In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey

Serhat Keser; Fatma Keser; İsmail Turkoglu; Omer Kaygılı; Suat Tekın; Ersin Demır; Mustafa Karatepe; Okkes Yılmaz; Sevda Kırbag; Suleyman Sandal; Semra Turkoglu

doi:10.18016/ksutarimdoga.vi.589279

Araştırma Makalesi

Doğu Anadolu, Türkiye’de Yetişen Üç Centaurea L. Türünün in vitro Biyolojik Değerlendirilmesi ve Fitokimyasal Özellikleri

Yıl 2020, Cilt: 23 Sayı: 1, 148 - 156, 28.02.2020

Serhat Keser , Fatma Keser İsmail Turkoglu , Omer Kaygılı , Suat Tekın Ersin Demır , Mustafa Karatepe , Okkes Yılmaz , Sevda Kırbag , Suleyman Sandal , Semra Turkoglu

https://doi.org/10.18016/ksutarimdoga.vi.589279

Cited By: 8

Öz

Centaurea
türleri halk arasında tıbbi bitkiler olarak soğuk algınlığı, apse, peptik
ülser, hemoroit, diyabet vb. hastalıkların tedavisinde kullanılmaktadırlar.
Sunulan çalışmada, üç Centaurea türünün
antiradikal özellikleri, fitokimyasal içerikleri, antimikrobiyal ve antiproliferatif
aktiviteleri incelenmiştir.
Centaurea saligna (K.Koch) Wagenitz metanol
(99.94%), Centaurea virgata Lam. metanol (98.23%) ve su
(98.10%) ekstraktları standart antioksidan trolokstan (96.79%) daha yüksek ABTS
yok etme aktivitesi göstermiştir. Centaurea
kurdica Reichardt ekstraktları trolokstan daha düşük antiradikal aktivite
göstermiştir. Centaurea ekstraktları
bazı mikroorganizmalara karşı antimikrobiyal aktivite göstermiştir. Centaurea türlerinin yüksek miktarda toplam flavonoit,
fenolik ve proantosiyanidin, fenolik asitler, fitosteroller ve doymamış yağ
asitleri içerdiği belirlenmiştir. Ayrıca bu üç Centaurea ekstraktları MCF-7, HCT-116 ve LNCaP kanser hücre
serileri üzerinde çok yüksek antiproliferatif özellik göstermiştir.

Anahtar Kelimeler

Centaurea , Endemik , Antiradikal , Fitokimyasal , Antiproliferatif

Proje Numarası

114Z124

Kaynakça

Aktumsek A, Guler GO, Cakmak YS, Duran A, 2013a. Assessment of the antioxidant potential and fatty acid composition of four Centaurea L. taxa from Turkey. Food Chem, 141: 91–97.
Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A, 2011. Screening for in vitro antioxidant properties and fatty acid profiles of five Centaurea L. species from Turkey flora. Food Chem Toxicol, 49: 2914–2920.
Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A, 2013b. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem Toxicol, 55: 290–296.
Amaeze OU, Ayoola GA, Sofidiya MO, Adepoju-Bello AA, Adegoke AO, Coker HAB, 2011. Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves. Oxid Med Cell Longev, Article ID 976701, 7 pages.
Arif R, Küpeli E, Ergun F, 2004. The biological activity of Centaurea L. species. Gazi Univ J Sci, 17: 149–164.
Brand-Williams W, Cuvelier ME, Berset C, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol, 28: 25–30.
Bruno M, Modica A, Catinella G, Canli C, Arasoglu T, Celik S, 2018. Chemical composition of the essential oils of Centaurea tomentella Hand.-Mazz. and C. haussknechtii Boiss. (Asteraceae) collected wild in Turkey and their activity on microorganisms affecting historical art craft. Nat Prod Res, Accepted Manuscript DOI:10.1080/14786419.2018.1463531.
Christie WW, 1992. Gas chromatography and lipids. The Oil Press, Glasgow.
Collins CM, Lyne PM, 1989. Microbiological Methods, Buttermorths-Heinemann, London, England.
Davis PH, 1988. Flora of Turkey and the East Aegean Islands (Vol. 10). Edinburgh, Edinburgh University Press.
Denizot F, Lang R, 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods, 89: 271–277.
Dittrich M, 1977. Cinareae-systematic review. In: Heywood, V.H., Harborne, J.B., Turner, B.L. (Eds.), The Biology and Chemistry of the Compositae. Academic Press, London, New York, San Francisco, pp. 999–1015.
Formisano C, Rigano D, Senatore F, Celik S, Bruno M, Rosselli S, 2008. Volatile constituents of aerial parts of three endemic Centaurea species from Turkey: Centaurea amanicola Hub.-Mor., Centaurea consanguinea DC. and Centaurea ptosimopappa Hayek and their antibacterial activities. Nat Prod Res, 22: 833–839.
Güven K, Çelik S, Uysal İ, 2005. Antimicrobial activity of Centaurea species. Pharmaceut Biol, 43: 67–71.Halliwell B, Gutteridge JMC, Aruoma O, 1987. The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem, 165: 215–219.
Iwu MW, Duncan AR, Okunji CO, 1999. New antimicrobials of plant origin. In: Janick J., eds. Perspectives on new crops and new uses. ASHS Press, Alexandria, VA.
Keser S, 2014. Antiradical activities and phytochemical compounds of firethorn (Pyracantha coccinea) fruit extracts. Nat Prod Res, 28: 1789–1794.
Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY, 2003. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agr Food Chem, 51: 6509–6515.
López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ, 2006. High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. J Chromatogr A, 1105: 135–139.
Modi C, Mody S, Patel H, Dudhatra G, Kumar A, Awale M, 2012. Herbal antibacterials: a review. J Intercult Ethnopharmacol, 1: 52–61.
Mosmann T, 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55–63.
Pandey G, Madhuri S, 2009. Some medicinal plants as natural anticancer agents. Pharmacogn Rev, 3: 259–263.
Prema R, Sekar SD, Sekhar KBC, 2011. Review on: Herbs as anticancer agents. Int J Pharmacy Indust Res, 1: 105–108.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C, 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med, 26: 1231–1237.
Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P, 2002. High performance liquid chromatographic determination of alpha-tocopherol in macroalgae. J Chromatogr A, 976: 277–284.
Sarker SD, Nahar L, Guija S, Begum S, Celik S, 2012. Bioactivity of Centaurea persica Boiss. (Asteraceae). Arch Biol Sci, 64: 517–523.
Slinkard K, Singleton VL, 1977. Total phenol analysis-automation and comparison with manual methods. Am J Enol Viticult, 28: 49–55.
Tekeli Y, Sezgin M, Aktumsek A, 2008. Antioxidant property of Centaurea solstitialis L. from Konya, Turkey. Asian J Chem, 20: 4831–4835.
Tekeli Y, Sezgin M, Aktumsek A, Guler GO, Sanda MA, 2010. Fatty acid composition of six Centaurea species growing in Konya, Turkey. Nat Prod Res, 24: 1883–1889.
Ugur A, Duru ME, Ceylan O, Sarac N, Varol O, Kivrak I, 2009. Chemical composition, antimicrobial and antioxidant activities of Centaurea ensiformis Hub.-Mor. (Asteraceae), a species endemic to Mugla (Turkey). Nat Prod Res, 23: 149–167.
Uysal I, Celik S, Saglam H, Güven K, 2013. Antimicrobial and antioxidant activities of some species of Centaurea collected from Turkey. Asian J Chem, 25: 666–670.
Wagenitz G, Hellwig FH, 1996. Evolution of characters and phylogeny of Centaureinea. In: Hinf, D.J.N., Beentje, H.J. (Eds.), Compositae: Systematics. Proceedings of the International Compositae Conference, Kew, 1994, vol. 1. Royal Botanic Gardens, Kew, UK, pp. 491–510.
Wen T, Jinjian L, Mingqing H, Yingbo Li, Meiwan C, Guosheng W, Jian G, Zhangfeng Z, Zengtao X, Yuanye, D, Jiajie G, Xiuping C, Yitao W, 2011. Anti-cancer natural products isolated from Chinese medicinal herbs. Chinese Med, 6: 1–15.
Zengin G, Aktumsek A, Guler GO, Cakmak YS, Kan Y, 2012. Composition of essential oil and antioxidant capacity of Centaurea drabifolia Sm. subsp. detonsa (Bornm.) Wagenitz, endemic to Turkey. Nat Prod Res, 26: 1–10.
Zengin G, Cakmak YS, Guler GO, Aktumsek A, 2010. In vitro antioxidant capacities and fatty acid compositions of three Centaurea species collected from Central Anatolia region of Turkey. Food Chem Toxicol, 48: 2638–2641.
Zu YG, Li CY, Fu YJ, Zhao CJ, 2006. Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD. J Pharmaceut Biomed, 41: 714–719.

In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey

Yıl 2020, Cilt: 23 Sayı: 1, 148 - 156, 28.02.2020

Serhat Keser , Fatma Keser İsmail Turkoglu , Omer Kaygılı , Suat Tekın Ersin Demır , Mustafa Karatepe , Okkes Yılmaz , Sevda Kırbag , Suleyman Sandal , Semra Turkoglu

https://doi.org/10.18016/ksutarimdoga.vi.589279

Cited By: 8

Öz

Centaurea L.
species were used as medicinal plants among the people for treatment of the
common cold, abscesses, peptic ulcers, hemorrhoid and diabetes etc.. In the
present study, antiradical properties, phytochemical contents, antimicrobial
and antiproliferative activities of three Centaurea
species
were investigated. Centaurea
saligna (K.Koch) Wagenitz methanol
(99.94%), Centaurea virgata Lam. methanol (98.23%) and water
(98.10%) extracts were showed higher ABTS scavenging than trolox (96.79%). Centaurea kurdica Reichardt extracts
showed lower activity than trolox for all the antiradical assays. Centaurea
extracts exhibited antimicrobial activity against to some microorganisms. It
was determined that these Centaurea
species contain high amount of total flavonoid, phenolic and proanthocyanidin,
phenolic acids, phytosterols and unsaturated fatty acids. Also, three Centaurea extracts showed very high antiproliferative
property on LNCaP, HCT-116, MCF-7 cancer cell lines.

Anahtar Kelimeler

Centaurea , Endemic , Antiradical , Phytochemical , Antiproliferative

Destekleyen Kurum

TÜBİTAK

Proje Numarası

114Z124

Teşekkür

This study was supported by TUBITAK, under grant number 114Z124.

Kaynakça

Aktumsek A, Guler GO, Cakmak YS, Duran A, 2013a. Assessment of the antioxidant potential and fatty acid composition of four Centaurea L. taxa from Turkey. Food Chem, 141: 91–97.
Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A, 2011. Screening for in vitro antioxidant properties and fatty acid profiles of five Centaurea L. species from Turkey flora. Food Chem Toxicol, 49: 2914–2920.
Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A, 2013b. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem Toxicol, 55: 290–296.
Amaeze OU, Ayoola GA, Sofidiya MO, Adepoju-Bello AA, Adegoke AO, Coker HAB, 2011. Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves. Oxid Med Cell Longev, Article ID 976701, 7 pages.
Arif R, Küpeli E, Ergun F, 2004. The biological activity of Centaurea L. species. Gazi Univ J Sci, 17: 149–164.
Brand-Williams W, Cuvelier ME, Berset C, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol, 28: 25–30.
Bruno M, Modica A, Catinella G, Canli C, Arasoglu T, Celik S, 2018. Chemical composition of the essential oils of Centaurea tomentella Hand.-Mazz. and C. haussknechtii Boiss. (Asteraceae) collected wild in Turkey and their activity on microorganisms affecting historical art craft. Nat Prod Res, Accepted Manuscript DOI:10.1080/14786419.2018.1463531.
Christie WW, 1992. Gas chromatography and lipids. The Oil Press, Glasgow.
Collins CM, Lyne PM, 1989. Microbiological Methods, Buttermorths-Heinemann, London, England.
Davis PH, 1988. Flora of Turkey and the East Aegean Islands (Vol. 10). Edinburgh, Edinburgh University Press.
Denizot F, Lang R, 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods, 89: 271–277.
Dittrich M, 1977. Cinareae-systematic review. In: Heywood, V.H., Harborne, J.B., Turner, B.L. (Eds.), The Biology and Chemistry of the Compositae. Academic Press, London, New York, San Francisco, pp. 999–1015.
Formisano C, Rigano D, Senatore F, Celik S, Bruno M, Rosselli S, 2008. Volatile constituents of aerial parts of three endemic Centaurea species from Turkey: Centaurea amanicola Hub.-Mor., Centaurea consanguinea DC. and Centaurea ptosimopappa Hayek and their antibacterial activities. Nat Prod Res, 22: 833–839.
Güven K, Çelik S, Uysal İ, 2005. Antimicrobial activity of Centaurea species. Pharmaceut Biol, 43: 67–71.Halliwell B, Gutteridge JMC, Aruoma O, 1987. The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem, 165: 215–219.
Iwu MW, Duncan AR, Okunji CO, 1999. New antimicrobials of plant origin. In: Janick J., eds. Perspectives on new crops and new uses. ASHS Press, Alexandria, VA.
Keser S, 2014. Antiradical activities and phytochemical compounds of firethorn (Pyracantha coccinea) fruit extracts. Nat Prod Res, 28: 1789–1794.
Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY, 2003. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agr Food Chem, 51: 6509–6515.
López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ, 2006. High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate. J Chromatogr A, 1105: 135–139.
Modi C, Mody S, Patel H, Dudhatra G, Kumar A, Awale M, 2012. Herbal antibacterials: a review. J Intercult Ethnopharmacol, 1: 52–61.
Mosmann T, 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods, 65: 55–63.
Pandey G, Madhuri S, 2009. Some medicinal plants as natural anticancer agents. Pharmacogn Rev, 3: 259–263.
Prema R, Sekar SD, Sekhar KBC, 2011. Review on: Herbs as anticancer agents. Int J Pharmacy Indust Res, 1: 105–108.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C, 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med, 26: 1231–1237.
Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losado P, 2002. High performance liquid chromatographic determination of alpha-tocopherol in macroalgae. J Chromatogr A, 976: 277–284.
Sarker SD, Nahar L, Guija S, Begum S, Celik S, 2012. Bioactivity of Centaurea persica Boiss. (Asteraceae). Arch Biol Sci, 64: 517–523.
Slinkard K, Singleton VL, 1977. Total phenol analysis-automation and comparison with manual methods. Am J Enol Viticult, 28: 49–55.
Tekeli Y, Sezgin M, Aktumsek A, 2008. Antioxidant property of Centaurea solstitialis L. from Konya, Turkey. Asian J Chem, 20: 4831–4835.
Tekeli Y, Sezgin M, Aktumsek A, Guler GO, Sanda MA, 2010. Fatty acid composition of six Centaurea species growing in Konya, Turkey. Nat Prod Res, 24: 1883–1889.
Ugur A, Duru ME, Ceylan O, Sarac N, Varol O, Kivrak I, 2009. Chemical composition, antimicrobial and antioxidant activities of Centaurea ensiformis Hub.-Mor. (Asteraceae), a species endemic to Mugla (Turkey). Nat Prod Res, 23: 149–167.
Uysal I, Celik S, Saglam H, Güven K, 2013. Antimicrobial and antioxidant activities of some species of Centaurea collected from Turkey. Asian J Chem, 25: 666–670.
Wagenitz G, Hellwig FH, 1996. Evolution of characters and phylogeny of Centaureinea. In: Hinf, D.J.N., Beentje, H.J. (Eds.), Compositae: Systematics. Proceedings of the International Compositae Conference, Kew, 1994, vol. 1. Royal Botanic Gardens, Kew, UK, pp. 491–510.
Wen T, Jinjian L, Mingqing H, Yingbo Li, Meiwan C, Guosheng W, Jian G, Zhangfeng Z, Zengtao X, Yuanye, D, Jiajie G, Xiuping C, Yitao W, 2011. Anti-cancer natural products isolated from Chinese medicinal herbs. Chinese Med, 6: 1–15.
Zengin G, Aktumsek A, Guler GO, Cakmak YS, Kan Y, 2012. Composition of essential oil and antioxidant capacity of Centaurea drabifolia Sm. subsp. detonsa (Bornm.) Wagenitz, endemic to Turkey. Nat Prod Res, 26: 1–10.
Zengin G, Cakmak YS, Guler GO, Aktumsek A, 2010. In vitro antioxidant capacities and fatty acid compositions of three Centaurea species collected from Central Anatolia region of Turkey. Food Chem Toxicol, 48: 2638–2641.
Zu YG, Li CY, Fu YJ, Zhao CJ, 2006. Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD. J Pharmaceut Biomed, 41: 714–719.

Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Yapısal Biyoloji
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Serhat Keser 0000-0002-9678-1053 Fatma Keser 0000-0001-6870-0546 İsmail Turkoglu 0000-0001-7454-7605 Omer Kaygılı 0000-0002-2321-1455 Suat Tekın 0000-0002-2757-1802 Ersin Demır 0000-0002-7676-5953 Mustafa Karatepe 0000-0001-6358-5913 Okkes Yılmaz 0000-0002-8276-4498 Sevda Kırbag 0000-0002-4337-8236 Suleyman Sandal 0000-0002-8916-3329 Semra Turkoglu 0000-0001-7682-0513
Proje Numarası	114Z124
Yayımlanma Tarihi	28 Şubat 2020
Gönderilme Tarihi	9 Temmuz 2019
Kabul Tarihi	26 Eylül 2019
Yayımlandığı Sayı	Yıl 2020 Cilt: 23 Sayı: 1

Kaynak Göster

APA	Keser, S., Keser, F., Turkoglu, İ., … Kaygılı, O. (2020). In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(1), 148-156. https://doi.org/10.18016/ksutarimdoga.vi.589279