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Tuz Stresinin Fizyolojik Özellikler, Malondialdehit ve Prolin Birikimi Üzerindeki Etkileri ve Soya Fasulyesi [Glycine Max (L) Merrill]'Nin Ozmotik Ayarlamasi Ile İlişkisi

Year 2026, Volume: 29 Issue: 1, 79 - 97

Büşra Küçükkılııç , Öner Canavar , Hatice Kübra Gören

https://doi.org/10.18016/ksutarimdoga.vi.1699900

Abstract

Bu çalışma, on adet soya (Glycine max L. Merrill) çeşidinin fide döneminden V4 (üçüncü üç yaprak düğümü) büyüme evresine kadar, üç farklı tuz konsantrasyonuna (0, 3 ve 6 dS m⁻¹) karşı morfolojik, fizyolojik ve biyokimyasal tepkilerini değerlendirmiştir. Artan tuz düzeyleri; bitki boyu, yaş ve kuru ağırlık, yaprak alanı, göreceli su içeriği, klorofil a, klorofil b, SPAD ve karotenoid miktarlarında anlamlı azalmaya yol açarken; göreceli membran geçirgenliği (RMP), prolin ve malondialdehit (MDA) düzeylerinde artışa neden olmuştur. Bu çalışma, tuz stresi ile göreceli membran geçirgenliği (RMP) arasında 0.821 düzeyinde pozitif bir korelasyon olduğunu ortaya koymuştur. Ayrıca, tüm soya çeşitlerinde artan yaprak geçirgenliği, oksidatif zarara bağlı olarak diğer ölçülen özelliklerle anlamlı düzeyde negatif korelasyonlar göstermiştir.Özellikle yüksek MDA ve düşük prolin içeriğine sahip çeşitlerin tuz stresine karşı daha az kuru madde ürettiği görülmüş; bu da oksidatif zararın ve sınırlı osmotik koruyucu birikiminin etkisini ortaya koymuştur. Bulgular, prolin birikiminin tuza dayanıklı genotiplerin belirlenmesinde ve tuzlu koşullarda ürün dayanımının artırılmasında önemli bir gösterge olduğunu göstermektedir. Ayrıca, prolin ve MDA düzeylerinin çeşitler arasında farklılık göstermesi, tuz stresine karşı genotip özgü savunma mekanizmalarının varlığını ortaya koymuştur.

Keywords

Oksidatif stres Fizyolojik reaksiyonlar Solüt birikimi Tuz stresi S oya fasulyesi

Project Number

ZRF-21047

References

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Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]

Year 2026, Volume: 29 Issue: 1, 79 - 97

Büşra Küçükkılııç , Öner Canavar , Hatice Kübra Gören

https://doi.org/10.18016/ksutarimdoga.vi.1699900

Abstract

This study evaluated the morphological, physiological, and biochemical responses of ten soybean (Glycine max L. Merrill) cultivars to three levels of salt stress (0, 3, and 6 dS m⁻¹) from seedling emergence to the V4 growth stage. Increasing salt concentrations significantly reduced plant height, fresh and dry weights, leaf area, relative water content, chlorophyll a, chlorophyll b, SPAD, and carotenoid levels. In contrast, relative membrane permeability (RMP), proline, and malondialdehyde (MDA) levels increased with salt stress. This study showed that there was a 0.821** positive correlation between salt stress and rmp with the increasing salt stress, besides the increasing leaf permeability significantly in the leaves of all cultivars had a negatively significant correlation relationship with other measured traits, because of oxidative membrane damage. Notably, cultivars with higher MDA and lower proline contents produced less biomass under stress, emphasizing the role of oxidative damage and limited osmoprotectant accumulation. These findings underline proline accumulation as a key indicator for selecting salt-tolerant genotypes and improving crop resilience in saline environments. Additionally, the variation in proline and MDA responses among cultivars suggests genotype-specific mechanisms of salt stress tolerance.

Keywords

Oxidative stress Physiological reactions Solute accumulation Salt stress Soybean

Project Number

ZRF-21047

References

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  • Antoniou, C., Savvides, A., Christou, A., & Fotopoulos, V. (2016). Unravelling chemical priming machinery in plants: The role of reactive oxygen–nitrogen–sulfur species in abiotic stress tolerance enhancement. Current Opinion in Plant Biology, 33(1), 101–107. https://doi.org/10.1016/j.pbi.2016.06.020.
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  • Barberon, M. (2017). The Endodermis as a checkpoint for nutrients. New Phytologist, 213(4), 1604–1610. https://doi.org/10.1111/nph.14140.
  • Bates, L., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205–207. https://doi.org/10.1007/BF00018060.
  • Bharath, P., Gahir, S., & Raghavendra, A. S. (2021). Abscisic acid-induced stomatal closure: An important component of plant defense against abiotic and biotic stress. Frontiers in Plant Science, 12(1), 615114. https://doi.org/10.3389/fpls.2021.615114.
  • Bryant, C., Fuenzalida, T. I., Brothers, N., Mencuccini, M., Sack, L., Binks, O., & Ball, M. C. (2021). Shifting access to pools of shoot water sustains gas exchange and increases stem hydraulic safety during seasonal atmospheric drought. Plant Cell and Environment, 44(11), 2898–2911. https://doi.org/10.1111/pce.14080.
  • Burssens, S., Himanen, K., Van de Cotte, B., Beeckman, T., Van Montagu, M., Inzé, D., & Verbruggen, N. (2000). Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana. Planta, 211(5), 632–640. https://doi.org/10.1007/s004250000334.
  • Canavar, Ö., Götz, K. P., Ellmer, F., Chmielewski, F. M., & Kaynak, M. A. (2014). Determination of the relationship between water use efficiency, carbon isotope discrimination and proline in sunflower genotypes under drought stress. Australian Journal of Crop Science, 8(2), 232–242. https://search.informit.org/doi/10.3316/ informit.198767721017085.
  • Canavar, Ö., Gören, H. K., Tan, U., Yilmaz, O., Kaptan, M. A., & Küçük Kaya, S. (2025). Physiological responses of sunflower (Helianthus annuus L.) to multiple combined prolonged drought stress, salinity stress and boron toxicity: insights from pre‐and post‐recovery stages. Journal of Agronomy and Crop Science, 211(2), e70047. https://doi.org/10.1111/jac.70047
  • Conn, S., & Gilliham, M. (2010). Comparative physiology of elemental distributions in plants. Annals of Botany, 105(7), 1081–1102. https://doi.org/10.1093/aob/mcq027
  • Doğan, R., Şahin, U., & Kızılgeçi, F. (2015). Tarımsal üretimde soya fasulyesinin yeri ve önemi. Tarım Bilimleri Dergisi, 21(2), 123–132.
  • Doğru, A., & Canavar, S. (2020). Bitkilerde tuz toleransının fizyolojik ve biyokimyasal bileşenleri. Academic Platform Journal of Engineering and Science, 8(1), 155–174. https://doi.org/10.21541/apjes.541620
  • Dos Santos, T. B., Budzinski, I. G., Marur, C. J., Petkowicz, C. L., Pereira, L. F., & Vieira, L. G. (2011). Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and stachyose in response to abiotic stresses. Plant Physiology and Biochemistry, 49(4), 441–448. https://doi.org/10.1016/ j.plaphy.2011.01.023
  • Düzgüneş, O., & Akman, H. (1985). Varyasyon Kaynakları. A.Ü. Ziraat Fakültesi Yayınları, 954, 151.
  • Düzgüneş, O., Kesici, T., Kavuncu, O., & Gürbüz, F. (1987). Araştırma ve Deneme Metodları. A.Ü. Ziraat Fakültesi Yayınları, 1021, 1–381.
  • Flam-Shepherd, R., Huynh, W. Q., Coskun, D., Hamam, A. M., Britto, D. T., & Kronzucker, H. J. (2018). Membrane fluxes, bypass flows, and sodium stress in rice: The influence of silicon. Journal of Experimental Botany, 69(7), 1679–1692. https://doi.org/10.1093/jxb/erx460.
  • Frechilla, S., Lasa, B., Ibarretxe, L., Lamsfus, C., & Aparicio-Tejo, P. (2001). Pea responses to saline stress is affected by the source of nitrogen nutrition (Ammonium or Nitrate). Plant Growth Regulation, 35(2), 171–179. https://doi.org/10.1023/A:1014487908495.
  • Fricke, W., Akhiyarova, G., Wei, W., Alexandersson, E., Miller, A., Kjellbom, P. O., Richardson, A., Wojciechowski, T., Schreiber, L., Veselov, D., Kudoyarova, G., & Volkov, V. (2006). The short-term growth response to salt of the developing barley leaf. Journal of Experimental Botany, 57(5), 1079–1095.
  • Glenn, E. P., Brown, J. J., & Khan, M. J. (1997). Mechanisms of salt tolerance in higher plants. In: Basra, A. S., & Basra, R. K. (Eds.), Mechanisms of environmental stress resistance in plants (pp. 83–110). Harwood Academic Publishers.
  • Gong, Z. (2021). Plant abiotic stress: New insights into the factors that activate and modulate plant responses. Journal of Integrative Plant Biology, 63(3), 429–430. https://onlinelibrary.wiley.com/doi/10.1111/jipb.13079
  • Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J. & Ahmad, A. (2012). Role of proline under changing environments: A review. Plant Signaling & Behavior 7(11), 1456–1466. https://doi.org/10.4161/psb.21949.
  • Hoagland, D.R., & Arnon, D.I. (1950). The water-culture method for growing plants without soil. Circular. California Agricultural Experiment Station, 347, 32.
  • Hodges, D., DeLong, J., Forney, C. & Prange, R. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207(4), 604–611. https://doi.org/10.1007/s004250050524.
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There are 57 citations in total.

Details

Primary Language English
Subjects Agronomy
Journal Section RESEARCH ARTICLE
Authors

Büşra Küçükkılııç 0000-0002-1479-7909

Öner Canavar 0000-0003-4168-953X

Hatice Kübra Gören 0000-0001-7654-1450

Project Number ZRF-21047
Early Pub Date October 18, 2025
Publication Date October 19, 2025
Submission Date May 15, 2025
Acceptance Date August 15, 2025
Published in Issue Year 2026 Volume: 29 Issue: 1

Cite

APA Küçükkılııç, B., Canavar, Ö., & Gören, H. K. (2025). Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(1), 79-97. https://doi.org/10.18016/ksutarimdoga.vi.1699900
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