4), 307-312. https://doi.org/https://doi.org/10.1002/em.2850200409
  • Crockett, S. L., & Robson, N. K. (2011). Taxonomy and chemotaxonomy of the genus Hypericum. Medicinal and aromatic plant science and biotechnology, 5(Special Issue 1), 1.
  • Ebadollahi, R., Jafarirad, S., Kosari-Nasab, M., & Mahjouri, S. (2019). Effect of explant source, perlite nanoparticles and TiO2/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum. Scientific Reports, 9(1), 12998. https://doi.org/ https://doi.org/10.1038/s41598-019-49504-3
  • Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95. https://doi.org/https://doi.org/10.1016/0006-2952(61)90145-9
  • Ferreira, P. G., Ferraz, A. C., Figueiredo, J. E., Lima, C. F., Rodrigues, V. G., Taranto, A. G., Ferreira, J. M. S., Brandão, G. C., Vieira-Filho, S. A., & Duarte, L. P. (2018). Detection of the antiviral activity of epicatechin isolated from Salacia crassifolia (Celastraceae) against Mayaro virus based on protein C homology modelling and virtual screening. Archives of virology, 163, 1567-1576. https://doi.org/https://doi.org/10.1007/s00705-018-3774-1
  • Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Hagège, D., Courtois, D., & Joseph, C. (2013). The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L. Plant Cell, Tissue and Organ Culture (PCTOC), 113, 25-39. https://doi.org/https://doi.org/10.1007/s11240-012-0248-0
  • Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Joseph, C., & Hagege, D. (2007). Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant cell, tissue and organ culture, 89, 1-13. https://doi.org/https://doi.org/10.1007/s11240-007-9203-x
  • Gadzovska, S., Maury, S., Ounnar, S., Righezza, M., Kascakova, S., Refregiers, M., Spasenoski, M., Joseph, C., & Hagège, D. (2005). Identification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiology and Biochemistry, 43(6), 591-601. https://doi.org/https://doi.org/10.1016/j.plaphy.2005.05.005
  • Gil, B., Sanz, M., Terencio, M., Ferrandiz, M., Bustos, G., Paya, M., Gunasegaran, R., & Alcaraz, M. (1994). Effects of flavonoids on Naja naja and human recombinant synovial phospholipases A2 and inflammatory responses in mice. Life sciences, 54(20), PL333-338. https://doi.org/https://doi.org/10.1016/0024-3205(94)90021-3
  • Gjureci, B., Todorovska, M., Stanoeva, J. P., Tusevski, O., & Simic, S. G. (2025). Elicitation of Hypericum perforatum L. hairy root cultures with salicylic acid and jasmonic acid enhances the production of phenolic compounds and naphthodianthrones with biological activities. Plant Cell, Tissue and Organ Culture (PCTOC), 160(3), 61. https://doi.org/https://doi.org/10.1007/s11240-025-03005-6
  • Inal, E., Ulusoy, Ş., Yapar, E. A., Gürer, E. S., Yalçın, Ş., & Kartal, M. (2022). Investigation of resveratrol and phenolic compounds of ethnomedicinal plant Polygonum cognatum Meissn. collected from Sivas. J Res Pharm, 26(6), 1752-1757. https://doi.org/https://doi.org/10.29228/jrp.265
  • Kakouri, E., Trigas, P., Daferera, D., Skotti, E., Tarantilis, P. A., & Kanakis, C. (2023). Chemical characterization and antioxidant activity of nine Hypericum species from Greece. Antioxidants, 12(4), 899. https://doi.org/https://doi.org/10.3390/antiox12040899
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There are 41 citations in total.

Details

Primary Language Turkish
Subjects Plant Biotechnology
Journal Section RESEARCH ARTICLE
Authors

Şeyma Önlü 0000-0003-2005-1019

Project Number 18-FEF-4901-02
Early Pub Date June 10, 2025
Publication Date
Submission Date January 6, 2025
Acceptance Date May 17, 2025
Published in Issue Year 2025Volume: 28 Issue: 4

Cite

APA Önlü, Ş. (2025). Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 1083-1094. https://doi.org/10.18016/ksutarimdoga.vi.1614168
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