Investigation of Chemical Composition and Biological Activity of Salix aegyptiaca L. Roots

Maaroof Abdalrahman Rasul; Ali Göçeri; Shno Abdalqadır Sofi; Mehmet Hakkı Alma; Ekrem Kireçci; Mustafa Abdullah Yılmaz

doi:10.18016/ksutarimdoga.vi.1402758

EN TR

Investigation of Chemical Composition and Biological Activity of Salix aegyptiaca L. Roots

Abstract

The root of Salix aegyptiaca L. was extracted using their yield percentage, total condensed tannin concentration, antimicrobial, antioxidant activity and to determine chemical composition by LC-MS/MS. The root extraction was carried out together with water, ethanol and methanol. Accelerated solvent extraction (ASE), conventional extraction (CE), and microwave extraction (ME) were the extraction methods applied during the investigation. The LC-MS/MS methanol extract was used to detect phenolics. The antioxidant activities and total condensed tannin concentrations of root extracts have been done by UV-visible spectroscopy from 517 to 580 nm, severally. The disk diffusion method was used for antimicrobial activity. The maximum extraction yield (17.2%) was obtained in methanol by the ASE technique whereas, the conventional extraction technique obtained the minimum extraction efficiency (9.1%). By triplicate measurement, the total condensed tannin analysis result was found 35.14 mg/L. Using the ASE technique, the methanol extract was the maximum inhibitory zone (26 mm) against Candida albicans ATCC 10231. However, in water extract by conventional extraction, a minimum inhibitory zone (11 mm) was obtained against Staphylococcus aureus Cowan 1. The highest and lowest DPPH scavenging activity was determined in methanol (ASE) (98.8%) and ethanol (97.5%) extract respectively. The maximum amounts of quinic acid (63895 µg/g) were discovered using LC-MS/MS.

Keywords

Salix aegyptiaca L. Köklerinin Kimyasal Bileşimi ve Biyolojik Aktivitesinin Araştırılması

Öz

Salix aegyptiaca'nın kök ekstraktı, verim yüzdesi, toplam kondense tanen konsantrasyonu, antimikrobiyal, antioksidan aktivite ve LC-MS/MS kullanılarak kimyasal bileşiminin belirlenmesi için kullanılmıştır. Kök ekstraksiyonu su, etanol ve metanol ile gerçekleştirildi. Hızlandırılmış solvent ekstraksiyonu (ASE), konvansiyonel ekstraksiyon (CE) ve mikrodalga ekstraksiyonu (ME), araştırma sırasında uygulanan ekstraksiyon yöntemleriydi. Fenoliklerin LC-MS/MS ile tespit edilmesi için metanol ekstraktı kullanıldı. Kök ekstraktlarının antioksidan aktiviteleri ve toplam kondanse tanen miktarları UV-Vis spektrofotometre cihazında sırasıyla 517 nm ve 580 nm olarak ölçülmüştür. Antimikrobiyal aktivite için disk difüzyon yöntemi kullanıldı. Maksimum ekstraksiyon verimi (%17.2) metanolde ASE tekniği ile elde edilirken, minimum ekstraksiyon verimliliği (%9.1) konvansiyonel ekstraksiyon tekniği ile elde edildi. Üçlü ölçümle toplam kondense tanen analizi sonucu 35.14 mg/L bulundu. ASE tekniği kullanıldığında metanol ekstraktı Candida albicans ATCC 10231'e karşı maksimum inhibitör bölge (26 mm) olmuştur. Bununla birlikte, su ekstraktında geleneksel ekstrasitasyonla Staphylococcus aureus Cowan 1'e karşı minimum inhibitör bölge (11 mm) elde edilmiştir. En yüksek ve en düşük DPPH giderme aktivitesi sırasıyla metanol (ASE) (%98.8) ve etanol (%97.5) ekstraktında belirlendi. LC-MS/MS kullanılarak maksimum kinik asit miktarları (63895 µg/g) keşfedildi.

Anahtar Kelimeler

Destekleyen Kurum

Kahramanmaraş Sütçü İmam Üniversitesi

Proje Numarası

2014/2-60 YLS

Teşekkür

Bu çalışmayı destekleyen Kahramanmaraş Sütçü İmam Üniversitesine Teşekkürler ederim.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Maaroof Abdalrahman Rasul
0009-0003-9241-957X
Iraq

Ali Göçeri ^*
0000-0002-1218-6696
Türkiye

Shno Abdalqadır Sofi
0009-0001-5596-3141
Iraq

Mehmet Hakkı Alma
0000-0001-6323-7230
Türkiye

Ekrem Kireçci
0000-0001-9446-8584
Türkiye

Mustafa Abdullah Yılmaz
0000-0002-4090-7227
Türkiye

Erken Görünüm Tarihi

15 Ağustos 2024

Yayımlanma Tarihi

7 Kasım 2024

Gönderilme Tarihi

10 Aralık 2023

Kabul Tarihi

8 Şubat 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1402758

IZ

https://izlik.org/JA38UY35XZ

Kaynak Göster

RIS / Bibtex

APA

Rasul, M. A., Göçeri, A., Sofi, S. A., Alma, M. H., Kireçci, E., & Yılmaz, M. A. (2024). Investigation of Chemical Composition and Biological Activity of Salix aegyptiaca L. Roots. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(6), 1237-1248. https://doi.org/10.18016/ksutarimdoga.vi.1402758

Cited By

Comparative analysis of extraction techniques and biological activities of the root of Tribulus terrestris L. grown in Northern Iraq

Harran Tarım ve Gıda Bilimleri Dergisi

https://doi.org/10.29050/harranziraat.1657116

Investigation of Chemical Composition and Biological Activity of Salix aegyptiaca L. Roots

Abstract

Keywords

Salix aegyptiaca L. Köklerinin Kimyasal Bileşimi ve Biyolojik Aktivitesinin Araştırılması

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Comparative analysis of extraction techniques and biological activities of the root of Tribulus terrestris L. grown in Northern Iraq

e-ISSN: 2619-9149