Investigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye

Enis Fuat Tüfekci; Ümit Uzun; Nagihan Sağlam Ertunga; Ayşenur Biber; İnanç Alptuğ Hıdıroğlu; İlayda Tekkılıç; Birhan Altay; Ali Osman Kılıç

doi:10.18016/ksutarimdoga.vi.1226184

EN TR

Investigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye

Abstract

Considering that most antibiotics originate from actinomycete group bacteria, especially the Streptomyces genus, it is predicted that novel actinomycetes isolated from extreme environments such as caves may bring novel antibiotics to the medical world. The study aimed to screen the antimicrobial activity of actinomycetes isolated from the three karst caves in Türkiye and to identify selected isolates with antimicrobial activity by molecular methods. One hundred seventy-nine actinomycetes isolated from Akçakale, Kırklar (Altıntaş), and Köprübaşı Caves in Gümüşhane province in the Eastern Black Sea Region of Türkiye were included in the study. The antimicrobial activity of isolates was investigated using the modified cross-streak agar method against seven Gram-negative bacteria, three Gram-positive bacteria, and one yeast strain. Fifty-three isolates (29.6%) had antimicrobial activity against at least one of the tested microorganisms. The rate of isolates exhibiting antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Chromobacterium violaceum, Klebsiella pneumoniae, Salmonella Typhimurium, Escherichia coli, Acinetobacter haemolyticus, Pseudomonas aeruginosa, Enterobacter aerogenes, and Candida albicans was 21.2%, 20.0%, 16.8%, 12.8%, 3.4%, 2.8%, 2.2%, 1.1%, 0.6%, 0.6%, and 0.6%, respectively. An actinomycete isolate, TRMS 124, showed antimicrobial activity against ten test microorganisms. The 16S ribosomal RNA (16S rRNA) sequencing was performed for the identification and phylogenetic analysis of 26 isolates randomly selected among actinomycetes that exhibited antimicrobial activity against at least three test microorganisms. As a result, it was determined that 24 isolates showed homology with various Streptomyces species and two isolates with Embleya scabrispora and Couchioplanes caeruleus, respectively. These results showed that karst caves could be good sources for isolating actinomycetes with the potential to produce antimicrobial compounds.

Keywords

Doğu Karadeniz Bölgesindeki Karstik Mağaralardan İzole Edilen Aktinomisetlerin Antimikrobiyal Aktivitelerinin ve 16S rRNA Dizilerinin Araştırılması

Öz

Günümüzde kullanılan antibiyotiklerin büyük çoğunluğunun başta Streptomyces cinsi olmak üzere çeşitli aktinomiset grubu bakterilerden orijin aldığı düşünüldüğünde, mağara gibi ekstrem ortamlardan izole edilecek yeni aktinomisetlerin tıp dünyasına yeni antibiyotikler kazandırabileceği öngörülmektedir. Bu çalışmada, üç farklı karstik mağaradan izole edilen aktinomiset izolatlarının antimikrobiyal aktivitelerinin araştırılması ve etkili izolatların moleküler yöntemlerle tanımlanması amaçlanmıştır. Çalışmaya Türkiye’nin Doğu Karadeniz Bölgesinde bulunan Gümüşhane ilindeki Akçakale, Kırklar (Altıntaş) ve Köprübaşı mağaralarından izole edilen 179 aktinomiset izolatı dahil edilmiştir. İzolatların antimikrobiyal aktiviteleri yedi Gram-negatif, üç Gram-pozitif ve bir maya suşuna karşı çapraz çizgi yöntemi ile araştırılmıştır. Elli üç izolatın (%29,6) test edilen mikroorganizmalardan en az birine karşı antimikrobiyal aktiviteye sahip olduğu bulunmuştur. İzolatların Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Chromobacterium violaceum, Klebsiella pneumoniae, Salmonella Typhimurium, Escherichia coli, Acinetobacter haemolyticus, Pseudomonas aeruginosa, Enterobacter aerogenes ve Candida albicans’a karşı antimikrobiyal aktivite sergileme oranı sırasıyla %21.2 %20.0, %16.8, %12.8, %3.4, %2.8, %2.2, %1.1, %0.6, %0.6, %0.6 şeklinde bulunmuştur. TRMS 124 olarak adlandırılan bir aktinomiset izolatı, 10 test mikroorganizmasına karşı antimikrobiyal aktivite sergilemiştir. En az üç test mikroorganizmasına karşı antimikrobiyal aktivite sergileyen aktinomisetler arasından randomize olarak seçilen 26 izolatın tanımlanması ve filogenetik analizi için 16S ribozomal RNA (16S rRNA) dizi analizi yapılmıştır. Buna göre 24 izolatın çeşitli Streptomyces türleri ile iki izolatın ise sırasıyla Embleya scabrispora ve Couchioplanes caeruleus ile homoloji gösterdiği tespit edilmiştir. Bu çalışmadan elde edilen bulgular karstik mağaraların antimikrobiyal madde üretme potansiyeline sahip aktinomisetlerin izolasyonu için doğal kaynaklar olabileceğini göstermiştir.

Anahtar Kelimeler

Destekleyen Kurum

Karadeniz Teknik Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

FBA-2016-5481

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Mikrobiyoloji (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Enis Fuat Tüfekci ^*
0000-0001-5051-2694
Türkiye

Ümit Uzun
0000-0002-0585-2865
Türkiye

Nagihan Sağlam Ertunga
0000-0002-2104-2192
Türkiye

Ayşenur Biber
0000-0001-8431-2703
Türkiye

İnanç Alptuğ Hıdıroğlu
0000-0002-3030-6006
Türkiye

İlayda Tekkılıç
0000-0002-2522-7370
Türkiye

Birhan Altay
0000-0003-2025-7869
Türkiye

Ali Osman Kılıç
0000-0002-5506-0866
Türkiye

Erken Görünüm Tarihi

14 Haziran 2023

Yayımlanma Tarihi

31 Aralık 2023

Gönderilme Tarihi

31 Aralık 2022

Kabul Tarihi

8 Mayıs 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 26 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1226184

IZ

https://izlik.org/JA82PA32HE

Kaynak Göster

RIS / Bibtex

APA

Tüfekci, E. F., Uzun, Ü., Sağlam Ertunga, N., Biber, A., Hıdıroğlu, İ. A., Tekkılıç, İ., Altay, B., & Kılıç, A. O. (2023). Investigation of Antimicrobial Activities and 16S rRNA Sequences of Actinomycetes Isolated from Karst Caves in the Eastern Black Sea Region of Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(6), 1277-1290. https://doi.org/10.18016/ksutarimdoga.vi.1226184