Evaluation of Biological Activities of Various Extracts of Glaucium alakirensis, Marrubium bourgaei, and Peucedanum alpinum from Türkiye

Eda Delik; Berfin Eroğlu; Mertcan Gülben; Candan Aykurt; Burcu Emine Tefon Öztürk

doi:10.18016/ksutarimdoga.vi.1388926

EN TR

Evaluation of Biological Activities of Various Extracts of Glaucium alakirensis, Marrubium bourgaei, and Peucedanum alpinum from Türkiye

Abstract

Plant species contain many secondary metabolites, and these compounds differ from species to species. These differences in the concentrations of these compounds have many health implications. Today, studies on plants' antioxidant and antibacterial effects are gaining importance. In particular, the adverse effects of some existing antibiotics and the constant development of bacterial resistance are leading to the search for new natural antimicrobial agents. In this study, methanol, ethanol, ethyl acetate, acetone, and chloroform extracts were obtained from the aerial parts of Marrubium bourgaei Boiss and Glaucium alakirensis Aykurt, K.Yıldız & A.Özçandır, and Peucedanum alpinum B.L.Burtt & Davis, species which are naturally distributed in Türkiye. The antioxidant activity of the extracts was determined by the DPPH (2,2 Difenil-1-pikrihidrazil) and ABTS (2,2' azino-bis(3-ethylbenz-thiazoline-6-sulfonic-acid)) methods, the total phenolic content by Folin-Ciocalteu method, the total flavonoid content by aluminium chloride colorimetric method, and the antibacterial activity against ten bacteria by the disc diffusion method. According to the results, methanol, ethanol, and acetone extracts had higher antioxidant activity, total phenolic, and total flavonoid contents than other extracts. However, the total flavonoid content of M. bourgaei was higher in the ethyl acetate extract. When evaluated for their antibacterial activity, ethanol, chloroform, and ethyl acetate in P. alpinum, chloroform in M. bourgaei, and methanol, chloroform, and ethyl acetate in G. alakirensis extracts showed antibacterial activity against more bacteria than others. This is the first study to evaluate and compare the total phenolic and flavonoid content, and antioxidant and antibacterial activities of 5 different extracts of these plants.

Keywords

Türkiye'den Glaucium alakirensis, Marrubium bourgaei, and Peucedanum alpinum’un Çeşitli Ekstraktlarının Biyolojik Aktivitelerinin Değerlendirilmesi

Öz

Bitki türleri birçok ikincil metabolit içerir ve içermiş oldukları bu bileşikler türden türe farklılık göstermektedir. Bitkilerin ikincil metabolitlerinin konsantrasyonlarındaki bu farklılıkların sağlık üzerinde farklı etkileri vardır. Bitkilerin antioksidan ve antibakteriyel etkileri üzerine yapılan çalışmalar günümüzde yeniden önem kazanmaktadır. Özellikle mevcut bazı antibiyotiklerin olumsuz etkileri ve sürekli olarak artan bakteriyel direnç, yeni ve doğal antimikrobiyal ajan arayışına neden olmaktadır. Bu çalışmada, Türkiye'de doğal olarak yayılış gösteren Marrubium bourgaei Boiss, Glaucium alakirensis Aykurt, K.Yıldız & A.Özçandır ve Peucedanum alpinum B.L.Burtt & Davis türlerinin toprak üstü kısımlarından metanol, etanol, etil asetat, aseton ve kloroform kullanılarak ekstraktlar elde edilmiştir. Bu ekstraktların antioksidan aktiviteleri DPPH (2,2 Difenil-1-pikrihidrazil) ve ABTS (2,2' azino-bis(3-etilbenz-tiazolin-6-sülfonik-asit)) yöntemleriyle, toplam fenolik madde içerikleri Folin-Ciocalteu yöntemiyle, toplam flavonoid madde içerikleri ise alüminyum klorür kolorimetrik yöntemi ile belirlenmiştir. Ayrıca elde edilen bu bitkisel ekstraktların antibakteriyel aktiviteleri disk difüzyon yöntemi kullanılarak on bakteri için değerlendirilmiştir. Elde edilen sonuçlara göre, bitkilerin metanol, etanol ve aseton ekstraktları diğer ekstraktlarından daha yüksek antioksidan aktivite, toplam fenolik ve flavonoid madde içeriğine sahiptir. Ancak, M. bourgaei'nin toplam flavonoid madde içeriği etil asetat ekstraktında daha yüksek bulunmuştur. Ekstraktların antibakteriyel aktiviteleri değerlendirildiğinde, P. alpinum'da etanol, kloroform ve etil asetat, M. bourgaei'de kloroform, G. alakirensis'de ise metanol, kloroform ve etil asetat ekstraktları diğer ekstraktlara göre daha fazla bakteriye karşı etkilidir. Gerçekleştirilen bu çalışma, kullanılan bu üç bitkinin beş farklı ekstraktının toplam fenolik madde içeriğini, toplam flavonoid madde içeriğini, antioksidan aktivitelerini ve antibakteriyel aktivitelerini değerlendiren ve karşılaştıran ilk çalışmadır.

Anahtar Kelimeler

Etik Beyan

Bu makale etik kurul kararı gerektiren çalışmalar içermemektedir

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası , Mikrobiyoloji (Diğer) , Tıbbi ve Aromatik Bitkiler

Bölüm

Araştırma Makalesi

Yazarlar

Eda Delik
0000-0002-9047-2874
Türkiye

Berfin Eroğlu
0000-0002-9099-7603
Türkiye

Mertcan Gülben
0000-0002-0096-7161
Türkiye

Candan Aykurt
0000-0001-8700-3500
Türkiye

Burcu Emine Tefon Öztürk ^*
0000-0003-1690-9879
Türkiye

Erken Görünüm Tarihi

15 Ağustos 2024

Yayımlanma Tarihi

7 Kasım 2024

Gönderilme Tarihi

10 Kasım 2023

Kabul Tarihi

14 Mayıs 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1388926

IZ

https://izlik.org/JA25CT98AP

Kaynak Göster

RIS / Bibtex

APA

Delik, E., Eroğlu, B., Gülben, M., Aykurt, C., & Tefon Öztürk, B. E. (2024). Evaluation of Biological Activities of Various Extracts of Glaucium alakirensis, Marrubium bourgaei, and Peucedanum alpinum from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(6), 1249-1261. https://doi.org/10.18016/ksutarimdoga.vi.1388926