Mortality Effect of Origanum syriacum L. (Lamiaceae) and Satureja montana L. (Lamiaceae) Essential Oils on Pieris brassica L. (Lepidoptera: Pieridae)

Başak Ulaşlı; Nadire Pelin Bahadırlı; Zhanerke Amangeldı; Asime Filiz Çalışkan Keçe

doi:10.18016/ksutarimdoga.vi.1537664

EN TR

Mortality Effect of Origanum syriacum L. (Lamiaceae) and Satureja montana L. (Lamiaceae) Essential Oils on Pieris brassica L. (Lepidoptera: Pieridae)

Abstract

Cabbage, Brassica oleracea var. capitata L. (Brassicaceae), is one of the most widely consumed vegetables worldwide, and one of its most significant pests is Pieris brassicae L. (Lepidoptera: Pieridae). In recent years, the use of plant essential oils in pest control has significantly increased as an alternative control method. This study investigates the lethal effects of essential oils obtained from Origanum syriacum (Lamiaceae) and Satureja montana (Lamiaceae) on P. brassicae larvae. The main components of the essential oil of O. syriacum were identified as o-cymene (39.77%), carvacrol (28.16%), and thymol (12.78%), while the essential oil components of S. montana were determined as carvacrol (50.03%), o-cymene (15.12%), and γ-terpinene (13.02%). The essential oils were applied to different developmental stages (L1-L5) of P. brassicae using a spraying technique at various concentrations (5-10-20-50-100 µL mL⁻¹). The experiment was designed with 10 replicates, each containing 10 larvae from different developmental stages of P. brassicae, with water used as the control. To determine larval mortality rates, dead and living larvae were counted at 24-hour intervals for a total of 72 hours. The results showed that both S. montana and O. syriacum essential oils had a lethal effect ranging from 50% to 100%. However, S. montana essential oil was found to be more effective against P. brassicae larvae than O. syriacum essential oil.

Keywords

Origanum syriacum L. (Lamiaceae) ve Satureja montana L. (Lamiaceae) Uçucu Yağlarının Pieris brassicae L. (Lepidoptera: Pieridae) Üzerindeki Ölüm Etkisi

Öz

Lahana, Brassica oleracea var. capitata L. (Brassicaceae), tüm dünyada en fazla tüketimi yapılan sebzelerden birisi olup, en önemli zararlılarından birisi de Pieris brassicae L. (Lepidoptera: Pieridae)’dir. Son yıllarda zararlılarla mücadelede bitkisel uçucu yağların kullanılması alternatif mücadele yöntemi olarak önemli derecede artış göstermiştir. Bu çalışmada Origanum syriacum (Lamiaceae) ve Satureja montana (Lamiaceae) bitkilerinden elde edilen uçucu yağların P. brassicae larvaları üzerindeki ölüm etkisi araştırılmıştır. O. syriacum bitkisinin uçucu yağının ana bileşenleri o-cymene (%39.77), carvacrol (%28.16), ve thymol (%12.78) olarak belirlenmişken, S. montana’ nın uçucu yağ bileşenleri carvacrol (%50.03), o-cymene (%15.12) ve γ-Terpinene (%13.02) olarak belirlenmiştir. Uçucu yağlar, P. brassicae'nin (L1-L5) dönemlerine, farklı konsantrasyonlarda (5-10-20-50-100 µL mL-1) spreyleme tekniği kullanılarak uygulanmıştır. Deneme 10 tekerrür olacak şekilde kurulmuş, her tekerrürde P. brassicae'nin farklı larva dönemlerinden 10’ar adet larva ile kontrol olarak su kullanılmıştır. Larvaların ölüm oranını belirlemek için toplamda 72 saate kadar 24 saatlik aralıklarla ölü-canlı sayımı yapılmıştır. Hem S. montana hem de O. syriacum uçucu yağlarının %50-%100 oranlarında ölüm etkisine sahip olduğu, ancak S. montana uçucu yağının P. brassicae larvaları üzerinde O. syriacum uçucu yağından daha etkili olduğu tespit edilmiştir.

Anahtar Kelimeler

Kaynakça

Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide, J Econ Entomol, 18, 265-267.
Ahuja, B. B., Usharani, A., Srinivas, P., Singh, R. V., Sharma, P., & Bamawale, O. M. (2012). Development of farmerled integrated management of major pests of cauliflower cultivated in rainy season in India. Journal of Agricultural Sciences, 4(2), 79- 90.
Akdağcık, Z. (2010). Çukurova bölgesi crucıferae üretim alanlarında zararlı olan lepidopter türlerin populasyon gelişmeleri, predatör ve parazitoitlerinin belirlenmesi ve Pieris brassicae (L.)’nin bazı biyolojik özellikleri ile mücadelesi üzerine araştırmalar (Tez no 1285). [Doktora Tezi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü Bitki Koruma Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Al-Kalaldeh J. Z., Abu-Dahab R., & Afifi F. U. (2010). Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutrition Research, 30, 71–278.
Alma, M. H., Mavi, A., Yildirim, A., Digrak, M., & Hirata, T. (2003). Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biological and Pharmaceutical Bulletin, 26, 1725–1729.
Ali, S., Ullah, M. I., Arshad, M., Iftikhar, Y., Saqıb, M., & Afzal, M. (2017). Effect of botanicals and synthetic insecticides on Pieris brassicae (L., 1758) (Lepidoptera: Pieridae). Turkish Journal of Entomology, 41(3), 275-284.
Atar, H., & Çölgeçen, H. (2020). Bioactive compounds of Oregano seeds. Chapter 7 in: Nuts and Seeds in Health and Disease Prevention, Second Edition. https://doi.org/10.1016/B978-0-12-818553-7.00007-3.
Bahadirli, N. P. (2022). Comparison of Chemical Composition and Antimicrobial Activity of Salvia fruticosa Mill. and S. aramiensis Rech. Fill. (Lamiaceae). Journal of Essential Oil Bearing Plants, 25(4), 716–727. https://doi.org/10.1080/0972060X.2022.2110388

Baser, K. H. C., Kurkcuoglu, M., Demirci, B., & Ozek, T. (2003). The essential oil of Origanum syriacum L. var. sinaicum (Boiss.) letswaart. Flavour and Fragrance Journal,18, 98–99.
Bayhan, E., Ölmez, S., & Ulusoy, M. R. (2002). Doğu Akdeniz Bölgesi’nde lahana (Brassicae oleracae L.) ve karnabahar (Brassicae oleracae var. botrytis L.)’da zararlı olan türler ile bunların predatör ve parazitoitleri. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 17 (3), 85-92.
Bayhan, E., Bayhan, S. Ö., & Solhan, H. (2020). The Effect of Some Biological Parameters of Brevicoryne brassicae (L.) (Hemiptera: Aphididae) on Different Cauliflower Cultivars. International Journal of Scientific and Technological Research, 6(13), 69-74.
Bezic, N., Skocibusic, M., & Dunkic, V. (2005). Phytochemical composition and antimicrobial activity of Satureja montana L. and Satureja cuneifolia Ten. essential oils. Acta Botanica Croatica, 64 (2), 313–322.
Çeliktaş V, Otu Borlu H, Düzenli S., & Çalişkan Keçe A. F (2022). Aphis fabae (Hemiptera: Aphididae) mücadelesinde Laurus nobilis (Lauraceae) yağlarının etkinliğinin belirlenmesi. KSÜ Tarım ve Doğa Dergisi, 25 (6), 1361-1368. https://doi.org/10.18016/ksutarimdoga.vi.866824
Chorianopoulos N., Kalpoutzakis E., Aligiannis N., Mitaku S., Nychas G. J., & Haroutounian S. A. (2004). Essential oils of Satureja, Origanum, and Thymus species: Chemical composition and antibacterial activities against foodborne pathogens. Journal of Agricultural and Food Chemistry, 52, 8261–8267. doi: 10.1021/jf049113i.
Čopra-Janićijević, A., Vidic, D., & Maksimović, M. (2020). Characterisation of Satureja montana L. essential oil and headspace volatiles. Natural Volatiles and Essential Oils, 7(2), 22-34.
FAO, (2020). Uluslararası Gıda ve Tarım İstatistikleri (https://www.fao.org/faostat).
Dirmenci, T., Yazici, T., Özcan, T., Çelenk, S., & Martin, E. (2018). A new species and a new natural hybrid of Origanum L. (Lamiaceae) from the west of Turkey. Turkish Journal of Botany, 42(1), 73-90 (https://doi.org/10.3906/bot-1704-35).
Dirmenci, T., Yıldız, B., & Öztekin, M. (2019). A new record for the flora of Turkey: Satureja metastasiantha Rech. f. (Lamiaceae). Bağbahçe Bilim Dergisi, 6, 54-58.
Diome, T., Alioune, S., Faye, A., & Sembene, M. (2019). Biocidal activity of Crataeva religiosa based substances against the major Lepidoptera cabbage pests. Journal of Entomology and Zoology Studies, 7 (3), 1524-1528.
Duman, H.; Başer, K. H. C., & Aytaç, Z. (1998). Two New Species and A New Hybrid from Anatolia. Turkish Journal of Botany., 22 (1), 51-55 (https://journals.tubitak.gov.tr/botany/vol22/iss1/8).
Duman, H., Dı̇rmencı̇, T., & Özcan, T. (2023). A new annual Satureja (Lamiaceae) species from Turkey with molecular evidence, and lectotypification of two species. Turkish Journal of Botany., 47(1), 61-72 https://doi.org/10.55730/1300-008X.2744.
El-Alam, I., Zgheib, R., Iriti, M, El Beyrouthy, M., Hattouny, P., Verdin, A.; Fontaine, J., Chahine, R., Lounes-Hadj Sahraoui, A., & Makhlouf, H. (2019). Origanum syriacum Essential Oil Chemical Polymorphism According to Soil Type. Foods, 8, 90.
Eliuz, E., & Bahadirli, N. P. (2022). Anticandidal Activity and Anticandidal Mechanism of Essential Oil of Cuminum cyminum L. and Myrtus communis L. Mixture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25 (Ek Sayı 2), 391-401.
Eroğlu, B. G., Bakirdogen, A. M., Yazıcılar, B., & Atıcı, Ö. (2023). Investigation of Changes in Expression Levels of Detoxification Genes and Mortality in Pieris brassicae Larvae Exposed to Nepeta meyeri. Biology Bulletin of the Russian Academy of Sciences, 50, 784–789. https://doi.org/10.1134/S1062359023601477.
Firake, D. M., Thubru, D. P., & Behere, G. T. (2017). Eco-toxicological risk and impact of pesticides on important parasitoids of cabbage butterflies in cruciferous ecosystem, Chemosphere, 168, 372-383.
Fleisher, A., & Fleisher, Z. (1991). Chemical Composition of Origanum syriacum L. Essential Oil. Journal of Essential Oil Research, 3, 121–123.
Gendy, A. N. E., Leonardi, M., Mugnaini, L., Bertelloni, F., Ebani, V. V., Nardoni, S., Mancianti, F., Hendawy, S., Omer, E., & Pistelli, L. (2015). Chemical composition and antimicrobial activity of essential oil of wild and cultivated Origanum syriacum plants grown in Sinai, Egypt. Industrial Crops and Products, 67, 201–207.
Hasan, F., & Ansari, M. S. (2010). Effect of different crops on the biological parameters of Pieris brassicae (L.) (Lepidoptera: Pieridae) under laboratory conditions. Journal of Crop Science and Biotechnology, 13 (3),195-202
Hossain, Md. E., Rahman, Md. M., Akter, S., Akhter, N., & Khan, Md. S. I. (2020). Botanicals Affect the Incidence of Lepidopteran Insect Pests on Summer Cabbage. International Journal of Business, Social and Scientific Research, 8(3), 15–19. Retrieve from http://www.ijbssr.com/currentissueview/14013363
Isman, M. B. (1997). Neem and other botanical insecticides banlers to commercialization. Phytoparasitica, 25, 339–344.
Kaleeswaran, G., Firake, D. M., Behere, G. T., Challa, G. K., Sanjukta, R. K., & Baiswar, P. (2019). Insecticidal potential of traditionally important plant, Zanthoxylum armatum DC (Rutaceae) against cabbage butterfly, Pieris brassicae (Linnaeus). Indian Journal of Traditional Knowledge,18 (2), 304-311.
Kardian, A., & Maris, P. (2021). Effect of botanical insecticides against Fall armyworm Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae). IOP Conf. Ser.: Earth Environ. Sci., 653, 012060.
Kaya, K., & Kornoşor, S. (2008). The lepidopterous pest species, their parasitoids and population dynamics of the important ones in winter vegetables areas in Hatay province. The Turkish Journal of Entomology, 32 (3), 195-209.
Meyers, M. (2005). Oregano and Marjoram An Herb Society of America Guide to the Genus Origanum; The Herb Society of America: Kirtland, OH, USA.
Mondédji, A. D., Silvie, P., Nyamador, W. S., Martin, P., Agboyi, L. K., Amévoin, K., Ketoh, G. K., & Glitho, I. A. (2021). Cabbage Production in West Africa and IPM with a Focus on Plant-Based Extracts and a Complementary Worldwide Vision. Plants, 10, 529. https:// doi.org/10.3390/plants10030529.
Mordue, A. J., Simmonds, M. S. J., Ley, S.V., Blaney, W. M., Mordue, W., Nasiruddin, M., & Nisbet, A. J. (1998). Actions of azadirachtin, a plant allelochemical, against insects. Pesticide Science, 54, 277-284.
Paudel, A., Yadav, P. K., & Karna, P. (2022). Diamondback Moth Plutella xylostella (Linnaeus,1758) (Lepidoptera: Plutellidae); A Real Menace to Crucifers and Its Integrated Management Tactics. Turkish Journal of Agriculture-Food Science and Technology, 10 (12), 2504-2515.
Redzic, S., Tuka, M., & Pajević, A. (2006). Research into microscopic structure and essential oils of endemic medicinal plant species Satureja subspicata Bartl. Ex Vis. (Lamiaceae). The Bosnian Journal of Basic Medical Sciences, 6, 25–31.
Shehadeh, M., Jaradat, N., Al-Masri, M., Zaid, A. N., Hussein, F., Khasati, A., Suaifan, G., & Darwish, R. (2019). Rapid, cost-effective and organic solvent-free production of biologically active essential oil from Mediterranean wild Origanum syriacum. The Saudi Pharmaceutical Journal, 27, 612–618.
TÜİK, (2022). Türkiye İstatistik Kurumu. https://www.tuik.gov.tr/. Son erişim tarihi: 21.08.2023
Ujvary, I. (2001). Pest Control Agents from Natural Products. In handbook of pesticide toxicology, 23rd (Editör: R. I. Krieger) Academic Press. San Diego, 109-179.
Ulusoy, M. R. & Bayhan, S. Ö. (2006). Effect of certain Brassica plants on biology of the cabbage aphid Brevicoryne brassicae under laboratory conditions. Phytoparasitica, 34(2), 133-138.
Viuda-Martos, M., El Gendy Ael, N., Sendra, E., Fernández-López, J., Abd El Razik, K. A., Omer, E. A., & Pérez- Alvarez, J.A. (2010). Chemical composition and antioxidant and anti-Listeria activities of essential oils obtained from some Egyptian plants. Journal of Agricultural and Food Chemistry, 58, 9063–9070.
Walia, S., Saha, S., Tripathi, V., & Sharma, K. K. (2017). Phytochemical biopesticides: some recent developments. Phytochemistry Reviews, 16, 989–1007. DOI 10.1007/s11101-017-9512-6.
Zakaria A-N. (2016). Analysis of essential oil of Capparis spinosa L. leaves andinteraction between Pieris brassicae L. (Lepidopteran) which attack caper and natural enemy Cotesia glomerata (L.). International Journal of ChemTech Research, 9(7), 477-485. CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Entomoloji

Bölüm

Araştırma Makalesi

Yazarlar

Başak Ulaşlı ^*
0000-0002-0989-2020
Türkiye

Nadire Pelin Bahadırlı
0000-0001-7321-5377
Türkiye

Zhanerke Amangeldı
0000-0002-9017-8106
Türkiye

Asime Filiz Çalışkan Keçe
0000-0002-9330-1958
Türkiye

Erken Görünüm Tarihi

1 Mayıs 2025

Yayımlanma Tarihi

9 Haziran 2025

Gönderilme Tarihi

23 Ağustos 2024

Kabul Tarihi

22 Mart 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1537664

IZ

https://izlik.org/JA44UH55RC

Kaynak Göster

RIS / Bibtex

APA

Ulaşlı, B., Bahadırlı, N. P., Amangeldı, Z., & Çalışkan Keçe, A. F. (2025). Mortality Effect of Origanum syriacum L. (Lamiaceae) and Satureja montana L. (Lamiaceae) Essential Oils on Pieris brassica L. (Lepidoptera: Pieridae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 797-806. https://doi.org/10.18016/ksutarimdoga.vi.1537664