Araştırma Makalesi
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Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği

Yıl 2025, Cilt: 28 Sayı: 4, 1083 - 1094

Şeyma Önlü

https://doi.org/10.18016/ksutarimdoga.vi.1614168

Öz

Bu çalışmanın amacı, absisik asitin (ABA) Hypericum perforatum'un in vitro kalluslarının kolinesteraz enzim aktiviteleri ve biyoaktif bileşenleri üzerine etkisini araştırmaktır. Kalluslar, 0.5 mg L-1 thidiazuron (TDZ) ve 0.5 mg L-1 indol bütirik asit (IBA) içeren Linsmaier Skoog (LS)/B5 besiyerinden elde edildi. Doku kültüründe elde edilen kalluslara farklı absisik asit (0,01 mg L-1, 0,05 mg L-1 ve 0,1 mg L-1) uygulamaları yapıldı ve %80'lik metanolde ekstrakte edildi. Hispidulin, fumarik asit, akasetin, epikateşin, naringenin bileşikleri Sıvı Kromatografisi-Yüksek Çözünürlüklü Kütle Spektrometrisi (LC-HRMS) ile analiz edildi. Kallus örneklerinde en yüksek epikateşin (1.09 mg L-1) 0.05 mg L-1 ABA'da bulunurken, fumarik asit (2.30 mg L-1) ve hispidulin (0.78 mg L-1) bileşikleri en yüksek 0.1 mg L-1 ABA içerenlerde tespit edildi. Sadece 0.01 mg L-1 ABA içeren ortamda akasetin (0.10 mg L-1) üretilirken, 0.05 mg L-1 ABA içeren besin ortamında naringenin (0.01 mg L-1) üretildi. En güçlü Asetilkolinesteraz (AchE) (0.207±0.012) ve Bütirilkolinesteraz BChE (0.243±0.019) inhibisyon aktivitesinin 0.1 mg L -1 ABA uygulamasıyla olduğu ortaya çıktı. Bu çalışma, ABA'nın H.perforatum'un kallus kültüründe uyarıcı olarak kullanılabileceğini ve ilgili metabolitlerin miktarlarının elisitör aracılığıyla değiştirilebileceğini göstermiştir.

Anahtar Kelimeler

Absisik asit Kallus üretimi Hispidulin LC-HRMS Kolinesteraz inhibisyonu

Destekleyen Kurum

Muş Alparslan Üniversitesi, Bilimsel Araştırma proje birimi

Proje Numarası

18-FEF-4901-02

Kaynakça

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  • Altun, M. L., Yılmaz, B. S., Orhan, I. E., & Citoglu, G. S. (2013). Assessment of cholinesterase and tyrosinase inhibitory and antioxidant effects of Hypericum perforatum L.(St. John's wort). Industrial Crops and Products, 43, 87-92. https://doi.org/https://doi.org/10.1016/j.indcrop.2012.07.017
  • Bal, A., Özen, H. Ç., Tural, B., & Ertaş, E. (2022). The effects of different concentrations of foliar applied chitosan, iron oxide and chitosan-coated iron oxide nanoparticles on the secondary metabolites of Hypericum triquetrifolium Turra. during full bloom. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(4), 811-818. https://doi.org/ https://doi.org/10.18016/ksutarimdoga.vi.882856
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  • Boga, M., Ertas, A., Eroglu-Ozkan, E., Kizil, M., Ceken, B., & Topcu, G. (2016). Phytochemical analysis, antioxidant, antimicrobial, anticholinesterase and DNA protective effects of Hypericum capitatum var. capitatum extracts. South African Journal of Botany, 104, 249-257. https://doi.org/https://doi.org/10.1016/j.sajb.2016.02.204
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Efficacy of Exogenous Abscisic Acid on Cholinesterase Enzyme Activity and Phenolic Compound Variability in Hypericum perforatum calli

Yıl 2025, Cilt: 28 Sayı: 4, 1083 - 1094

Şeyma Önlü

https://doi.org/10.18016/ksutarimdoga.vi.1614168

Öz

The aim of this study was to investigate the effect of abscisic acid (ABA) on cholinesterase enzyme activities and bioactive components of in vitro calli of Hypericum perforatum. Calli were obtained from Linsmaier Skoog (LS)/B5 medium containing 0.5 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 indole butyric acid (IBA). Different abscisic acid (0.01 mg L-1, 0.05 mg L-1, and 0.1 mg L-1) applications were performed on the calli obtained in tissue culture and extracted in 80% methanol. Hispidulin, fumaric acid, acacetin, epicatechin, and naringenin compounds were analyzed in Liquid chromatography–high–resolution mass spectrometry (LC-HRMS). While epicatechin (1.09 mg L-1) was found at the highest 0.05 mg L-1 ABA in callus samples, fumaric acid (2.30 mg L-1) and hispidulin (0.78 mg L-1) compounds were detected in the highest containing of 0.1 mg L-1 ABA. Acacetin (0.10 mg L-1) was produced in only 0.01 mg L-1 ABA, whereas naringenin (0.01 mg L-1) was produced in medium containing 0.05 mg L-1 ABA. The strongest Acetylcholinesterase (AChE) (0.207±0.012) and Butyrylcholinesterase (BChE) (0.243±0.019) inhibition activity was shown the best with 0.1 mg L-1 ABA application. This study has shown that ABA can be used as an elicitor in callus culture of H. perforatum, and the amounts of related metabolites can be changed via elicitors

Anahtar Kelimeler

Abscisic acid Callus Hispidulin LC-HRMS Cholinesterase inhibition

Proje Numarası

18-FEF-4901-02

Kaynakça

  • Abyadeh, M., Gupta, V., Paulo, J. A., Mahmoudabad, A. G., Shadfar, S., Mirshahvaladi, S., Gupta, V., Nguyen, C. T., Finkelstein, D. I., & You, Y. (2024). Amyloid-beta and tau protein beyond Alzheimer’s disease. Neural Regeneration Research, 19(6), 1262-1276. https://doi.org/https://doi.org/10.4103/1673-5374.386406
  • Al-Khayri, J. M., Narasimha, S. W., Vennapusa, A. R., Nagella, P., Shehata, W. F., & Al-Mssallem, M. Q. (2024). Biotechnological approaches for the production of hypericin and other important metabolites from the genus Hypericum. Plant Cell, Tissue and Organ Culture (PCTOC), 156(3), 100. https://doi.org/https://doi.org/10.1007/s11240-024-02723-7
  • Altıntaş, M. Y. (2025). Hypericum calycinum L. Türü Üzerine Detaylı Anatomik Çalışma. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 672-682. https://doi.org/https://doi.org/10.1016/j.indcrop.2012.07.017
  • Altun, M. L., Yılmaz, B. S., Orhan, I. E., & Citoglu, G. S. (2013). Assessment of cholinesterase and tyrosinase inhibitory and antioxidant effects of Hypericum perforatum L.(St. John's wort). Industrial Crops and Products, 43, 87-92. https://doi.org/https://doi.org/10.1016/j.indcrop.2012.07.017
  • Bal, A., Özen, H. Ç., Tural, B., & Ertaş, E. (2022). The effects of different concentrations of foliar applied chitosan, iron oxide and chitosan-coated iron oxide nanoparticles on the secondary metabolites of Hypericum triquetrifolium Turra. during full bloom. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(4), 811-818. https://doi.org/ https://doi.org/10.18016/ksutarimdoga.vi.882856
  • Barnes, J., Anderson, L. A., & Phillipson, J. D. (2001). St John's wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. Journal of pharmacy and pharmacology, 53(5), 583-600. https://doi.org/https://doi.org/10.1211/0022357011775910
  • Boga, M., Ertas, A., Eroglu-Ozkan, E., Kizil, M., Ceken, B., & Topcu, G. (2016). Phytochemical analysis, antioxidant, antimicrobial, anticholinesterase and DNA protective effects of Hypericum capitatum var. capitatum extracts. South African Journal of Botany, 104, 249-257. https://doi.org/https://doi.org/10.1016/j.sajb.2016.02.204
  • Bombardelli, E., & Morazzoni, P. (1995). Hypericum perforatum. Fitoterapia, 66, 43-68.
  • Bruneton, J. (1999). Pharmacognosy, phytochemistry, medicinal plants. Intercept Ltd.: Hampshire, UK,. Chulasiri, M., Bunyapraphatsara, N., & Moongkarndi, P. (1992). Mutagenicity and antimutagenicity of hispidulin and hortensin, the flavonoids from Millingtonia hortensis L. Environmental and Molecular Mutagenesis, 20(4), 307-312. https://doi.org/https://doi.org/10.1002/em.2850200409
  • Crockett, S. L., & Robson, N. K. (2011). Taxonomy and chemotaxonomy of the genus Hypericum. Medicinal and aromatic plant science and biotechnology, 5(Special Issue 1), 1.
  • Ebadollahi, R., Jafarirad, S., Kosari-Nasab, M., & Mahjouri, S. (2019). Effect of explant source, perlite nanoparticles and TiO2/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum. Scientific Reports, 9(1), 12998. https://doi.org/ https://doi.org/10.1038/s41598-019-49504-3
  • Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95. https://doi.org/https://doi.org/10.1016/0006-2952(61)90145-9
  • Ferreira, P. G., Ferraz, A. C., Figueiredo, J. E., Lima, C. F., Rodrigues, V. G., Taranto, A. G., Ferreira, J. M. S., Brandão, G. C., Vieira-Filho, S. A., & Duarte, L. P. (2018). Detection of the antiviral activity of epicatechin isolated from Salacia crassifolia (Celastraceae) against Mayaro virus based on protein C homology modelling and virtual screening. Archives of virology, 163, 1567-1576. https://doi.org/https://doi.org/10.1007/s00705-018-3774-1
  • Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Hagège, D., Courtois, D., & Joseph, C. (2013). The influence of salicylic acid elicitation of shoots, callus, and cell suspension cultures on production of naphtodianthrones and phenylpropanoids in Hypericum perforatum L. Plant Cell, Tissue and Organ Culture (PCTOC), 113, 25-39. https://doi.org/https://doi.org/10.1007/s11240-012-0248-0
  • Gadzovska, S., Maury, S., Delaunay, A., Spasenoski, M., Joseph, C., & Hagege, D. (2007). Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant cell, tissue and organ culture, 89, 1-13. https://doi.org/https://doi.org/10.1007/s11240-007-9203-x
  • Gadzovska, S., Maury, S., Ounnar, S., Righezza, M., Kascakova, S., Refregiers, M., Spasenoski, M., Joseph, C., & Hagège, D. (2005). Identification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiology and Biochemistry, 43(6), 591-601. https://doi.org/https://doi.org/10.1016/j.plaphy.2005.05.005
  • Gil, B., Sanz, M., Terencio, M., Ferrandiz, M., Bustos, G., Paya, M., Gunasegaran, R., & Alcaraz, M. (1994). Effects of flavonoids on Naja naja and human recombinant synovial phospholipases A2 and inflammatory responses in mice. Life sciences, 54(20), PL333-338. https://doi.org/https://doi.org/10.1016/0024-3205(94)90021-3
  • Gjureci, B., Todorovska, M., Stanoeva, J. P., Tusevski, O., & Simic, S. G. (2025). Elicitation of Hypericum perforatum L. hairy root cultures with salicylic acid and jasmonic acid enhances the production of phenolic compounds and naphthodianthrones with biological activities. Plant Cell, Tissue and Organ Culture (PCTOC), 160(3), 61. https://doi.org/https://doi.org/10.1007/s11240-025-03005-6
  • Inal, E., Ulusoy, Ş., Yapar, E. A., Gürer, E. S., Yalçın, Ş., & Kartal, M. (2022). Investigation of resveratrol and phenolic compounds of ethnomedicinal plant Polygonum cognatum Meissn. collected from Sivas. J Res Pharm, 26(6), 1752-1757. https://doi.org/https://doi.org/10.29228/jrp.265
  • Kakouri, E., Trigas, P., Daferera, D., Skotti, E., Tarantilis, P. A., & Kanakis, C. (2023). Chemical characterization and antioxidant activity of nine Hypericum species from Greece. Antioxidants, 12(4), 899. https://doi.org/https://doi.org/10.3390/antiox12040899
  • Karuppusamy, S. (2009). A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plants Res, 3(13), 1222-1239. https://doi.org/https://doi.org/10.5897/JMPR.9000026
  • Kavvadias, D., Sand, P., Youdim, K. A., Qaiser, M. Z., Rice‐Evans, C., Baur, R., Sigel, E., Rausch, W. D., Riederer, P., & Schreier, P. (2004). The flavone hispidulin, a benzodiazepine receptor ligand with positive allosteric properties, traverses the blood–brain barrier and exhibits anticonvulsive effects. British journal of pharmacology, 142(5), 811-820. https://doi.org/https://doi.org/10.1038/sj.bjp.0705828
  • Lema-Rumińska, J., Goncerzewicz, K., & Gabriel, M. (2013). Influence of abscisic acid and sucrose on somatic embryogenesis in cactus Copiapoa tenuissima Ritt. forma mostruosa. The Scientific World Journal, 2013(1), 513985. https://doi.org/ https://doi.org/10.1155/2013/513985
  • Lin, Y.-T., Wu, Y.-H., Tseng, C.-K., Lin, C.-K., Chen, W.-C., Hsu, Y.-C., & Lee, J.-C. (2013). Green tea phenolic epicatechins inhibit hepatitis C virus replication via cycloxygenase-2 and attenuate virus-induced inflammation. PloS one, 8(1), e54466. https://doi.org/https://doi.org/10.1371/journal.pone.0054466
  • Manivannan, A., Soundararajan, P., Park, Y. G., & Jeong, B. R. (2015). In vitro propagation, phytochemical analysis, and evaluation of free radical scavenging property of Scrophularia kakudensis Franch tissue extracts. BioMed Research International, 2015(1), 480564. https://doi.org/https://doi.org/10.1155/2015/480564
  • Murthy, H. N., Lee, E.-J., & Paek, K.-Y. (2014). Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture (PCTOC), 118, 1-16. https://doi.org/https://doi.org/10.1007/s11240-014-0467-7
  • Ozkan, E. E., Ozden, T. Y., Ozsoy, N., & Mat, A. (2018). Evaluation of chemical composition, antioxidant and anti-acetylcholinesterase activities of Hypericum neurocalycinum and Hypericum malatyanum. South African Journal of Botany, 114, 104-110. https://doi.org/ https://doi.org/10.1016/j.sajb.2017.10.022
  • Önlü, Ş. (2023, 11-13 OCTOBER). Investigation of phenolic compounds and elicited by jasmonate derivatives on in vitro samples of Hypericum perforatum L. 6th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2023) ANKARA.
  • Önlü, Ş., Yaman, C., Kurtul, E., Önlü, H., Bahadir-Acikara, Ö., Tusevski, O., Simic, S. G., & Özcan, S. (2025). Production of elicitor-induced phytochemicals in callus and shoot cultures of Hypericum heterophyllum. South African Journal of Botany, 177, 295-304. https://doi.org/https://doi.org/10.1016/j.sajb.2024.12.003
  • Pathania, S., & Singh, P. K. (2021). Analyzing FDA-approved drugs for compliance of pharmacokinetic principles: should there be a critical screening parameter in drug designing protocols? Expert Opinion on Drug Metabolism & Toxicology, 17(4), 351-354. https://doi.org/ https://doi.org/10.1080/17425255.2021.1865309
  • Shasmita, Behera, S., Mishra, P., Samal, M., Mohapatra, D., Monalisa, K., & Naik, S. K. (2023). Recent advances in tissue culture and secondary metabolite production in Hypericum perforatum L. Plant Cell, Tissue and Organ Culture (PCTOC), 154(1), 13-28. https://doi.org/https://doi.org/10.1007/s11240-023-02525-3
  • Song, R., Xia, Y., Zhao, Z., Yang, X., & Zhang, N. (2023). Effects of plant growth regulators on the contents of rutin, hyperoside and quercetin in Hypericum attenuatum Choisy. PloS one, 18(5), e0285134. https://doi.org/10.1371/dergi.pone.0285134
  • Suryawanshi, M. V., Gujarathi, P. P., Mulla, T., & Bagban, I. (2024). Hypericum perforatum: A comprehensive review on pharmacognosy, preclinical studies, putative molecular mechanism, and clinical studies in neurodegenerative diseases. Naunyn-Schmiedeberg's Archives of Pharmacology, 397(6), 3803-3818. https://doi.org/10.1007/s00210-023-02915-6
  • Sut, S., Dall'Acqua, S., Flores, G. A., Cusumano, G., Koyuncu, İ., Yuksekdag, O., Emiliani, C., Venanzoni, R., Angelini, P., & Selvi, S. (2025). Hypericum empetrifolium and H. lydium as Health Promoting Nutraceuticals: Assessing Their Role Combining In Vitro In Silico and Chemical Approaches. Food Science & Nutrition, 13(4), e70053. https://doi.org/https://doi.org/10.1002/fsn3.70053
  • Tusevski, O., Todorovska, M., Todorovska, I., Petreska Stanoeva, J., & Gadzovska Simic, S. (2024). Production of Phenylpropanoids, Naphthodianthrones and Antioxidant Status of Hypericum perforatum L. Transgenic Shoots. Horticulturae, 10(1), 59. https://doi.org/https://doi.org/10.3390/horticulturae10010059
  • Uçar, K., & Göktaş, Z. (2023). Biological activities of naringenin: A narrative review based on in vitro and in vivo studies. Nutrition Research. https://doi.org/https://doi.org/10.1016/j.nutres.2023.08.006
  • Vaičiukynė, M., Žiauka, J., Žūkienė, R., Vertelkaitė, L., & Kuusienė, S. (2019). Abscisic acid promotes root system development in birch tissue culture: a comparison to aspen culture and conventional rooting‐related growth regulators. Physiologia plantarum, 165(1), 114-122. https://doi.org/ https://doi.org/10.1111/ppl.12860
  • Xiao, C.-Y., Mu, Q., & Gibbons, S. (2020). The phytochemistry and pharmacology of Hypericum. Progress in the chemistry of organic natural products 112, 85-182. https://doi.org/https://doi.org/10.1007/978-3-030-52966-6_2
  • Yaman, C., Erenler, R., Atalar, M. N., Adem, Ş., & Çalişkan, U. K. (2024). Phytochemical Properties, Antioxidant and in Vitro/in Silico Anti-Acetylcholinesterase Activities of Hypericum heterophyllum Leaf from Türkiye. Brazilian Archives of Biology and Technology, 67, e24230043. https://doi.org/https://doi.org/10.1590/1678-4324-2024230043
  • Zhang, R., Ji, Y., Zhang, X., Kennelly, E. J., & Long, C. (2020). Ethnopharmacology of Hypericum species in China: A comprehensive review on ethnobotany, phytochemistry and pharmacology. Journal of ethnopharmacology, 254, 112686. https://doi.org/https://doi.org/10.1016/j.jep.2020.112686
  • Zhao, J., Davis, L. C., & Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology advances, 23(4), 283-333. https://doi.org/https://doi.org/10.1016/ j.biotechadv. 2005.01.003
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Bitki Biyoteknolojisi
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Şeyma Önlü 0000-0003-2005-1019

Proje Numarası 18-FEF-4901-02
Erken Görünüm Tarihi 10 Haziran 2025
Yayımlanma Tarihi
Gönderilme Tarihi 6 Ocak 2025
Kabul Tarihi 17 Mayıs 2025
Yayımlandığı Sayı Yıl 2025Cilt: 28 Sayı: 4

Kaynak Göster

APA Önlü, Ş. (2025). Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 1083-1094. https://doi.org/10.18016/ksutarimdoga.vi.1614168
 Kapak Resmi İndir

21082



2022-JIF = 0.500

2022-JCI = 0.170

Uluslararası Hakemli Dergi (International Peer Reviewed Journal)

       Dergimiz, herhangi bir başvuru veya yayımlama ücreti almamaktadır. (Free submission and publication)

      Yılda 6 sayı yayınlanır. (Published 6 times a year)


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