Antioxidant Properties of Methanolic Extract of Sigesbeckia orientalis L. from Rize

Mehtap Atak; Zeynep Bakan; Eda Yılmaz Kutlu; Merve Hüner Yiğit; Nebahat Ejder

doi:10.18016/ksutarimdoga.vi.1491528

TR EN

Rize Yöresinde Yetişen Sigesbeckia orientalis L. Metanollü Ekstresinin Antioksidan Özellikleri

Öz

Sigesbeckiae orientalis Linnaeus, uzun yıllardır enflamatuvar hastalıkların tedavisinde kullanılan geleneksel bir bitkisel ilaçtır. Sigesbeckiae cinsine ait üç türü bulunmaktadır: Sigesbeckia. pubescens Markino (SP), Sigesbeckia glabrescens Makino (SG) ve Sigesbeckia orientalis L. (SO). S. orientalis doğu tıbbında romatizmal rahatsızlıkları hafifletmek, eklemlerin hareketliliğini iyileştirmek ve toksisiteye karşı koruma sağlamak için kullanılmıştır. Bu bitki Çin, Filipinler ve Vietnam gibi Asya ülkelerinde doğal olarak yetişmektedir. Türkiye’de özellikle Karadeniz Bölgesinde yetişen S. orientalis Sarıteçan olarak adlandırılmaktadır. Bu çalışmada, S. orientalis’in metanollü ekstresinin antioksidan kapasitesi toplam fenolik madde miktarı (TPMA) ve toplam flavonoid miktarı (TFA) belirlenerek, antioksidan aktivitesi Ferrik İndirgeyici Antioksidan Güç (FRAP), Cu (II) İyonu İndirgeyici Antioksidan Kapasite (CUPRAC) ve 2,2-difenil-1-pikrilhidrazil Serbest Radikalleri Temizleme Kapasitesi (DPPH) analizleri yapılarak değerlendirildi. S. orientalis ekstresinin toplam fenolik madde miktarı (TPMA) 18 ± 0.57 mgGAE/g kuru ağırlık olarak belirlenirken toplam flavonoid miktarı (TFA) ise 8.55±0.21 mg QE/g kuru ağırlık olarak belirlendi. DPPH (SC50) 380±12 µg/mL bulunurken CUPRAC ve FRAP aktiviteleri sırasıyla 74 ± 1.99 ve 19 ±1.38 mg TE/g kuru ağırlık bulundu. Bu sonuçlar, Rize İkizdere bölgesinde yetişen S. orientalis bitkisinin güçlü antioksidan özelliklere sahip olduğunu ortaya koymaktadır. Bu nedenle, S. orientalis bitkisi, geleneksel tıbbın ötesinde modern tamamlayıcı tıpta da araştırılmaya değer bir potansiyele sahip olabilir.

Anahtar Kelimeler

Antioxidant Properties of Methanolic Extract of Sigesbeckia orientalis L. from Rize

Abstract

Sigesbeckia orientalis Linnaeus is a traditional herbal medicine that has been used for many years to treat inflammatory diseases. The genus Sigesbeckia comprises three species: Sigesbeckia pubescens Markino (SP), Sigesbeckia glabrescens Makino (SG), and Sigesbeckia orientalis L. (SO). S. orientalis has been used in Eastern medicine to alleviate rheumatic disorders, improve joint mobility, and provide protection against toxicity. This plant is naturally found in countries such as China, the Philippines, and Vietnam. In Türkiye, particularly in the Black Sea region, S. orientalis is known as Sarıteçan. In this study, the antioxidant capacity of the methanolic extract of S. orientalis was evaluated by determining the total phenolic content (TPMC) and total flavonoid content (TFC). The antioxidant activity was assessed using Ferric Reducing Antioxidant Power (FRAP), Cu (II) Ion Reducing Antioxidant Capacity (CUPRAC), and 2,2-Diphenyl-1-picrylhydrazyl Free Radical Scavenging Capacity (DPPH) assays. The total phenolic content (TPMC) of S. orientalis extract was determined to be 18 ± 0.57 mgGAE/g dry weight, and the total flavonoid content (TFC) was 8.55 ± 0.21 mg QE/g dry weight. The DPPH (SC50) was found to be 380 ± 12 µg/mL, while the CUPRAC and FRAP activities were 74 ± 1.99 and 19 ± 1.38 mg TE/g dry weight, respectively. These results demonstrate that S. orientalis grown in the Rize İkizdere region possesses strong antioxidant properties. Therefore, S. orientalis may have significant potential not only in traditional medicine but also in modern complementary medicine.

Keywords

Destekleyen Kurum

Herhangi bir kurum yada kuruluştan destek alınmamıştır.

Etik Beyan

Etik kurula ihtiyaç bulunmamaktadır.

Kaynakça

Alpkent, Z., & Demir, M. (2006). Gıdalarda Bulunan Antioksidan Maddeler ve İnsan Sağlığı Üzerine Etkileri. Türkiye 9. Gıda Kongresi. 24–26 Mayıs 2006, Bolu. Gıda Teknolojisi Derneği Yayın No 33.
Apak, R., Güçlü, K., Demirata, B., Özyürek, M., Çelik, S. E., Bektaşoǧlu, B., Berker, K. I., & Özyurt, D. (2007). Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12(7), 1496–1547.
Apak, R., Güçlü, K., Özyürek, M., & Çelik, S. E. (2008). Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta, 160, 413-419.
Asif, M. (2015). Chemistry and antioxidant activity of plants containing some phenolic compounds. Chemistry international, 1(1), 35-52.
Bayrak, Ç., Birinci, C., Kemal, M., Kolaylı, S. (2023). The Phenolic Composition and Antioxidant Properties of Figs (Ficus carica L.) Grown in the Black Sea Region. Plant Foods for Human Nutrition, 78(3), 539–545.
Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200. Cai, Y., Luo, Q., Sun, M., & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157-2184.
Chen, C. M., Houng, J. Y., Ko, T. L., Juan, S. H., Chou, H. C. (2022). Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways. Evidence-Based Complementary and Alternative Medicine, 2022(1), 3323745.

Choi, H. A., Ahn, S. O., Lim, H. D., & Kim, G. J. (2021). Growth suppression of a gingivitis and skin pathogen cutibacterium (Propionibacterium) acnes by medicinal plant extracts. Antibiotics, 10(9), 1092.
Christ, B., & Müller, K. H. (1960). Zur serienmäßigen Bestimmung des Gehaltes an Flavonol‐Derivaten in Drogen. Archiv der Pharmazie, 293(12), 1033-1042.
Çelik, Ş., Dervişoğlu, G., İzol, E., Sęczyk, Ł., Özdemir, F. A., Yilmaz, M. E., ... & Khan, M. A. (2024). Comprehensive phytochemical analysis of Salvia hispanica L. callus extracts using LC–MS/MS. Biomedical Chromatography, 38(10), e5975.
Dave, K. M., Darji, P. P., & Gandhi, F. R. (2019). Antimicrobial activity and phytochemical study of plant parts of Butea monosperma. Journal of Drug Delivery and Therapeutics, 9(4-A), 344-348.
Ejder, N. (2020). Rize'de yetişen bazı bitkilerden elde edilen özütlerin kanser ve normal hücreler üzerindeki etkilerinin in-vitro araştırılması (Tez no 656882).[Doktora Tezi, Recep Tayyip Erdoğan Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Folin, O. & Ciocalteau, V. (1927) Tyrosine and tryptophane in proteins. Journal of Biological Chemistry, 73(2), 627–648.
Gulcin, İ. (2020). Antioxidants and antioxidant methods: An updated overview. Archives of toxicology, 94(3), 651-715.
Güven, L., Ertürk, A., Yılmaz, M. A., Alwasel, S., & Gülçin, İ. (2024). Alchemilla pseudocartalinica Juz: Phytochemical Screening by UPLC-MS/MS, Molecular Docking, Anti-oxidant, Anti-diabetic, Anti-glaucoma, and Anti-Alzheimer Effects. Records of Natural Products, 18, 251-272.
Hung, W. C., Ling, X. H., Chang, C. C., Hsu, H. F., Wang, S. W., Lee, Y. C., ... & Houng, J. Y. (2017). Inhibitory effects of Siegesbeckia orientalis extracts on advanced glycation end product formation and key enzymes related to metabolic syndrome. Molecules, 22(10), 1785.
Junsathian, P., Nakamura, S., Katayama, S., & Rawdkuen, S. (2022). Antioxidant and antimicrobial activities of Thai edible plant extracts prepared using different extraction techniques. Molecules, 27(19), 6489.
Karagecili, H., Yılmaz, M. A., Ertürk, A., Kiziltas, H., Güven, L., Alwasel, S. H., & Gulcin, İ. (2023). Comprehensive metabolite profiling of Berdav propolis using LC-MS/MS: Determination of antioxidant, anticholinergic, antiglaucoma, and antidiabetic effects. Molecules, 28(4), 1739.
Karageçili, H., İzol, E., Kireçci, E., & Gülçin, İ. (2023). Antioxidant, antidiabetic, antiglaucoma, and anticholinergic effects of Tayfi grape (Vitis vinifera): A phytochemical screening by LC-MS/MS analysis. Open Chemistry, 21(1), 20230120.
Kendir, G. & Güvenç, A. (2010). Etnobotanik ve Türkiye ’ de Yapılmış Etnobotanik Çalışmalara Genel Bir Bakış. Hacettepe University Journal of the Faculty of Pharmacy, 30(1), 49–80.
Li, S., Li, S. K., Gan, R. Y., Song, F. L., Kuang, L., & Li, H. B. (2013). Antioxidant capacities and total phenolic contents of infusions from 223 medicinal plants. Industrial Crops and Products, 51, 289-298.
Linghu, K. G., Xiong, S. H., Zhao, G. D., Zhang, T., Xiong, W., Zhao, M., ... & Yu, H. (2020). Sigesbeckia orientalis L. extract alleviated the collagen type II–induced arthritis through inhibiting multi-target–mediated synovial hyperplasia and inflammation. Frontiers in pharmacology, 11, 547913.
Liu, M., Luo, T., Li, R., Yin, W., Yang, F., Ge, D., & Liu, N. (2025). Ent-pimarane and ent-kaurane diterpenoids from Siegesbeckia pubescens and their anti-endothelial damage effect in diabetic retinopathy. Chinese Journal of Natural Medicines, 23(2), 234-244.
Nguyen, T. D., Thuong, P. T., Hwang, I. H., Hoang, T. K., Nguyen, M. K., Nguyen, H. A., & Na, M. (2017). Anti-Hyperuricemic, Anti-Inflammatory and Analgesic Effects of Siegesbeckia orientalis L. Resulting from the Fraction with High Phenolic Content. BMC complementary and alternative medicine, 17(1), 191.
Öğretmen, Ö. Y. (2022) Scilla bifolia L. (orman sümbülü) bitki kısımlarının antioksidan aktivitesinin belirlenmesi. Journal of Anatolian Environmental and Animal Sciences, 7(1), 9–14.
Pradhan, S. K., Gupta, R. C., & Goel, R. K. (2018). Differential content of secondary metabolites in diploid and tetraploid cytotypes of Siegesbeckia orientalis L. Natural product research, 32(20), 2476-2482.
Ren, J., Yang, M., Chen, J., Ma, S., & Wang, N. (2020). Anti-inflammatory and wound healing potential of kirenol in diabetic rats through the suppression of inflammatory markers and matrix metalloproteinase expressions. Biomedicine & Pharmacotherapy, 129, 110475.
Sacan, O. (2018). Antioxidant activity, total phenol and total flavonoid contents of Trachystemon orientalis (L.) G. Don. European Journal of Biology, 77(2), 70-75.
Sukanya, M., Ashoka Babu, VL., & Azamthulla, M. (2022). "A detailed review on Siegesbeckia orientalis." Microscopy 8(9), 23-32.
Tao, H. X., Zhao, G. D., Linghu, K. G., Xiong, W., Sang, W., Peng, Y., ... & Yu, H. (2021). Botany, traditional use, phytochemistry, pharmacology and toxicology of Sigesbeckiae Herba (Xixiancao): a review. Phytochemistry Reviews, 20, 569-587.
Teixeira, T. S., Vale, R. C., Almeida, R. R., Ferreira, T. P. S., & Guimarães, L. G. L. (2017). Antioxidant potential and its correlation with the contents of phenolic compounds and flavonoids of methanolic extracts from different medicinal plants. Revista Virtual de Química, 9(4), 1546-1559.
Tohma, H., Köksal, E., Kılıç, Ö., Alan, Y., Yılmaz, M. A., Gülçin, İ., ... & Alwasel, S. H. (2016). RP-HPLC/MS/MS analysis of the phenolic compounds, antioxidant and antimicrobial activities of Salvia L. species. Antioxidants, 5(4), 38. https://doi.org/10.3390/antiox5040038.
Topal, M., & Gulcin, İ. (2022). Evaluation of the in vitro antioxidant, antidiabetic and anticholinergic properties of rosmarinic acid from rosemary (Rosmarinus officinalis L.). Biocatalysis and Agricultural Biotechnology, 43, 102417.
Tran, T. V. A., Pham, Q. T., Nguyen, V. K., Dao, V. H., Nguyen, T. H., Tran, V. T., ... & Nguyen, X. T. (2023). Simultaneous Quantification of Acid chlorogenic, Acid caffeic and Rutin in Herba Siegesbeckiae orientalis by HLPC Method. VNU Journal of Science: Medical and Pharmaceutical Sciences, 39(2), 2588-1132.
Yang, Y., Chen, H., Lei, J., & Yu, J. (2016). Biological activity of extracts and active compounds isolated from Siegesbeckia orientalis L. Industrial crops and products, 94, 288-293.
Yılmaz, M. A., Taslimi, P., Kılıç, Ö., Gülçin, İ., Dey, A., & Bursal, E. (2023). Unravelling the phenolic compound reserves, antioxidant and enzyme inhibitory activities of an endemic plant species, Achillea pseudoaleppica. Journal of Biomolecular Structure and Dynamics, 41(2), 445-456.
Zagoskina, N. V., Zubova, M. Y., Nechaeva, T. L., Kazantseva, V. V., Goncharuk, E. A., Katanskaya, V. M., ... & Aksenova, M. A. (2023). Polyphenols in plants: structure, biosynthesis, abiotic stress regulation, and practical applications. International Journal of Molecular Sciences, 24(18), 13874.
Zhou, Z. S., Wang, Z. J., Tian, B., Zhu, Y. Y., Wei, M. Z., Zhao, Y. L., & Luo, X. D. (2024). Three New Ent-Kaurane Diterpenes with Antibacterial Activity from Sigesbeckia orientalis. Molecules, 29(19), 4631. https://doi.org/10.3390/molecules29194631.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Farmasotik Botanik

Bölüm

Araştırma Makalesi

Yazarlar

Mehtap Atak ^*
0000-0002-9451-906X
Türkiye

Zeynep Bakan
0009-0001-7567-2544
Türkiye

Eda Yılmaz Kutlu
0000-0003-1211-4964
Türkiye

Merve Hüner Yiğit
0000-0001-8988-4321
Türkiye

Nebahat Ejder
0000-0001-9557-2033
Türkiye

Erken Görünüm Tarihi

10 Haziran 2025

Yayımlanma Tarihi

4 Temmuz 2025

Gönderilme Tarihi

3 Haziran 2024

Kabul Tarihi

17 Nisan 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1491528

IZ

https://izlik.org/JA96JE87BG

Kaynak Göster

RIS / Bibtex

APA

Atak, M., Bakan, Z., Yılmaz Kutlu, E., Hüner Yiğit, M., & Ejder, N. (2025). Antioxidant Properties of Methanolic Extract of Sigesbeckia orientalis L. from Rize. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(4), 1095-1102. https://doi.org/10.18016/ksutarimdoga.vi.1491528

Rize Yöresinde Yetişen Sigesbeckia orientalis L. Metanollü Ekstresinin Antioksidan Özellikleri

Öz

Anahtar Kelimeler

Antioxidant Properties of Methanolic Extract of Sigesbeckia orientalis L. from Rize

Abstract

Keywords

Destekleyen Kurum

Etik Beyan

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149